Skip to main content

Advertisement

SpringerLink
Log in

Epidemiological investigation and genome analysis of duck circovirus in Southern China

  • Published:
Virologica Sinica

Abstract

Duck circovirus (DuCV), a potential immunosuppressive virus, was investigated in Southern China from March 2006 to December 2009 by using a polymerase chain reaction (PCR) based method. In this study, a total of 138 sick or dead duck samples from 18 different farms were examined with an average DuCV infection rate of ∼35%. It was found that ducks between the ages of 40∼60 days were more susceptible to DuCV. There was no evidence showing that the DuCV virus was capable of vertical transmission. Farms with positive PCR results exhibited no regularly apparent clinical abnormalities such as feathering disorders, growth retardation or lower-than-average weight. The complete genomes of 9 strains from Fujian Province and 1 from Zhejiang Province were sequenced and analyzed. The 10 DuCV genomes, compared with others genomes downloaded from GenBank, ranged in size from 1988 to 1996 base pairs, with sequence identities ranging from 83.2% to 99.8%. Phylogenetic analysis based on genome sequences demonstrated that DuCVs can be divided into two distinct genetic genotypes, Group I (the Euro-USA lineage) and Group II (the Taiwan lineage), with approximately 10.0% genetic difference between the two types. Molecular epidemiological data suggest there is no obvious difference among DuCV strains isolated from different geographic locations or different species, including Duck, Muscovy duck, Mule duck, Cheery duck, Mulard duck and Pekin duck.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Banda A, Galloway-Haskins R I, Sandhu T S, et al. 2007. Genetic analysis of a duck circovirus detected in commercial Pekin ducks in New York. Avian Dis, 51: 90–95.

    Article  PubMed  Google Scholar 

  2. Chen C L, Wang P X, Lee M S, et al. 2006. Development of a polymerase chain reaction procedure for detection and differentiation of duck and goose circovirus. Avian Dis, 50: 92–95.

    Article  PubMed  Google Scholar 

  3. Eisenberg S W F, van Asten A J A M, van Ederen A M, et al. 2003. Detection of circovirus with a polymerase chain reaction in the ostrich (Struthio camelus) on a farm in The Netherlands. Vet Microbiol, 95: 27–38.

    Article  PubMed  CAS  Google Scholar 

  4. Faurez F, Dory D, Grasland B, et al. 2009. Replication of porcine circoviruses. Virol J, 18: 60.

    Article  Google Scholar 

  5. Fringuelli E, Scott AN, Beckett A, et al. 2005. Diagnosis of duck circovirus infections by conventional and real-time polymerase chain reaction tests. Avian Pathol, 34: 495–500.

    Article  PubMed  CAS  Google Scholar 

  6. Fu G, Cheng L, Shi S, et al. 2008. Genome Cloning and Sequence Analysis of Duck Circovirus. Chin J Virol, 24:138–143.(in Chinese)

    CAS  Google Scholar 

  7. Halami M Y, Nieper H, Müller H, et al. 2008. Detection of a novel circovirus in mute swans (Cygnus olor) by using nested broad-spectrum PCR. Virus Res, 132: 208–212.

    Article  PubMed  CAS  Google Scholar 

  8. Hattermann K, Schmitt C, Soike D, et al. 2003. Cloning and sequencing of Duck circovirus (DuCV). Arch Virol, 148: 2471–2480.

    Article  PubMed  CAS  Google Scholar 

  9. Hughes A L, Piontkivska H. 2008. Nucleotide sequence polymorphism in circoviruses. Infect Genet Evol, 8: 130–138.

    Article  PubMed  CAS  Google Scholar 

  10. Jiang S, Zhang X, Liu S, et al. 2008. PCR detection and sequence Analysis of duck circovirus in sick Muscovy ducks. Virol Sin, 23: 265–271.

    Article  CAS  Google Scholar 

  11. Johne R, Fernández-de-Luco D, Höfle U, et al. 2006. Genome of a novel circovirus of starlings, amplified by multiply primed rolling-circle amplification. J Gen Virol, 87: 1189–1195.

    Article  PubMed  CAS  Google Scholar 

  12. Li M, Song Y, Wen N, et al. 2009. PCR detection and sequence analysis of duck circovirus infection in Xinxing County of Guangdong Province. Chin Vet Sci, 39: 164–167. (in Chinese)

    CAS  Google Scholar 

  13. Mankertz A, Hattermann K, Ehlers B, et al. 2000. Cloning and sequencing of columbid circovirus (CoCV), a new circovirus from pigeons. Arch Virol, 145: 2469–2479.

