Southern rice black-streaked dwarf virus (SRBSDV) is a novel Fijivirus prevalent in rice in southern and central China, and northern Vietnam. Its genome has 10 segments of double-stranded RNA named S1 to S10 according to their size. An isolate of SRBSDV, JNi4, was obtained from naturally infected maize plants from Ji’ning, Shandong province, in the 2008 maize season. Segments S7 to S10 of JNi4 share nucleotide identities of 72.6%–73.1%, 72.3%–73%, 73.9%–74.5% and 77.3%–79%, respectively, with corresponding segments of Rice black-streaked dwarf virus isolates, and identities of 99.7%, 99.1%–99.7%, 98.9%–99.5%, and 98.6%–99.2% with those of SRBSDV isolates HN and GD. JNi4 forms a separate branch with GD and HN in the phylogenetic trees constructed with genomic sequences of S7 to S10. These results confirm the proposed taxonomic status of SRBSDV as a distinct species of the genus Fijivirus and indicate that JNi4 is an isolate of SRBSDV. Shandong is so far the northernmost region where SRBSDV is found in China.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
Anzola J V, Xu Z K, Asamizu T, et al. 1987. Segment-specific inverted repeats found adjacent to conserved terminal sequences in wound tumor virus genome and defective interfering RNAs. Proc Nat Acad Sci USA, 84: 8301–8305.
Bao Y X, Xu X Y, Wang J Q, et al. 2007. Analysis of the atmospheric dynamical backgrounds for the great immigration events of white back planthoppper (Sogatella furcifera). Acta Ecol Sin, 27: 4527–4535.
Chen J, Zhu Q Q, Yuan C Y, et al. 2008. Molecular characterization of Rice black-streaked dwarf virus isolates causing maize rough dwarf disease in Shandong. Acta Phytopathol Sin, 38: 540–543.
Distéfano A J, Conci L R, Muñoz Hidalgo M, et al. 2002. Sequence analysis of genome segments S4 and S8 of Mal de Río Cuarto virus (MRCV): evidence that the virus should be a separate Fijivirus species. Arch Virol, 147: 1699–1709.
Distefano A J, Hopp H E, del Vas M. 2005. Sequence analysis of genome segments S5 and S10 of Mal de Rio Cuarto virus (Fijivirus, Reoviridae). Arch Virol, 150: 1241–1248.
Fang S, Yu J, Feng J, et al. 2001. Identification of rice black-streaked dwarf fijivirus in maize with rough dwarf disease in China. Arch Virol, 146: 167–170.
Guzman F A, Distefano A J, Arneodo J D, et al. 2007. Sequencing of the bicistronic genome segments S7 and S9 of Mal de Rio Cuarto virus (Fijivirus, Reoviridae) completes the genome of this virus. Arch Virol, 152: 565–573.
Harding R M, Burns P, Geijskes R J, et al. 2006. Molecular analysis of Fiji disease virus segments 2, 4 and 7 completes the genome sequence. Virus Genes, 32: 43–47.
Isogai M, Uyeda I, Lindsten K. 1998. Taxonomic characteristics of fijiviruses based on nucleotide sequences of the oat sterile dwarf virus genome. J Gen Virol, 79: 1479–1485.
Kumar S, Nei M, Dudley J, et al. 2008. MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. Brief Bioinform, 9: 299–306.
Larkin M A, Blackshields G, Brown N P, et al. 2007. Clustal W and Clustal X version 2.0. Bioinformatics, 23: 2947–2948.
Marzachì C, Antoniazzi S, d’Aquilio M, et al. 1996. The double-stranded RNA genome of maize rough dwarf fijivirus contains both mono and dicistronic segments. Eur J Plant Pathol, 102: 601–605.
Marzachi C, Boccardo G, Nuss D L. 1991. Cloning of the maize rough dwarf virus genome: molecular confirmation of the plant-reovirus classification scheme and identification of two large nonoverlapping coding domains within a single genomic segment. Virology, 180: 518–526.
