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Effect of siRNAs on HSV-1 plaque formation and relative expression levels of RR mRNA

Virologica Sinica volume 26, pages 40–46 (2011)Cite this article

Abstract

RNA interference (RNAi) is a process by which introduced small interfering RNA (siRNA) can cause the specific degradation of mRNA with identical sequences. The human herpes simplex virus type 1 (HSV-1) RR is composed of two distinct homodimeric subunits encoded by UL39 and UL40, respectively. In this study, we applied siRNAs targeting the UL39 and UL40 genes of HSV-1. We showed that synthetic siRNA silenced effectively and specifically UL39 and UL40 mRNA expression and inhibited HSV-1 replication. Our work offers new possibilities for RNAi as a genetic tool for inhibition of HSV-1 replication.

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Authors and Affiliations

  1. Biomedicine research and development center of Jinan University, Guangdong, Guangzhou, 510632, China

    Zhe Ren, Shen Li, Qiao-li Wang, Yang-fei Xiang, Yun-xia Cui & Yi-fei Wang

  2. Medical College of Jinan University, Guangdong, Guangzhou, 510632, China

    Zhe Ren, Ren-bin Qi & Da-xiang Lu

  3. National Engineering Research Center of Genetic Medicine, Guangdong, Guangzhou, 510632, China

    Shen Li, Qiao-li Wang, Yang-fei Xiang, Yun-xia Cui & Yi-fei Wang

  4. National Institute for the Control of Pharmaceutical and Biological Products, Beijing, 100050, China

    Shu-min Zhang

  5. Shanghai GenePharma Co., Ltd, Shanghai, 201203, China

    Pei-zhuo Zhang

Authors
  1. Zhe Ren
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  2. Shen Li
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  3. Qiao-li Wang
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  8. Da-xiang Lu
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  9. Shu-min Zhang
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  10. Pei-zhuo Zhang
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Corresponding authors

Correspondence to Yi-fei Wang, Da-xiang Lu or Shu-min Zhang.

Additional information

These authors contributed equally to this work.

Foundation items: The Nation “863” Program of China (2006AA02A226); The Joint Funds of National Science Foundation of China (U0632010); The State Key Laboratory of Phytochemistry and Plant Resources in West China; Chinese Academy of Sciences (O807 B11211, O807E21211) and “211 grant of MOE”.

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Ren, Z., Li, S., Wang, Ql. et al. Effect of siRNAs on HSV-1 plaque formation and relative expression levels of RR mRNA. Virol. Sin. 26, 40–46 (2011). https://doi.org/10.1007/s12250-011-3162-9

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  • DOI: https://doi.org/10.1007/s12250-011-3162-9

Key words

  • Small interfering RNA (siRNA)
  • Herpes simplex virus type I (HSV-1)
  • Ribonucleotide reductase (RR)
  • Gene