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Characterization and diagnostic use of a monoclonal antibody for VP28 envelope protein of white spot syndrome virus


The gene encoding the VP28 envelope protein of White spot syndrome virus (WSSV) was cloned into expression vector pET-30a and transformed into the Escherichia coli strain BL21. After induction, the recombinant VP28 (rVP28) protein was purified and then used to immunize Balb/c mice for monoclonal antibody (MAb) production. It was observed by immuno-electron microscopy the MAbs specific to rVP28 could recognize native VP28 target epitopes of WSSV and dot-blot analysis was used to detect natural WSSV infection. Competitive PCR showed that the viral level was approximately 104 copies/mg tissue in the dilution of gill homogenate of WSSV-infected crayfish at the detection limit of dot-blot assay. Our results suggest that dot-blot analysis with anti-rVP28 MAb could rapidly and sensitively detect WSSV at the early stages of WSSV infection.

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Correspondence to Hua-hua Du.

Additional information

Foundation item: NSFC (30901116); Zhejiang Provincial Natural Science Foundation of China (Y3080212); The Planned Science and Technology Project of Zhejiang Province, China (2008C32034).

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Hou, Cl., Cao, Y., Xie, Rh. et al. Characterization and diagnostic use of a monoclonal antibody for VP28 envelope protein of white spot syndrome virus. Virol. Sin. 26, 260–266 (2011).

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Key words

  • White spot syndrome virus (WSSV)
  • Recombinant VP28
  • Monoclonal antibody (MAb)