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Host cell protein C9orf69 promotes viral proliferation via interaction with HSV-1 UL25 protein

Virologica Sinica volume 26, pages 171–180 (2011)Cite this article

Abstract

In light of the scarcity of reports on the interaction between HSV-1 nucleocapsid protein UL25 and its host cell proteins, the purpose of this study is to use yeast two-hybrid screening to search for cellular proteins that can interact with the UL25 protein. C9orf69, a protein of unknown function was identified. The interaction between the two proteins under physiological conditions was also confirmed by biological experiments including co-localization by fluorescence and immunoprecipitation. A preliminary study of the function of C9orf69 showed that it promotes viral proliferation. Further studies showed that C9orf69 did not influence viral multiplication efficiency by transcriptional regulation of viral genes, but indirectly promoted proliferation via interaction with UL25.

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Authors and Affiliations

  1. Institute of Medical Biology, Chinese Academy of Medicine Science & Peking Union Medical College, Kunming, 650118, China

    Ying Zhang, Yan-mei Li, Long-ding Liu, Li Jiang, Ma Ji, Rui-ju Jiang, Lei Guo, Yun Liao & Qi-han Li

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  1. Ying Zhang
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  2. Yan-mei Li
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  3. Long-ding Liu
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  4. Li Jiang
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  5. Ma Ji
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  6. Rui-ju Jiang
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  7. Lei Guo
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  8. Yun Liao
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  9. Qi-han Li
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Correspondence to Qi-han Li.

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Foundation item: Scientific Research Fund of the Institute of Pathogen Biology (2007IPB10)

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Zhang, Y., Li, Ym., Liu, Ld. et al. Host cell protein C9orf69 promotes viral proliferation via interaction with HSV-1 UL25 protein. Virol. Sin. 26, 171–180 (2011). https://doi.org/10.1007/s12250-011-3179-8

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  • DOI: https://doi.org/10.1007/s12250-011-3179-8

Key words

  • Herpes simplex virus (HSV-1)
  • UL25
  • C9orf69
  • Proliferation