Advertisement

Virologica Sinica

, Volume 28, Issue 1, pp 36–42 | Cite as

An inactivated vaccine from a field strain of bovine herpesvirus-1 (BoHV-1) has high antigenic mass and induces strong efficacy in a rabbit model

  • Julian Ruiz-SáenzEmail author
  • Jairo Jaime
  • Victor Vera
Research Article

Abstract

Bovine Herpesvirus-1 (BoHV-1) is a DNA virus belonging to the family Herpesviridae, subfamily Alfaherpesvirinae; it is a worldwide pathogen, causing serious economic losses in livestock. In Colombia there have been multiple isolates of BoHV-1 that have been subjected to molecular characterization, classifying most of the country isolates as BoHV-1.1. In the present study we developed and evaluated an ethyleneimine binary inactivated isolate from the native BoHV-1 strain (Córdoba-2) in a rabbit model of vaccination and infection. The vaccine was evaluated in two phases, one of immunogenicity with vaccination and a booster after 21 days, and an evaluation phase of protection against challenge with a highly virulent reference strain. The results demonstrate optimum serum-conversion, with protective neutralizing antibody titers 28 days post vaccination and optimal protection against challenge with the reference strain with decreased clinical signs of infection, protection against the onset of fever and decrease of virus excretion post challenge. In conclusion, our results show the enormous potential that an immunogenic inactivated vaccine has produced from the native BoHV-1.1 strain, which produces a high antigen mass to the vaccine to induce optimal immunity and protection, and it is a strong candidate for evaluation and possible future use in different cattle populations.

