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Specificity of developmental resistance in gypsy moth (Lymantria dispar) to two DNA-insect viruses

Virologica Sinica volume 24, pages 493–500 (2009)Cite this article

Abstract

Gypsy moth (Lymantria dispar) larvae displayed marked developmental resistance within an instar to L. dispar M nucleopolyhedrovirus (LdMNPV) regardless of the route of infection (oral or intrahemocoelic) in a previous study, indicating that in gypsy moth, this resistance has a systemic component. In this study, gypsy moth larvae challenged with the Amsacta moorei entomopoxvirus (AMEV) showed developmental resistance within the fourth instar to oral, but not intrahemocoelic, inoculation. In general, gypsy moth is considered refractory to oral challenge with AMEV, but in this study, 43% mortality occurred in newly molted fourth instars fed a dose of 5×106 large spheroids of AMEV; large spheroids were found to be more infectious than small spheroids when separated by a sucrose gradient. Developmental resistance within the fourth instar was reflected by a 2-fold reduction in mortality (18%–21%) with 5×106 large spheroids in larvae orally challenged at 24, 48 or 72 h post-molt. Fourth instars were highly sensitive to intrahemocoelic challenge with AMEV; 1PFU produced approximately 80% mortality regardless of age within the instar. These results indicate that in gypsy moth, systemic developmental resistance may be specific to LdMNPV, reflecting a co-evolutionary relationship between the baculovirus and its host.

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Authors and Affiliations

  1. Department of Entomology, The Pennsylvania State University, 501 ASI Building, University Park, PA, 16802, USA

    Kelli Hoover & Michael J. Grove

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  1. Kelli Hoover
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  2. Michael J. Grove
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Correspondence to Kelli Hoover.

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Foundation item: Partial funding for this project was provided by the National Science Foundation USA (Award No. IBN-0077710).

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Hoover, K., Grove, M.J. Specificity of developmental resistance in gypsy moth (Lymantria dispar) to two DNA-insect viruses. Virol. Sin. 24, 493–500 (2009). https://doi.org/10.1007/s12250-009-3053-0

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  • DOI: https://doi.org/10.1007/s12250-009-3053-0

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  • R373

Key words

  • Resistance
  • Co-evolution
  • Baculovirus
  • Entomopoxvirus
  • Gypsy moth