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Development of a polyclonal antibody-based AC-ELISA and its comparison with PCR for diagnosis of canine parvovirus infection
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  • Published: 08 October 2010

Development of a polyclonal antibody-based AC-ELISA and its comparison with PCR for diagnosis of canine parvovirus infection

  • Manoj Kumar1,
  • Sukdeb Nandi1 &
  • Sunil Chidri1 

Virologica Sinica volume 25, pages 352–360 (2010)Cite this article

  • 651 Accesses

  • 10 Citations

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Abstract

A polyclonal antibody-based antigen-capture ELISA (AC-ELISA) has been developed for detection of Canine parvovirus (CPV) antigens in faecal samples of dogs. The assay uses rabbit anti-CPV polyclonal antibody as the capture antibody, guinea pig anti-CPV polyclonal antibody as tracing antibody and anti-guinea pig HRPO conjugate as the detection system. The optimum dilution of the capture antibody and the tracing antibody capable of detecting the CPV-2 antigens was found to be 1:1 600 and 1:400, respectively, in the check-board titration. In this study, a total of 152 samples (129 faecal samples and 23 cell culture supernatant) were tested both by AC-ELISA and by polymerase chain reaction (PCR). Of the samples tested, 69 and 78 samples were found positive by AC-ELISA and PCR, respectively. The AC-ELISA had relative sensitivity, relative specificity and accuracy of 88.4%, 100.0% and 91.4% respectively. The analytical sensitivity of AC-ELISA was estimated to be 102.8 TCID50/mL whereas PCR sensitivity was 100.8 TCID50/mL. The AC-ELISA is a simple, quick and reliable method for screening large numbers of faecal samples of dogs suspected of CPV infection.

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Authors and Affiliations

  1. Virology Laboratory, Centre for Animal Disease Research and Diagnosis, Indian Veterinary Research Institute, Izatnagar, Bareilly, U.P., 243122, India

    Manoj Kumar, Sukdeb Nandi & Sunil Chidri

Authors
  1. Manoj Kumar
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  2. Sukdeb Nandi
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  3. Sunil Chidri
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Corresponding author

Correspondence to Sukdeb Nandi.

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Kumar, M., Nandi, S. & Chidri, S. Development of a polyclonal antibody-based AC-ELISA and its comparison with PCR for diagnosis of canine parvovirus infection. Virol. Sin. 25, 352–360 (2010). https://doi.org/10.1007/s12250-010-3132-x

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  • Received: 15 March 2010

  • Accepted: 09 June 2010

  • Published: 08 October 2010

  • Issue Date: October 2010

  • DOI: https://doi.org/10.1007/s12250-010-3132-x

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Key words

  • Canine parvovirus (CPV)
  • Polyclonal antibody
  • Antigen-capture ELISA(AC-ELISA)
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