Crimean-Congo Haemorrhagic Fever Virus (CCHFV) is a tick-born virus of the Nairovirus genus within the Bunyaviridae family, which is widespread and causes high fatality. The nucleocapsid of CCHFV is comprised of N proteins that are encoded by the S segment. In this research, the N protein of CCHFV was expressed in insect cells using a recombinant baculovirus. Under an electron microscope, Virus-Like Particles (VLPs) with various size and morphology were observed in cytoplasmic vesicles in the infected cells. Sucrose-gradient purification of the cell lysate indicated that the VLPs were mainly located in the upper fraction after ultracentrifugation, which was confirmed by Western blot analysis and immuno-electron microscopy (IEM).
Barr J N, Wertz G W. 2004. Bunyamwera bunyavirus RNA synthesis requires cooperation of 3′- and 5′-terminal sequences. J Virol, 78: 1129–1138.
Chai J J, Xiao C E, Liu Y H, et al. 2004. Report of Investigation on Hemorrhagic Fever in Bachu County, Xinjiang in 1966 III. Investigation an Epidemiology III of Hemorrhagic Fever in Bachu. Endemic Disease Bulletin (in chinese), 19S: 15–22.
Chinikar S, Ghiasi S M, Hewson R, et al. 2010. Crimean-Congo hemorrhagic fever in Iran and neighboring countries. J Clin Virol, 47: 110–114.
Deng F, Wang R R, Fang M G, et al. 2007. Proteomics analysis of Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus identified two new occlusion-derived virus-associated proteins, HA44 and HA100. J Virol, 81: 9377–9385.
Deyde V M, Khristova M L, Rollin P E, et al. 2006. Crimean-Congo hemorrhagic fever virus genomics and global diversity. J Virol, 80: 8834–8842.
Elevli M, Ozkul A A, Civilibal M, et al. 2010. A newly identified Crimean-Congo hemorrhagic fever virus strain in Turkey. Int J Infect Dis, 14Suppl (3): e213–216.
Elliott R M. 1990. Molecular biology of the Bunyaviridae. J Gen Virol, 71: 501–522.
Ergonul O. 2006. Crimean-Congo haemorrhagic fever. Lancet Infect Dis, 6: 203–214.
Ergonul O. 2008. Treatment of Crimean-Congo hemorrhagic fever. Antiviral Res, 78: 125–131.
Flick R. 2007. Chapter 4: Molecular Biology of the Crimean-Congo Hemorrhagic Fever Virus. In: Crimean-Congo Hemorrhagic Fever (O Ergonul, C A Whitehouse ed.). Springer Netherlands, p 35–44.
Frank S A. 2002. Immunology and Evolution of Infectious Disease. Princeton (NJ): Pricenton University Press, p94–108.
Fu T M, Guan L, Friedman A, et al. 1999. Dose dependence of CTL precursor frequency induced by a DNA vaccine and correlation with protective immunity against influenza virus challenge. J Immunol, 162: 4163–4170.
Ghodake R S, Babar R S, Patil S B, et al. 2011. CCHFV-an emerging infectious disease. Pharma science monitor: An international journal of pharmaceutical sciences, 1067–1087.
Hewson R, Chamberlain J, Mioulet V, et al. 2004. Crimean-Congo haemorrhagic fever virus: sequence analysis of the small RNA segments from a collection of viruses world wide. Virus Res, 102: 185–189.
King L A, Possee R D. 1992. The baculovirus expression system: A laboratory guide. New York, NY (USA): Chapman & Hall. p158–159.
Kunkel M, Lorinczi M, Rijnbrand R, et al. 2001. Self-assembly of nucleocapsid-like particles from recombinant hepatitis C virus core protein. J Virol, 75: 2119–2129.
Lazo L, Gil L, Lopez C, et al. 2010. Nucleocapsid-like particles of dengue-2 virus enhance the immune response against a recombinant protein of dengue-4 virus. Arch Virol, 155: 1587–1595.
Liu L, Celma C C, Roy P. 2008. Rift Valley fever virus structural proteins: expression, characterization and assembly of recombinant proteins. Virol J, 5: 82.
