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Ribosome inactivating proteins from plants inhibiting viruses

Virologica Sinica volume 26, pages 357–365 (2011)Cite this article

Abstract

Many plants contain ribosome inactivating proteins (RIPs) with N-glycosidase activity, which depurinate large ribosomal RNA and arrest protein synthesis. RIPs so far tested inhibit replication of mRNA as well as DNA viruses and these proteins, isolated from plants, are found to be effective against a broad range of viruses such as human immunodeficiency virus (HIV), hepatitis B virus (HBV) and herpes simplex virus (HSV). Most of the research work related to RIPs has been focused on antiviral activity against HIV; however, the exact mechanism of antiviral activity is still not clear. The mechanism of antiviral activity was thought to follow inactivation of the host cell ribosome, leading to inhibition of viral protein translation and host cell death. Enzymatic activity of RIPs is not limited to depurination of the large rRNA, in addition they can depurinate viral DNA as well as RNA. Recently, Phase I/II clinical trials have demonstrated the potential use of RIPs for treating patients with HIV disease. The aim of this review is to focus on various RIPs from plants associated with anti-HIV activity.

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References

  1. Barbieri L, Ferreras J M, Barraco A, et al. 1992. Some ribosome-inactivating proteins depurinate ribosomal RNA at multiple sites. Biochem J, 286: 1–4.

    PubMed  CAS  Google Scholar 

  2. Bica I, McGovern B, Dhar R, et al. 2001. Increasing mortality due to end-stage liver disease in patients with human immunodeficiency virus infection. Clin Infect Dise, 132: 492–497.

    Article  Google Scholar 

  3. Bourinbaiar A S, Huang S L. 1996. The activity of plant-derived antiretroviral proteins MAP30 and GAP31 against Herpes Simplex Virus infection in vitro. Biochem Biophys Res Commun, 219: 923–929.

    Article  PubMed  CAS  Google Scholar 

  4. Byers V S, Levin A S, Waites L A, et al. 1990. A phase I/II study of trichosanthin treatment of HIV disease. AIDS, 4: 1189–1196.

    Article  PubMed  CAS  Google Scholar 

  5. D’cruz O J, Uckun F M. 2001. Pokeweed antiviral protein: a potential nonspermicidal prophylactic antiviral agent. Fertil Steril, 75: 106–114.

    Article  PubMed  Google Scholar 

  6. Fan J M, Zhang Q, Xu J, et al. 2009. Inhibition on hepatitis B virus in vitro of recombinant MAP30 from bitter melon. Mol Biol Rep, 36: 381–388.

    Article  PubMed  CAS  Google Scholar 

  7. He Y W, Guo C X, Pan Y F. 2008. Inhibition of hepatitis B virus replication by pokeweed antiviral protein. World J Gastroenterol, 14: 1592–1597.

    Article  PubMed  CAS  Google Scholar 

  8. Huang H, Chan H, Wang Y Y, et al. 2006. Trichosanthin suppress the elevation of p38 MAPK and Bcl-2 induced by HSV-1 infection in Vero cells. Life Sci, 13: 1287–1292.

    Article  Google Scholar 

  9. Huang P L, Sun Y, Chen H C, et al. 1999. Proteolytic fragments of anti-HIV and anti-tumor proteins MAP30 an GAP31 are biologically active. Biochem Biophys Res Commun, 262: 615–623.

    Article  PubMed  CAS  Google Scholar 

  10. Kahn J O, Gorelick K J, Gatti G, et al. 1994. Safety, activity and pharmacokinetics of GLQ223 in patients with AIDS and AIDS-related complex. Antimicrob Agents Ch, 38: 260–267.

    CAS  Google Scholar 

  11. Kaur I, Yadav SK, Puri M, et al. 2011. Balsamin, a novel ribosome inactivating protein from the seeds of Balsam apple Momordica balsamina. Amino Acids (accepted).

  12. Lee Huang S, Huang P L, Chen H C, et al. 1995a. Anti-HIV and anti-tumor activities of recombinant MAP30 from bitter melon. Gene, 161: 151–156.

    Article  PubMed  CAS  Google Scholar 

  13. Lee Huang S, Huang P L, Huang P L, et al. 1995b. Inhibition of the integrase of human immunodeficiency (HIV) type I by anti-HIV plant proteins MAP30 and GAP31. Proc Natl Acad Sci USA, 92: 8818–8822.

    Article  PubMed  CAS  Google Scholar 

  14. Lee Huang S, Huang P L, Nara P L, et al. 1990. MAP30: a new inhibitor of HIV-1 infection and replication. FEBS Lett, 272: 12–18.

    Article  PubMed  CAS  Google Scholar 

  15. Mansouri S, Choudhary G, Sarzala P M. 2009. Suppression of human T-cell leukemia virus I gene expression by pokeweed antiviral protein. J Biol Chem, 284: 31453–31462.

    Article  PubMed  CAS  Google Scholar 

  16. McGrath M S, Hwang K M, Cladwell S E, et al. 1989. GLQ223: an inhibitor of human immunodeficiency virus replication in acutely and chronically infected cells of lymphocyte and mononuclear phagocyte linege. Proc Natl Acad Sci USA, 86: 2844–2848.

    Article  PubMed  CAS  Google Scholar 

  17. Narouz N, Allan P S, Wade A H, et al. 2003. Genital herpes serptestinh: a study of the epidemiology and patient knowledge and attitude among STD clinic attnders in conventry, UK. Sex Transum Infect, 79: 35–41.

