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Molecular characterization of Banana streak virus isolate from Musa Acuminata in China

Virologica Sinica volume 26, pages 393–402 (2011)Cite this article

Abstract

Banana streak virus (BSV), a member of genus Badnavirus, is a causal agent of banana streak disease throughout the world. The genetic diversity of BSVs from different regions of banana plantations has previously been investigated, but there are relatively few reports of the genetic characteristic of episomal (non-integrated) BSV genomes isolated from China. Here, the complete genome, a total of 7722bp (GenBank accession number DQ092436), of an isolate of Banana streak virus (BSV) on cultivar Cavendish (BSAcYNV) in Yunnan, China was determined. The genome organises in the typical manner of badnaviruses. The intergenic region of genomic DNA contains a large stem-loop, which may contribute to the ribosome shift into the following open reading frames (ORFs). The coding region of BSAcYNV consists of three overlapping ORFs, ORF1 with a non-AUG start codon and ORF2 encoding two small proteins are individually involved in viral movement and ORF3 encodes a polyprotein. Besides the complete genome, a defective genome lacking the whole RNA leader region and a majority of ORF1 and which encompasses 6525bp was also isolated and sequenced from this BSV DNA reservoir in infected banana plants. Sequence analyses showed that BSAcYNV has closest similarity in terms of genome organization and the coding assignments with an BSV isolate from Vietnam (BSAcVNV). The corresponding coding regions shared identities of 88% and ∼95% at nucleotide and amino acid levels, respectively. Phylogenetic analysis also indicated BSAcYNV shared the closest geographical evolutionary relationship to BSAcVNV among sequenced banana streak badnaviruses.

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Authors and Affiliations

  1. Agricultural Ministry Key Laboratory of Tropical Crop Biotechnology, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China

    Jun Zhuang, Jian-hua Wang & Zhi-xin Liu

  2. Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China

    Jun Zhuang

  3. Environment and plant protection institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, 571734, China

    Xin Zhang

Authors
  1. Jun Zhuang
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  2. Jian-hua Wang
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  3. Xin Zhang
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  4. Zhi-xin Liu
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Corresponding author

Correspondence to Zhi-xin Liu.

Additional information

Foundation items: Nature Science Foundation of China (No. 30660100); National Key Technology R&D Program of China (2007BAD48B01); and National Nonprofit Institute Research Grant of CATAS-ITBB (ITBB110304).

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Zhuang, J., Wang, Jh., Zhang, X. et al. Molecular characterization of Banana streak virus isolate from Musa Acuminata in China. Virol. Sin. 26, 393–402 (2011). https://doi.org/10.1007/s12250-011-3212-y

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  • DOI: https://doi.org/10.1007/s12250-011-3212-y

Key words

  • BSAcYNV
  • Complete genome
  • Defective genome
  • Identity
  • Phylogenetic analysis