    Article  PubMed  CAS  Google Scholar 

  14. Pheix K V, Weston J H, Ypelaar I, et al. 2001. Nucleotide sequence analysis of a novel circvirus of canaries and its relationship to other members of the genus Circovirus of the family Circoviridae. J Gen Virol, 82: 2805–2809.

    Google Scholar 

  15. Rahaus M, Dwsloges N, Probst S, et al. 2008. Detection of beak and feather disease virus DNA in embryonated eggs of psittacine birds. Vet Med-Czech, 53: 53–58.

    CAS  Google Scholar 

  16. Raidal S R, Riddoch P A. 1997. A feather disease in Senegal doves (Streptopelia senegalensis) morphologically similar to psittacine beak and feather disease. Avian Pathol, 26: 829–836.

    Article  PubMed  CAS  Google Scholar 

  17. Schat K A. 2009. Chicken anemia virus. Curr Top Microbiol Immunol, 331: 151–183.

    Article  PubMed  CAS  Google Scholar 

  18. Shi S, Chen Z, Yang W, et al. 2009. Genomic sequence analysis of duck circovirus and prokaryotic expression of its C1 truncated gene. Chin J Vet Sci, 29: 1269–1273. (in Chinese)

    CAS  Google Scholar 

  19. Shi S, Fu G, Cheng L, et al. 2010. Sequencing and analysis of genome of duck circovirus PT07. Chin J Prev Vet Med, 32: 235–237. (in Chinese)

    CAS  Google Scholar 

  20. Soike D, Albrecht K, Hattermann K, et al. 2004. Novel circovirus in mulard ducks with developmental and feathering disorders. Vet Rec, 154: 792–793.

    Article  PubMed  CAS  Google Scholar 

  21. Soike D, Kohler B, Albrecht K. 1999. A circovirus-like infection in geese related to a runting syndrome. Avian Pathol, 28: 199–202.

    Article  Google Scholar 

  22. Stewart M E, Perry R, Raidal S R. 2006. Identification of a novel circovirus in Australian ravens (Corvus coronoides) with feather disease. Avian Pathol, 35: 86–92.

    Article  PubMed  CAS  Google Scholar 

  23. Tamura K, Dudley J, Nei M, et al. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol, 24: 1596–1599.

    Article  PubMed  CAS  Google Scholar 

  24. Todd D, Fringuelli E, Scott A N, et al. 2008. Sequence comparison of pigeon circoviruses. Res Vet Sci, 84: 311–319.

    Article  PubMed  CAS  Google Scholar 

  25. Todd D, Scott A N, Fringuelli E, et al. 2007. Molecular characterization of novel circoviruses from finch and gull. Avian Pathol, 36: 75–81.

    Article  PubMed  CAS  Google Scholar 

  26. Todd D, Weston J, Ball N W, et al. 2001. Nucleotide sequence-based identification of a novel circovirus of canaries. Avian Pathol, 30: 321–325.

    Article  PubMed  CAS  Google Scholar 

  27. Todd D, Weston J H, Soike D, et al. 2001. Genome sequence determinations and analyses of novel circoviruses from goose and pigeon. Virology, 286: 354–362.

    Article  PubMed  CAS  Google Scholar 

  28. Todd D. 2004. Avian circovirus diseases. Lessons for the study of PMWS. Vet Microbiol, 98: 169–174.

    Article  PubMed  CAS  Google Scholar 

  29. Yu X, Zhu C, Zheng X, et al. 2007. Genome analysis and epidemiological investigation of goose circovirus detected in eastern China. Virus Genes, 35. 605–609.

    Article  PubMed  CAS  Google Scholar 

  30. Zhang X, Jiang S, Wu J, et al. 2009. An investigation of duck circovirus and co-infection in Cherry Valley ducks in Shandong Province, China. Vet Microbiol, 133: 252–256.

    Article  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agricultural Sciences, Fuzhou, 350013, China

    Chun-he Wan, Guang-hua Fu, Shao-hua Shi, Long-fei Cheng, Hong-mei Chen, Chun-xiang Peng, Su Lin & Yu Huang

Authors
  1. Chun-he Wan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Guang-hua Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shao-hua Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Long-fei Cheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Hong-mei Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Chun-xiang Peng
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Su Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Yu Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yu Huang.

Additional information

Foundation items: Modern Agri-industry Technology Research System (CARS-43); Science and Technology Innovation Fund of Fujian Academy of Agriculture Science (STIF-02); Science and Technology Innovation Foundation for Young Scientists of Fujian Academy of Agriculture Science (2008QB-6).

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wan, Ch., Fu, Gh., Shi, Sh. et al. Epidemiological investigation and genome analysis of duck circovirus in Southern China. Virol. Sin. 26, 289–296 (2011). https://doi.org/10.1007/s12250-011-3192-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12250-011-3192-y

Key words

Search

Navigation