    Article  CAS  Google Scholar 

  18. Nugier F, Colin J N, Aymard M, et al. 1992. Occurrence and characterization of acyclovir-resistant herpes simplex virus isolates: report on a two-year sensitivity screening survey. J Med Virol, 36:1–12.

    Article  PubMed  CAS  Google Scholar 

  19. Peumans, W J, Hao Q, Van Damme E J M. 2001. Ribosome inactivating proteins from plants: more than RNA N-glycosidase? FASEB J, 15: 1493–1506.

    Article  PubMed  CAS  Google Scholar 

  20. Puri, M, Kaur I, Kanwar R K, et al. 2009 Ribosome inactivating protein (RIPs) from Momordica charantia for antiviral therapy. Curr Mol Med, 9: 1080–1094.

    Article  PubMed  CAS  Google Scholar 

  21. Puri, M. 2010. Biotechnological potential of Momordica balsamina revealed. Curr Pharma Biotechnol, 11, 229

    Article  CAS  Google Scholar 

  22. Rajamohan F, Venkatachalam T K, Irvin J D, et al. 1999. Pokeweed antiviral protein isoforms PAP-I, PAP-II and PAP-III depurinate RNA of human immunodeficiency virus (HIV-1). Biochem Biophys Res Commun, 260: 453–458.

    Article  PubMed  CAS  Google Scholar 

  23. Sawasaki T, Morishita R, Ozawa A, et al. 1999. Mechanism of ribosome RNA apurinic site specific lyase. Nucleic Acids Symp Ser, 42: 257–258.

    PubMed  CAS  Google Scholar 

  24. Stripe F, Battelli M G. 2006. Ribosome-inactivating proteins: progress and problems. Cell Mol Life Sci, 63: 1850–1866.

    Article  Google Scholar 

  25. Stripe F. 2004. Ribosome-inactivating proteins. Toxins, 44: 371–383.

    Google Scholar 

  26. Taylor G P, Matsuoka M. 2005. Natural history of adult T-cell leukemia/lymphoma and approaches to therapy. Oncogne, 24: 6047–6057.

    Article  CAS  Google Scholar 

  27. Uckun F M, Bellong K, O’ Neill K, et al. 1999. Toxicity, Biologcal activity and pharmacokinetic of TXU (Anti-CD)7-pokeweed antiviral protein in chimpanzees and adult patients infected with human immunodeficiency virus. J Pharmacol Exp Ther, 291: 1301–1307.

    PubMed  CAS  Google Scholar 

  28. Uckun F M, Chelstrom L M, Ahlgren L T, et al. 1998. TXU (Anti-CD7)-pokeweed antiviral protein as a potent inhibitor of human immunodeficiency virus. Antimicrob Agents Ch, 47: 1052–1061.

    Article  Google Scholar 

  29. Uckun F M, Rajamohan F, Pendergrass S, et al. 2003. Structure-based design and engineering of a non-toxic recombinant pokeweed antiviral protein with potent anti-human immunodeficiency virus activity. Antimicrob Agents Ch, 47: 1052–1061.

    Article  CAS  Google Scholar 

  30. Wang J H, Nie H L, Tam S C, et al. 2002. Anti-HIV-1 property of trichosanthin correlates with its ribosome inactivating activity. FEBS Lett, 531: 295–298.

    Article  PubMed  CAS  Google Scholar 

  31. Zarling J M, Moran P A, Haffar O, et al. 1990. Inhibition of HIV replication by pokeweed antiviral protein targeted to CD4+ cells by monoclonal antibodies. Nature, 347: 92–95.

    Article  PubMed  CAS  Google Scholar 

  32. Zhao W L, Feng D, Wu J, et al. 2010. Trichosanthin inhibits integration of human immunodeficiency virus type I trough depurinating the long-terminal repeats. Mol Biol Rep, 37:2093–2098.

    Article  PubMed  CAS  Google Scholar 

  33. Zhao W, Feng D, Sun S, et al. 2010. The aniviral protein of trichosanthin penetrates into human immunodeficiency virus type I. Acta Biochim Biophys Sin, 42: 91–97.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Centre for Biotechnology and Interdisciplinary Science (BioDeakin), Institute of Technology Research and Innovation (ITRI), Deakin University, Victoria, 3217, Australia

    Munish Puri

  2. Protein and Fermentation Biotechnology Laboratory, Department of Biotechnology, Punjabi University, Patiala, 147002, India

    Inderdeep Kaur & Munish Puri

  3. Department of Botany, Punjabi University, Patiala, 147002, India

    R. C. Gupta

Authors
  1. Inderdeep Kaur
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  2. R. C. Gupta
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  3. Munish Puri
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Correspondence to Munish Puri.

Additional information

Foundation item: Indo-Swiss Joint research Program (ISJRP) #17/2011

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Kaur, I., Gupta, R.C. & Puri, M. Ribosome inactivating proteins from plants inhibiting viruses. Virol. Sin. 26, 357–365 (2011). https://doi.org/10.1007/s12250-011-3223-8

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  • DOI: https://doi.org/10.1007/s12250-011-3223-8

Key words

  • Ribosome inactivating protein
  • Human immunodeficiency virus
  • Hepatitis B virus
  • Herpes simplex virus