Abstract
Enterovirus 71 (EV71) is a member of the Entero-virus genus of the Picornaviridae family and is the major cause of Hand, foot, and mouth disease (HFMD) in children. Different strains from Gansu were cloned and the P1 protein was sequenced and analysed. Results indicate that there are three kinds of EV71 infections prevalent in Gansu. The VP1 protein from one of these strains, 55F, was expressed. The recombinant protein was expressed with high level and reacted specifically with the EV71 patient antibody, the recombinant protein was also applied to raise antiserum in rabbits and after the fourth injection a high titer of antiserum was detected by ELISA assay. These data are useful for further clarification of prevalent EV71 strains in the north of China at the molecular level and provide a basis for EV71 diagnosis.
References
Bible J M, Iturriza-Gomara M, Megson B, et al. 2006. Molecular epidemiology of human enterovirus71 in the UnitedKingdomfrom1998 to 2006. J Clin Microbiol. 46:3192–3200.
Brown B A, Oberste M S, Alexander Jr J P, et al. 1999. Molecular epidemiology and evolution of enterovirus71 strains isolated from1970 to 1998. J Virol. 73(12):9969–9975.
Chan Y F, Sam I-Ch, AbuBaka S. 2010. Phylogenetic designation of enterovirus 71 genotype sand subgenotype susing complete genome sequences. Infect Genet Evol, 10: 404–412.
Chen H L, Huang J Y, Chu T W, et al. 2008. Expression of VP1 protein in the milk of transgenic mice: A potential oral vaccine protects against enterovirus 71 infection. Vaccine, 26(23): 2882–2889.
Chumakov M, Voroshilova M, Shindarov L, et al. 1979. Enterovirus 71 isolated from cases of epidemic poliomyelitis-like disease in Bulgaria. Arch Virol, 60: 329–340.
Diedrich S, Weinbrecht A, Schreier E. 2009. Seroprevalence and molecula repidemiology of enterovirus71 in Germany. Arch Virol, 154:1139–1142.
Foo D G, Alonso S, Phoon M C, et al. 2007. Identification of neutralizing linear epitopes from the VP1 capsid protein of Enterovirus 71 using synthetic peptides.Virus Res, 125:61–68.
He Y H, Yang F, Li C L, et al. 1999. Identification of EV71 isolates from hand, foot and mouth disease cases in Shenzhen. Chin J Exp Clin Virol, 1: 112. (in Chinese)
Li X L, Mao C M, Ma S J, et al. 2009. Generation of neutralizing monoclonal antibodies against Enterovirus71 using syntheticpeptides. Biochem Biophys Res Commun, 390:1126–1128.
Moore D M, Cowan K M. 1978. Effect of trypsin and chymotrypsin on the polypeptides of large and small plaque variants of foot-and-mouth disease virus: relationship to specific antigenicity and infectivity. J Gen Virol, 41:549–562.
Ortner B, Huang C W, Schmid D, et al. 2009. Epidemiology of entero-virus types causing neurological disease in Austria 1999–2007: detection of clusters of echovirus30 and enterovirus71 and anal-ysis of prevalent genotypes. J Med Virol, 81:317–324.
Peter C M. 2002. An overview of the evolution of enterovirus 71 and its clinical and public health significance.FEMS Microbiol Rev. 26: 91–107.
Peter M, Katie L, David P, et al. 2001. Phylogenetic Analysis of Enterovirus 71 Strains Isolated during Linked Epidemics in Malaysia, Singapore, and Western Australia. J Virol, 75: 7732–7738.
Sanders S A, Herrero L J, McPhie K, et al. 2006. Molecular epidemiology of enterovirus71 over two decades in an Australian urban community. Arch Virol, 151: 1003–1013.
Shih S R, Li Y S, shuen P C, et al. 2000. Expression of capsid protein VP1 for use as antigen for the diagnosis of enterovirus 71 infection. J Med Virol, 61:228–34.
Tung W S, Bakar S A, Sekawi Z, et al. 2007. DNA vaccine constructs against enterovirus 71 elicit immune response in mice. Genet Vaccines Ther, 5:6.
Yu H Y, Chen W, Chang H Y, et al. 2010. Genetic anylysis of the VP1 region of ecterovirus 71 reveals the emergence of genotype A in central China in 2008. Virus genes. 41:1–4.
Zhou S L, Li L L, He Y Q, et al. 2007. Expression of VP1 of Enterovirus 71 in E. coli. J Anal Sci. 23:157–159. (in Chinese)
Author information
Authors and Affiliations
Corresponding author
Additional information
Foundation item: Major S&T Special Projects of Gansu (092GKDA010)
Rights and permissions
About this article
Cite this article
Li, Jq., Yang, Jj., Fan, Xj. et al. Genetic analysis of the P1 region of human enterovirus 71 strains and expression of the 55 F strainVP1 protein. Virol. Sin. 27, 10–18 (2012). https://doi.org/10.1007/s12250-012-3206-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12250-012-3206-4
Key words
- EV71
- Genetic analysis
- P1 region
- Expression
- VP1 protein