Development and characterization of West Nile virus replicon expressing secreted Gaussia Luciferase
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We developed a Gaussia luciferase (Gluc) reporter replicon of West Nile virus (WNV) and used it to quantify viral translation and RNA replication. The advantage of the Gluc replicon is that Gaussia luciferase is secreted into the culture medium from cells transfected with Gluc replicon RNA, and the medium can be assayed directly for luciferase activity. Using a known Flavivirus inhibitor (NITD008), we demonstrated that the Gluc-WNV replicon could be used for antiviral screening. The Gluc-WNV-Rep will be useful for research in antiviral drug development programs, as well as for studying viral replication and pathogenesis of WNV.
KeywordsFlavivirus Replicon West Nile virus High-throughput assay
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- Gubler D, Kuno, G., Markoff, L. (ed.). 2007. Fields Virology.Google Scholar
- Lo M K, Tilgner M, Bernard K A, and Shi P Y. 2003. Functional Analysis of Mosquito-Borne Flavivirus Conserved Sequence Elements within 3′ Untranslated Region of West Nile Virus by Use of a Reporting Replicon That Differentiates between Viral Translation and RNA Replication. Journal of Virology, 77: 10004–10014.PubMedCrossRefGoogle Scholar
- Yin Z, Chen Y L, Schul W, Wang Q Y, Gu F, Duraiswamy J, Kondreddi R R, Niyomrattanakit P, Lakshminarayana S B, Goh A, Xu H Y, Liu W, Liu B, Lim J Y H, Ng C Y, Qing M, Lim C C, Yip A, Wang G, Chan W L, Tan H P, Lin K, Zhang B, Zou G, Bernard K A, Garrett C, Beltz K, Dong M, Weaver M, He H, Pichota A, Dartois V, Keller T H, and Shi P Y. 2009. An adenosine nucleoside inhibitor of dengue virus. Proceedings of the National Academy of Sciences, 106: 20435–20439.CrossRefGoogle Scholar