Liu S J, Leng C H, Lien S P, et al. 2006. Immunological characterizations of the nucleocapsid protein based SARS vaccine candidates. Vaccine, 24: 3100–3108.
Lopez C, Gil L, Lazo L, et al. 2009. In vitro assembly of nucleocapsid-like particles from purified recombinant capsid protein of dengue-2 virus. Arch Virol, 154: 695–698.
Ma B J, Wang H. 2000. The hacker of mount Tianshan—Crimean-Congo hemorrhagic fever virus. Foreign medical Sciences (Section of Virology) (in chinese), 7: 8–10.
Midilli K, Gargılı A, Ergonul O, et al. 2007. Imported Crimean-Congo hemorrhagic fever cases in Istanbul. BMC Infectious Diseases, 7: 54.
Ozdarendeli A, Canakoglu N, Berber E, et al. 2010. The complete genome analysis of Crimean-Congo hemorrhagic fever virus isolated in Turkey. Virus Res, 147: 288–293.
Reguera J, Weber F, Cusack S. 2010. Bunyaviridae RNA polymerases (L-protein) have an N-terminal, influenza-like endonuclease domain, essential for viral cap-dependent transcription. PLoS Pathogens, 6: 1–14.
Saijo M, Qing T, Niikura M, et al. 2002. Recombinant nucleoprotein-based enzyme-linked immunosorbent assay for detection of immunoglobulin G antibodies to Crimean-Congo hemorrhagic fever virus. J Clin Microbiol, 40: 1587–1591.
Tang Q. 2006. Crimean-Congo hemorrhagic fever virus recently research progress. Chinese J Exp Clin Virol (in chinese), 20: 86–89.
Wang M L, Tan Y, Yin F F, et al. 2008. The F-like protein Ac23 enhances the infectivity of the budded virus of gp64-null Autographa californica multinucleocapsid nucleopolyhedrovirus pseudotyped with baculovirus envelope fusion protein F. J Virol, 82: 9800–9804.
Wang M L, Tuladhar E, Shen S, et al. 2010. Specificity of baculovirus P6.9 basic DNA-binding proteins and critical role of the C terminus in virion formation. J Virol, 84: 8821–8828.
Wang R l, Jin N Y, Zhao B, et al. 2005. Expression of the Gene Encoding the N Protein of SARS Virus in E.coli and Its Usd in the Immunization of Hourse. Virologica Sinica (in chinese), 20: 582–585.
Wasmoen T L, Kadakia N P, Unfer R C, et al. 1995. Protection of cats from infectious peritonitis by vaccination with a recombinant raccoon poxvirus expressing the nucleocapsid gene of feline infectious peritonitis virus. Adv Exp Med Biol, 380: 221–228.
Wei P F, Luo Y J, Li T X, et al. 2010. Serial expression of the truncated fragments of the nucleocapsid protein of CCHFV and identification of the epitope region. Virologica Sinica, 25: 45–51.
Whitehouse C A. 2004. Crimean-Congo hemorrhagic fever. Antiviral Res, 64: 145–160.
Wilson J A, Hart M K. 2001. Protection from Ebola virus mediated by cytotoxic T lymphocytes specific for the viral nucleoprotein. J Virol, 75: 2660–2664.
Xia H, Li P, Yang J, et al 2011. Epidemiological survey of Crimean-Congo hemorrhagic fever virus in Yunnan, China, 2008. Int J Infect Dis, 15: e459–463.
Foundation items: The Knowledge Innovation Program of the Chinese Academy of Sciences, Grant No. KSCX2-YW-N-065; the Knowledge Innovation Program of the Chinese Academy of Sciences, Grant No. KSCX2-EWG-8; National Key Basic Research Program (973 Program), Grant No. 2010CB530103; National Basic Research Priorities Program of China, Grant No. 2007 FY210700.
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Zhou, Zr., Wang, Ml., Deng, F. et al. Production of CCHF virus-like particle by a baculovirus-insect cell expression system. Virol. Sin. 26, 338–346 (2011). https://doi.org/10.1007/s12250-011-3209-6
- Crimean-Congo hemorrhagic fever virus (CCHFV)
- Virus-Like Particle (VLP)
- Nucleocapsid (N) protein