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Genotyping of white spot syndrome virus in Chinese cultured shrimp during 1998–1999

Virologica Sinica volume 26, pages 123–130 (2011)Cite this article

Abstract

Recent studies showed that white spot syndrome virus (WSSV) isolates from different geographic locations share a high genetic similarity except the variable regions in ORF23/24 and ORF14/15, and variable number of tandem repeats (VNTR) within ORF94. In this study, genotyping was performed according to these three variable regions among WSSV isolates collected during 1998/1999 from Southern China. These WSSV isolates contain a deletion of 1168, 5657, 5898, 9316 and 11093 bp, respectively in the variable region ORF23/24 compared with WSSV-TW, and a deletion of 4749 or 5622 bp in the variable region ORF14/15 relative to TH-96-II. Four types of repeat units (RUs) (6, 8, 9 and 13 RUs) in ORF94 were detected in these isolates, with the shortest 6 RUs as the most prevalent type. Our results provide important information for a better understanding of the spatio-temporal transmission mode and the WSSV genetic evolution lineage.

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Author notes

  1. Yan-wei Tan

    Present address: Institute of Neurobiology, Im Neuenheimer Feld 364, University of Heidelberg, 69120, Heidelberg, Germany

Authors and Affiliations

  1. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China

    Yan-wei Tan & Zheng-li Shi

Authors
  1. Yan-wei Tan
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  2. Zheng-li Shi
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Correspondence to Zheng-li Shi.

Additional information

Foundation item: The State Key Program for Basic Research Grants (2006CB101801)

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Tan, Yw., Shi, Zl. Genotyping of white spot syndrome virus in Chinese cultured shrimp during 1998–1999. Virol. Sin. 26, 123–130 (2011). https://doi.org/10.1007/s12250-011-3181-1

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  • DOI: https://doi.org/10.1007/s12250-011-3181-1

Key words

  • WSSV
  • Genotyping
  • Variable region ORF23/24
  • ORF14/15
  • ORF94, China