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Bovine Herpesvirus 1 protein bICP0 represses the transcription of bISG15 in fetal bovine lung cells

Virologica Sinica volume 26, pages 403–408 (2011)Cite this article

Abstract

The ubiquitin-like modifier bISG15 is an antiviral protein found in fetal bovine lung (FBL) cells. Bovine Herpesvirus 1(BHV-1), which is a viral pathogen of cattle, can infect FBL cells and induce cytopathic effects. Real-time PCR assays showed that BHV-1’s infection could repress the basal or inducible transcription of bISG15 in FBL cells. It demonstrates that this repression effect depends on BHV-1 viral infection and new protein synthesis. Our previous work showed that bIRF-3 was the key factor in the stimulation of bISG15 in FBL cells, so the effect of BHV-1 viral protein on bIRF-3 activating the promoter of bISG15 was confirmed. The luciferase assay showed the BHV-1 viral protein bICP0 inhibited the activation of bISG15 promoter stimulated by bIRF-3. Taken together, our work suggested that BHV-1 had some molecular mechanism to resist the cellular bISG15’s antiviral functions.

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Authors and Affiliations

  1. Key Laboratory of Molecular Microbiology and Technology, Ministry of Education; Key Laboratory of Microbial Functional Genomics (Tianjin), Nankai University, Tianjin, 300071, China

    Chang Liu, Wen-tao Qiao & Yun-qi Geng

  2. School of Medicine, Nankai University, Tianjin, 300071, China

    Chang Liu & Xiao-hong Kong

Authors
  1. Chang Liu
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  2. Xiao-hong Kong
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  3. Wen-tao Qiao
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  4. Yun-qi Geng
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Corresponding author

Correspondence to Yun-qi Geng.

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Foundation items: National Natural Science Foundation of China (81071343) and (30970162).

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Liu, C., Kong, Xh., Qiao, Wt. et al. Bovine Herpesvirus 1 protein bICP0 represses the transcription of bISG15 in fetal bovine lung cells. Virol. Sin. 26, 403–408 (2011). https://doi.org/10.1007/s12250-011-3213-x

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  • DOI: https://doi.org/10.1007/s12250-011-3213-x

Key words

  • Herpesvirus
  • Cattle
  • Interferon
  • Innate immunity