Keywords

Antigenic Mass Adjuvant Binary Ethyleneimine Bovine Herpesvirus-1 Vaccine 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bahnemann H G. 1990. Inactivation of viral antigens for vaccine preparation with particular reference to the application of binary ethylenimine. Vaccine, 8: 299–303.PubMedCrossRefGoogle Scholar
  2. Caron L, Flores E F, Weiblen R, Scherer C F, Irigoyen L F, Roehe P M, Odeon A and Sur J H. 2002. Latent infection by bovine herpesvirus type-5 in experimentally infected rabbits: virus reactivation, shedding and recrudescence of neurological disease. Vet Microbiol, 84: 285–295.PubMedCrossRefGoogle Scholar
  3. Chung H, Kim T W, Kwon M, Kwon I C and Jeong S Y. 2001. Oil components modulate physical characteristics and function of the natural oil emulsions as drug or gene delivery system. J Control Release, 71: 339–350.PubMedCrossRefGoogle Scholar
  4. Davison A J, Eberle R, Ehlers B, Hayward G S, McGeoch D J, Minson A C, Pellett P E, Roizman B, Studdert M J and Thiry E. 2009. The order Herpesvirales. Arch Virol, 154: 171–177.PubMedCrossRefGoogle Scholar
  5. DesCoteaux L, Cecyre D, Elsener J and Beauchamp G. 2003. Comparison of humoral immune responses in dairy heifers vaccinated with 3 different commercial vaccines against bovine viral diarrhea virus and bovine herpesvirus-1. Can Vet J, 44: 816–821.PubMedGoogle Scholar
  6. Duque H, Marshall R L, Israel B A and Letchworth G J. 1989. Effects of formalin inactivation on bovine herpes virus-1 glycoproteins and antibody response elicited by formalin-inactivated vaccines in rabbits. Vaccine, 7: 513–520.PubMedCrossRefGoogle Scholar
  7. Engels M, Steck F and Wyler R. 1981. Comparison of the genomes of infectious bovine rhinotracheitis and infectious pustular vulvovaginitis virus strains by restriction endonuclease analysis. Arch Virol, 67: 169–174.PubMedCrossRefGoogle Scholar
  8. Hierholzer J C and Killington R A. 1996. Virus isolation and quantitation, p. 25–46. In Mahy B W J and Kangro H O (ed.), Virology methods manual. Academic Press, London.CrossRefGoogle Scholar
  9. Ioannou X P, Griebel P, Hecker R, Babiuk L A and van Drunen Littel-van den Hurk S. 2002. The immunogenicity and protective efficacy of bovine herpesvirus 1 glycoprotein D plus Emulsigen are increased by formulation with CpG oligodeoxynucleotides. J Virol, 76: 9002–9010.PubMedCrossRefGoogle Scholar
  10. Kamaraj G, Rana S K and Srinivasan V A. 2009. Serological response in cattle immunized with inactivated oil and Algel adjuvant vaccines against infectious bovine rhinotracheitis. New Microbiol, 32: 135–141.PubMedGoogle Scholar
  11. Lesko J, Veber P, Hrda M and Feketeova M. 1993. Large-scale production of infectious bovine rhinotracheitis virus in cell culture on microcarriers. Acta Virol, 37: 73–78.PubMedGoogle Scholar
  12. Miller J M, Whetstone C A, Bello L J and Lawrence W C. 1991. Determination of ability of a thymidine kinase-negative deletion mutant of bovine herpesvirus-1 to cause abortion in cattle. Am J Vet Res, 52: 1038–1043.PubMedGoogle Scholar
  13. Muylkens B, Meurens F, Schynts F, Farnir F, Pourchet A, Bardiau M, Gogev S, Thiry J, Cuisenaire A, Vanderplasschen A and Thiry E. 2006. Intraspecific bovine herpesvirus 1 recombinants carrying glycoprotein E deletion as a vaccine marker are virulent in cattle. J Gen Virol, 87: 2149–2154.PubMedCrossRefGoogle Scholar
  14. OIE. 2004. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, 5th ed. OIE, Paris, France.Google Scholar
  15. Parreno V, Lopez M V, Rodriguez D, Vena M M, Izuel M, Filippi J, Romera A, Faverin C, Bellinzoni R, Fernandez F and Marangunich L. 2010. Development and statistical validation of a guinea pig model for vaccine potency testing against Infectious Bovine Rhinothracheitis (IBR) virus. Vaccine, 28: 2539–2549.PubMedCrossRefGoogle Scholar
  16. Pastoret P P and Thiry E. 1985. Diagnosis and prophylaxis of infectious bovine rhinotracheitis: the role of virus latency. Comp Immunol Microbiol Infect Dis, 8: 35–42.PubMedCrossRefGoogle Scholar
  17. Patel J R. 2004. Evaluation of a quadrivalent inactivated vaccine for the protection of cattle against diseases due to common viral infections. J S Afr Vet Assoc, 75: 137–146.PubMedGoogle Scholar
  18. Patel J R. 2005. Relative efficacy of inactivated bovine herpesvirus-1 (BHV-1) vaccines. Vaccine, 23: 4054–4061.PubMedCrossRefGoogle Scholar
  19. Peters A R, Thevasagayam S J, Wiseman A and Salt J S. 2004. Duration of immunity of a quadrivalent vaccine against respiratory diseases caused by BHV-1, PI3V, BVDV and BRSV in experimentally infected calves. Prev Vet Med, 66: 63–77.PubMedCrossRefGoogle Scholar
  20. Rossi C R and Kiesel G K. 1982. Effect of infectious bovine rhinotracheitis virus immunization on viral shedding in challenge-exposed calves treated with dexamethasone. Am J Vet Res, 43: 1576–1579.PubMedGoogle Scholar
  21. Ruiz-Saenz J, Jaime J and Vera V. 2009. Bovine Herpesvirus-1 Vaccine’s: A Look From The Past To The Immunization Future. Acta biol Colomb, 14: 3–20.Google Scholar
  22. Ruiz-Saenz J, Jaime J, Ramirez G and Vera V. 2012. Molecular and in vitro characterization of field isolates of bovine herpesvirus-1. Virol Sin, 27: 26–37.PubMedCrossRefGoogle Scholar
  23. Schudel A A, Carrillo B J, Wyler R and Metzler A E. 1986. Infections of calves with antigenic variants of bovine herpesvirus 1 (BHV-1) and neurological disease. Zentralbl Veterinarmed B, 33: 303–310.PubMedGoogle Scholar
  24. Toussaint J F, Letellier C, Paquet D, Dispas M and Kerkhofs P. 2005. Prime-boost strategies combining DNA and inactivated vaccines confer high immunity and protection in cattle against bovine herpesvirus-1. Vaccine, 23: 5073–5081.PubMedCrossRefGoogle Scholar
  25. Valera A R, Pidone C L, Massone A R, Quiroga M A, Riganti J G, Corva S G and Galosi C M. 2008. A simple method of infecting rabbits with Bovine herpesvirus 1 and 5. J Virol Methods, 150: 77–79.PubMedCrossRefGoogle Scholar
  26. Zimmerman A D, Buterbaugh R E, Herbert J M, Hass J M, Frank N E, Luempert Iii L G and Chase C C. 2007. Efficacy of bovine herpesvirus-1 inactivated vaccine against abortion and stillbirth in pregnant heifers. J Am Vet Med Assoc, 231: 1386–1389.PubMedCrossRefGoogle Scholar

Copyright information

© Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Microbiology and Epidemiology Group, Faculty of Veterinary Medicine and Animal ScienceNational University of ColombiaBogotáColombia
  2. 2.Grupo de Investigación CENTAURO, Agrarian Sciences FacultyUniversidad de AntioquiaMedellínColombia

Personalised recommendations