Abstract
Crimean-Congo Haemorrhagic Fever Virus (CCHFV) is a tick-born virus of the Nairovirus genus within the Bunyaviridae family, which is widespread and causes high fatality. The nucleocapsid of CCHFV is comprised of N proteins that are encoded by the S segment. In this research, the N protein of CCHFV was expressed in insect cells using a recombinant baculovirus. Under an electron microscope, Virus-Like Particles (VLPs) with various size and morphology were observed in cytoplasmic vesicles in the infected cells. Sucrose-gradient purification of the cell lysate indicated that the VLPs were mainly located in the upper fraction after ultracentrifugation, which was confirmed by Western blot analysis and immuno-electron microscopy (IEM).
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Foundation items: The Knowledge Innovation Program of the Chinese Academy of Sciences, Grant No. KSCX2-YW-N-065; the Knowledge Innovation Program of the Chinese Academy of Sciences, Grant No. KSCX2-EWG-8; National Key Basic Research Program (973 Program), Grant No. 2010CB530103; National Basic Research Priorities Program of China, Grant No. 2007 FY210700.
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Zhou, Zr., Wang, Ml., Deng, F. et al. Production of CCHF virus-like particle by a baculovirus-insect cell expression system. Virol. Sin. 26, 338–346 (2011). https://doi.org/10.1007/s12250-011-3209-6
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DOI: https://doi.org/10.1007/s12250-011-3209-6
Key words
- Crimean-Congo hemorrhagic fever virus (CCHFV)
- Virus-Like Particle (VLP)
- Nucleocapsid (N) protein