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Expression and immunological analysis of capsid protein precursor of swine vesicular disease virus HK/70

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Virologica Sinica

Abstract

VP1, a capsid protein of swine vesicular disease virus, was cloned from the SVDV HK/70 strain and inserted into retroviral vector pBABE puro, and expressed in PK15 cells by an retroviral expression system. The ability of the VP1 protein to induce an immune response was then evaluated in guinea pigs. Western blot and ELISA results indicated that the VP1 protein can be recognized by SVDV positive serum, Furthermore, anti-SVDV specific antibodies and lymphocyte proliferation were elicited and increased by VP1 protein after vaccination. These results encourage further work towards the development of a vaccine against SVDV infection.

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Authors and Affiliations

  1. Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Lanzhou, 730046, China

    Hong Tian, Jing-yan Wu, You-jun Shang, Shuang-hui Ying, Hai-xue Zheng & Xiang-tao Liu

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  1. Hong Tian
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  2. Jing-yan Wu
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  3. You-jun Shang
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  4. Shuang-hui Ying
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  5. Hai-xue Zheng
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  6. Xiang-tao Liu
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Correspondence to Xiang-tao Liu.

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Tian, H., Wu, Jy., Shang, Yj. et al. Expression and immunological analysis of capsid protein precursor of swine vesicular disease virus HK/70. Virol. Sin. 25, 206–212 (2010). https://doi.org/10.1007/s12250-010-3100-x

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  • DOI: https://doi.org/10.1007/s12250-010-3100-x

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