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Virologica Sinica

, Volume 26, Issue 2, pp 123–130 | Cite as

Genotyping of white spot syndrome virus in Chinese cultured shrimp during 1998–1999

  • Yan-wei Tan
  • Zheng-li Shi
Article

Abstract

Recent studies showed that white spot syndrome virus (WSSV) isolates from different geographic locations share a high genetic similarity except the variable regions in ORF23/24 and ORF14/15, and variable number of tandem repeats (VNTR) within ORF94. In this study, genotyping was performed according to these three variable regions among WSSV isolates collected during 1998/1999 from Southern China. These WSSV isolates contain a deletion of 1168, 5657, 5898, 9316 and 11093 bp, respectively in the variable region ORF23/24 compared with WSSV-TW, and a deletion of 4749 or 5622 bp in the variable region ORF14/15 relative to TH-96-II. Four types of repeat units (RUs) (6, 8, 9 and 13 RUs) in ORF94 were detected in these isolates, with the shortest 6 RUs as the most prevalent type. Our results provide important information for a better understanding of the spatio-temporal transmission mode and the WSSV genetic evolution lineage.

Key words

WSSV Genotyping Variable region ORF23/24 ORF14/15 ORF94, China 

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References

  1. 1.
    Benson G. 1999. Tandem repeats finder:a program to analyze DNA sequences. Nucleic Acids Res, 27: 573–580.CrossRefPubMedGoogle Scholar
  2. 2.
    Dieu B T, Marks H, Siebenga J J, et al. 2004. Molecular epidemiology of white spot syndrome virus within Vietnam. J Gen Virol, 85: 3607–3618.CrossRefPubMedGoogle Scholar
  3. 3.
    Durand S, Lightner D V, Redman R M, et al. 1997. Ultrastructure and morphogenesis of White spot syndrome baculovirus (WSSV). Dis Aquat Organ, 28: 205–211.CrossRefGoogle Scholar
  4. 4.
    Escobedo-Bonilla C M, Alday-Sanz V, Wille M, et al. 2008. A review on the morphology, molecular characterization, morphogenesis and pathogenesis of white spot syndrome virus. J Fish Dis, 31: 1–18.CrossRefPubMedGoogle Scholar
  5. 5.
    Hoa T T T, Hodgson R A J, Oanh D T H, et al. 2005. Genotypic variations in tandem repeat DNA segments between ribonucleotide reductase subunit genes of white spot syndrome virus (WSSV) isolates from Vietnam. In: Diseases in Asian Aquaculture V, Fish Health Section (Walker P, Lester R, Bondad-Reantaso, ed.), Asian Fisheries Society, Manila (2005): 339–351.Google Scholar
  6. 6.
    Lan Y, Lu W, Xu X. 2002. Genomic instability of prawn white spot bacilliform virus (WSBV) and its association to virus virulence. Virus Res, 90: 269–274.CrossRefPubMedGoogle Scholar
  7. 7.
    Lightner DV. 1996. A handbook of pathology and diagnostic procedures for diseases of penaeid shrimp. In Special publication of the World Aquaculture Society LA: Baton RougeGoogle Scholar
  8. 8.
    Marks H, Goldbach R W, Vlak J M, et al. 2004. Genetic variation among isolates of White spot syndrome virus. Arch Virol, 149: 673–697.CrossRefPubMedGoogle Scholar
  9. 9.
    Marks H, van Duijse J J, Zuidema D, et al. 2005. Fitness and virulence of an ancestral White Spot Syndrome Virus isolate from shrimp. Virus Res, 110: 9–20.CrossRefPubMedGoogle Scholar
  10. 10.
    Mayo M A. 2002. A summary of taxonomic changes recently approved by ICTV. Arch Virol, 147: 1655–1663.CrossRefPubMedGoogle Scholar
  11. 11.
    Musthaqa S S, Sudhakarana R, Ishaq Ahmeda V P, et al. 2006. Variability in the tandem repetitive DNA sequences of white spot syndrome virus (WSSV) genome and suitability of VP28 gene to detect different isolates of WSSV from India. Aquaculture, 256: 34–41.CrossRefGoogle Scholar
  12. 12.
    Pradeep B, Shekar M, Gudkovs N, et al. 2008. Genotyping of white spot syndrome virus prevalent in shrimp farms of India. Dis Aquat Organ, 78: 189–198.CrossRefPubMedGoogle Scholar
  13. 13.
    Pradeep B, Shekar M, Karunasagar I, et al. 2008. Characterization of variable genomic regions of Indian white spot syndrome virus. Virology, 376: 24–30.CrossRefPubMedGoogle Scholar
  14. 14.
    Tan Y, Xing Y, Zhang H, et al. 2009. Molecular detection of three shrimp viruses and genetic variation of white spot syndrome virus in Hainan Province, China, in 2007. J Fish Dis, 32: 777–784.CrossRefPubMedGoogle Scholar
  15. 15.
    Thompson J D, Higgins D G, Gibson T J. 1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res, 22: 4673–4680.CrossRefPubMedGoogle Scholar
  16. 16.
    van Hulten M C, Witteveldt J, Peters S, et al. 2001. The white spot syndrome virus DNA genome sequence. Virology, 286: 7–22.CrossRefPubMedGoogle Scholar
  17. 17.
    Vlak J M, Bonami J R, Flegel T W, et al. 2005. Nimaviridae. In VIIIth Report of the International Committee on Taxonomy of Viruses. Edited by C M Fauquet, M A Mayo, J Maniloff, U Desselberger & L A Ball: Elsevier pp: 187–192.Google Scholar
  18. 18.
    Wang C H, Lo C F, Leu J H, et al. 1995. Purification and genomic analysis of baculovirus associated with White spot syndrome (WSBV) of Penaeus monodon. Dis Aquat Org, 23: 239–242.CrossRefGoogle Scholar
  19. 19.
    Wongteerasupaya C, Pungchai P, Withyachumnarnkul B, et al. 2003. High variation in repetitive DNA fragment length for white spot syndrome virus (WSSV) isolates in Thailand. Dis Aquat Organ, 54: 253–257.CrossRefPubMedGoogle Scholar
  20. 20.
    Yang F, He J, Lin X, et al. 2001. Complete genome sequence of the shrimp white spot bacilliform virus. J Virol, 75: 11811–11820.CrossRefPubMedGoogle Scholar

Copyright information

© Wuhan Institute of Virology, CAS and Springer-Verlag Berlin Heidelberg 2011

Authors and Affiliations

  1. 1.State Key Laboratory of Virology, Wuhan Institute of VirologyChinese Academy of SciencesWuhanChina

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