Abstract
The MaMV-DC cyanophage, which infects the bloom-forming cyanobacterium Microcystis aeruginosa, was isolated from Lake Dianchi, Kunming, China. Twenty-one cyanobacterial strains were used to detect the host range of MaMV-DC. Microcystic aeruginosa FACHB-524 and plaque purification were used to isolate individual cyanophages, and culturing MaMV-DC with cyanobacteria allowed us to prepare purified cyanophages for further analysis. Electron microscopy demonstrated that the negatively stained viral particles are tadpole-shaped with an icosahedral head approximately 70 nm in diameter and a contractile tail approximately 160 nm in length. Using one-step growth experiments, the latent period and burst size of MaMV-DC were estimated to be 24–48 hours and approximately 80 infectious units per cell, respectively. Restriction endonuclease digestion and agarose gel electrophoresis were performed using purified MaMV-DC genomic DNA, and the genome size was estimated to be approximately 160 kb. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis revealed four major structural proteins. These results support the growing interest in using freshwater cyanophages to control bloom-forming cyanobacterium.
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References
Ackermann H W. 2007. 5500 phages examined in the electron microscope. Arch Virol, 152: 227–243.
Brussaard C P. 2004. Viral control of phytoplankton populations — a review. J Eukaryot Microbiol, 51: 125–138.
Chen J, Zhang D, Xie P, Wang Q, and Ma Z. 2009. Simultaneous determination of microcystin contaminations in various vertebrates (fish, turtle, duck and water bird) from a large eutrophic Chinese lake, Lake Taihu, with toxic Microcystis blooms. Science of the Total Environment, 407: 3317–3322.
Davis T W, Berry D L, Boyer G L, and Gobler C J. 2009. The effects of temperature and nutrients on the growth and dynamics of toxic and non-toxic strains of Microcystis during cyanobacteria blooms. Harmful algae, 8: 715–725.
Gao E B, Gui J F, and Zhang Q Y. 2012. A novel cyanophage with a cyanobacterial nonbleaching protein a gene in the genome. J Virol, 86: 236–245.
Gao E B, Yuan X P, Li R, and Zhang Q Y. 2009. Isolation of a novel cyanophage infectious to the filamentous cyanobacterium planktothrix agardhii(cyanophyceae) from lake donghu, china. Aquat Microb Ecol, 54: 163–170.
Liu Y M, Yuan X P, and Zhang Q Y. 2006. Spatial distribution and morphologic diversity of virioplankton in lake donghu, china. Acta Oecol, 29: 328–334.
Oliver R L, and Ganf G G. 2002. Freshwater blooms. In: A. Whitton, M. Potts Eds. The ecology of cyanobacteria, Springer Netherlands, pp 149–194.
Safferman R S, Cannon R E, Desjardins P R, Gromov B V, and others.1983. Classification and nomenclature of viruses of cyanobacteria. Intervirology, 19: 61–66.
Safferman R S, and Morris M E. 1963. Algal virus isolation. Science, 140: 679–680.
Sengco M R. 2009. Prevention and control of Karenia brevis blooms. Harmful Algae, 8: 623–628.
Suttle C A, and Chan A M. 1993. Marine cyanophages infecting oceanic and coastal strains of Synechococcus: abundance, morphology, cross-infectivity and growth characteristics. Mar Ecol-Prog Ser, 92: 99–109.
Tan X, Kong F X, Yu Y, and Zhang M. (2009). Spatio-temporal variations of phytoplankton community composition assayed by morphological observation and photosynthetic pigment analyses in Lake Taihu (China). Afr J Biotechnol, 8: 4977–4982.
Wichels A, Gerdts G, and Schütt C. 2002. Pseudoalteromonas phages, a significant group of marine bacteriophages in the north sea. Aquat Microb Ecol, 27: 233–239.
Wood S A, Kuhajek J M, Winton M, and Phillips N R. 2012. Species composition and cyanotoxin production in periphyton mats from three lakes of varying trophic status. FEMS Microbiol Ecol, 79: 312–326.
Wu Z X, Gan N Q, and Song L R. 2007. Genetic diversity: Geographical distribution and toxin profiles of Microcystis strains (Cyanobacteria) in China. J Integr Plant Biol, 49: 262–269.
Yang F, Wei H Y, Li X Q, Li Y H, Li X B, Yin L H, and Pu Y P. 2013. Isolation and Characterization of an Algicidal Bacterium Indigenous to Lake Taihu with a Red Pigment able to Lyse Microcystis aeruginosa. Biomed Environ Sci, 26: 148–154.
Yoshida M, Yoshida T, Kashima A, Takashima Y, Hosoda N, Nagasaki N, and Hiroishi S. 2008. Ecological dynamics of the toxic bloom-forming cyanobacterium Microcystis aeruginosa and its cyanophages in freshwater. Appl Environ Microbiol, 74: 3269–3273.
Yoshida T, Takashima Y, Tomaru Y, Shirai Y, and others. 2006. Isolation and characterization of a cyanophage infecting the toxic cyanobacterium microcystis aeruginosa. Appl Environ Microb, 72: 1239–1247.
Zhang Q Y, and Gui J F. 2008. Viriplankton. In: Zhang Q Y, Gui J F. Eds. Aquatic Virology, Beijing, Higher Education Press, pp 297–350.
Zhang Q Y, and Gui J F. 2009. One kind of strategic bio-resources that cannot be ignored — Freshwater and marine viruses and their roles in the global ecosystem. Bulletin of Chinese Academy of Sciences, 24:520–526 (Chinese with English abstract).
Zhang Q Y, and Gui J F. 2012. Atlas of Aquatic Viruses and Viral Diseases. Beijing, Science Press. pp 404–460.
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Ou, T., Li, S., Liao, X. et al. Cultivation and characterization of the MaMV-DC cyanophage that infects bloom-forming cyanobacterium Microcystis aeruginosa . Virol. Sin. 28, 266–271 (2013). https://doi.org/10.1007/s12250-013-3340-7
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DOI: https://doi.org/10.1007/s12250-013-3340-7