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Virologica Sinica

, Volume 27, Issue 5, pp 273–277 | Cite as

Rapid detection of filoviruses by real-time TaqMan polymerase chain reaction assays

  • Yi Huang
  • Hongping Wei
  • Yunpeng Wang
  • Zhengli Shi
  • Herve Raoul
  • Zhiming Yuan
Research Article

Abstract

Ebola virus (EBOV) and Marburg virus (MARV) are causative agents of severe hemorrhagic fever with high mortality rates in humans and non-human primates and there is currently no licensed vaccine or therapeutics. To date, there is no specific laboratory diagnostic test in China, while there is a national need to provide differential diagnosis during outbreaks and for instituting acceptable quarantine procedures. In this study, the TaqMan RT-PCR assays targeting the nucleoprotein genes of the Zaire Ebolavirus (ZEBOV) and MARV were developed and their sensitivities and specificities were investigated. Our results indicated that the assays were able to make reliable diagnosis over a wide range of virus copies from 103 to 109, corresponding to the threshold of a standard RNA transcript. The results showed that there were about 1010 RNA copies per milliliter of virus culture supernatant, equivalent to 10,000 RNA molecules per infectious virion, suggesting the presence of many non-infectious particles. These data indicated that the TaqMan RT-PCR assays developed in this study will be suitable for future surveillance and specific diagnosis of ZEBOV and MARV in China.

Keywords

Ebola virus Marburg virus Nucleoprotein (NP) gene TaqMan RT-PCR 

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Copyright information

© Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Yi Huang
    • 1
  • Hongping Wei
    • 1
  • Yunpeng Wang
    • 1
  • Zhengli Shi
    • 1
  • Herve Raoul
    • 2
  • Zhiming Yuan
    • 1
  1. 1.State Key Laboratory of Virology, Wuhan Institute of VirologyChinese Academy of SciencesWuhanChina
  2. 2.Laboratoire P4 Jean MérieuxNational Institute for Health and Medical Research (Inserm)Lyon Cedex 07France

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