Inducing animal viruses to adapt to chicken embryos or chicken embryo fibroblasts (CEF) is a common method to develop attenuated live vaccines with full security. Canine distemper virus (CDV) also does this, but the mechanisms and particular receptors remain unclear. Virus overlay protein blot assays were carried out on CEF membrane proteins, which were extracted respectively with a Mem-PER™ kit, a radioimmunoprecipitation assay buffer or a modified co-immunoprecipitation method, and revealed a common 57 kDa positive band that differed from the 42-kDa positive band in Vero cells and also from those receptors reported in lymphocytes and 293 cells, indicating a receptor diversity of CDV and the possibility of the 57-kDa protein acting as a receptor that is involved in adaptive infection of CDV Kunming strain to CEF.
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Fundation items: Yunnan Provincial Education Board (08C0070); Yunnan Provincial Program for Introducing High-level Scientists (2009CI125).
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Chen, J., Liang, X. & Chen, Pf. Canine distemper virus utilizes different receptors to infect chicken embryo fibroblasts and vero cells. Virol. Sin. 26, 139–145 (2011). https://doi.org/10.1007/s12250-011-3176-3
- Canine distemper virus (CDV)
- Cellular receptor
- Chicken embryo fibroblasts (CEF)
- Vero cells
- Virus overlay protein blot assay