McQualter R B, Burns P, Smith G R, et al. 2004. Molecular analysis of Fiji disease virus genome segments 5, 6, 8 and 10. Arch Virol, 149: 713–721.
Milne R G, del Vas M, Harding R M, et al. 2005. Genus Fijivirus. in: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Virus Taxomony: Classification and nomenclature of viruses. Eighth Report of the International Committee on the Taxonomy of Viruses. Elsevier Academic Press, San Diego, pp 534–542.
Nakashima N, Koizumi M, Watanabe H, et al. 1996. Complete nucleotide sequence of the Nilaparvata lugens reovirus: a putative member of the genus Fijivirus. J Gen Virol, 77: 139–146.
Nakashima N, Noda H. 1994. Nucleotide sequence of Nilaparvata lugens reovirus genome segment S8 coding for the major outer capsid protein. J Gen Virol, 75: 2803–2806.
Noda H, Nakashima N, Omura T. 1994. Cloning of the Nilaparvata lugens reovirus genome: conserved terminal nucleotide sequences and nucleotide sequence of genome segment S10. J Gen Virol, 75: 221–225.
Soo H M, Handley J A, Maugeri M M, et al. 1998. Molecular characterization of Fiji disease fijivirus genome segment 9. J Gen Virol, 79: 3155–61.
Tamura K, Dudley J, Nei M, et al. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol, 24: 1596–1599.
Thompson J D, Higgins D G, Gibson T J. 1994. improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucl Acids Res, 22: 4673–4680.
Wang Q, Yang J, Zhou G H, et al. 2010. The complete genome sequence of two isolates of southern rice black-streaked dwarf virus, a new member of the genus Fijivirus. J Phytopathol, 158: 733–737
Wang Z H, Fang S G, Xu J L, et al. 2003. Sequence analysis of the complete genome of rice black-streaked dwarf virus isolated from maize with rough dwarf disease. Virus Genes, 27: 163–168.
Yu X Q, Lan Y F, Wang H Y, et al. 2007. The complete genomic sequence of Tobacco vein banding mosaic virus and its similarities with other potyviruses. Virus Genes, 35: 801–806.
Zhang H M, Chen J P, Adams M J. 2001. Molecular characterisation of segments 1 to 6 of Rice black-streaked dwarf virus from China provides the complete genome. Arch Virol, 146: 2331–2339.
Zhang H M, Chen J P, Lei J L, et al. 2001. Sequence analysis shows that a dwarfing disease on rice, wheat and maize in China is caused by rice black-streaked dwarf virus. Eur J Plant Pathol, 107: 563–567.
Zhang H M, Yang J, Chen J P, et al. 2008. A black-streaked dwarf disease on rice in China is caused by a novel fijivirus. Arch Virol, 153: 1893–1898.
Zhou G H, Wen J J, Cai D J, et al. 2008. Southern rice black-streaked dwarf virus: A new proposed Fijivirus species in the family Reoviridae. Chin Sci Bull, 53: 3677–3685.
Foundation items: National Natural Science Foundation of China (30971895, 31011130031); Special Research Funds for the Doctoral Program of Higher Education (20080434006); Grants from Ministry of Science and Technology (2009ZX08003-014B) and Shandong province (2009GG10009021); Modern maize industrial system of Shandong province.
These authors contributed equally.
Rights and permissions
About this article
Cite this article
Yin, X., Xu, Ff., Zheng, Fq. et al. Molecular characterization of segments S7 to S10 of a southern rice black-streaked dwarf virus isolate from maize in northern China. Virol. Sin. 26, 47–53 (2011). https://doi.org/10.1007/s12250-011-3170-9
- Southern rice black-streaked dwarf virus (SRBSDV)
- Maize rough dwarf disease (MRD)
- Phylogenetic analysis