Virologica Sinica

, 24:458 | Cite as

Genetic variation in field populations of baculoviruses: Mechanisms for generating variation and its potential role in baculovirus epizootiology

Article

Abstract

Baculoviridae is a family of insect-specific DNA viruses that have been used as biological control agents for insect pest control. In most cases these baculovirus control agents are natural field isolates that have been selected based on their infectivity and virulence. The advent of molecular tools such as restriction endonucleases, targeted polymerase chain reaction and new DNA sequencing strategies have allowed for efficient detection and characterization of genotypic variants within and among geographic and temporal isolates of baculovirus species. It has become evident that multiple genotypic variants occur even within individual infected larvae. Clonal strains of baculovirus species derived either by in vitro or in vivo approaches have been shown to vary with respect to infectivity and virulence. Many of the cell culture derived plague-purified strains have deletions that interrupt egt expression leading to virus strains that kill infected hosts more quickly. As well, in vitro clones often involve larger genomic deletions with the loss of pif gene function, resulting in strains deficient for oral infectivity. There are an increasing number of baculovirus species for which complete genome sequences are available for more than one strain or field isolate. Results of comparative analysis of these strains indicated that hr regions and bro genes often mark “hot spots” of genetic variability between strains and of potential recombination events. In addition, the degree of nucleotide polymorphisms between and within strains and their role in amino acid substitutions within ORFs and changes in promoter motifs is also beginning to be appreciated. In this short review the potential mechanisms that generate and maintain this genetic diversity within baculovirus populations is discussed, as is the potential role of genetic variation in host-pathogen interactions.

Key words

Baculoviridae Isolate Strain Genetic diversity Genome sequence 

CLC number

R373 

References

  1. 1.
    Adams J R, McClintock J T. 1991. Nuclear polyhedrosis viruses in insects. In: Atlas of Invertebrate Viruses (Adams J R, Bonami J R. ed.), Boca Raton: CRC Press, FL, USA. p89–204.Google Scholar
  2. 2.
    Blissard G W, Rohrmann G F. 1990. Baculovirus diversity and molecular biology. Ann Rev Entomol, 35: 127–155.CrossRefGoogle Scholar
  3. 3.
    Brown D A, Evans H F, Allen C J, et al. 1981. Biological and biochemical investigations of five European isolates of Mamestra brassicae nuclear polyhedrosis virus. Arch Virol, 69, 209–217.CrossRefPubMedGoogle Scholar
  4. 4.
    Bull J C, Godfray H C, O’Reilly D R. 2001. Persistence of an occlusion-negative recombinant nucleopolyhedrovirus in Trichoplusia ni indicates high multiplicity of cellular infection. Appl Environ Microbiol, 67: 5204–5209.CrossRefPubMedGoogle Scholar
  5. 5.
    Chen X, IJkel W F, Tarchini R, et al. 2001. The sequence of the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus genome. J Gen Virol, 82: 241–257.PubMedGoogle Scholar
  6. 6.
    Cooper D, Cory J S, Myers J H. 2003a. Hierarchical spatial structure of genetically variable nucleopolyhedroviruses infecting cyclic populations of western tent caterpillars. Mol Ecol, 12: 881–890.CrossRefPubMedGoogle Scholar
  7. 7.
    Cooper D, Cory J S, Theilmann D A, et al. 2003b. Nucleopolyhedroviruses of forest and western tent caterpillars: cross-infectivity and evidence for activation of latent virus in high-density field populations. Ecol Entomol, 28: 41–50.CrossRefGoogle Scholar
  8. 8.
    Cory J S, Hails R S, Sait S M. 1997. Baculovirus ecology. In: The Baculoviruses (Miller L K. ed.), New York: Plenum Press. p301–339.Google Scholar
  9. 9.
    Cory J S, Green B M, Paul R K, et al. 2005. Genotypic and phenotypic diversity of a baculovirus population within an individual insect host. J Invert Pathol, 89: 101–111.CrossRefGoogle Scholar
  10. 10.
    Crook N E. 1986. Restriction enzyme analysis of granulosis viruses isolated from Artogeia rapae and Pieris brassicae. J Gen Virol, 67: 781–787.CrossRefGoogle Scholar
  11. 11.
    Crook N E, Spencer R A, Payne C C, et al. 1985. Variation in Cydia pomonella granulosis virus isolates and physical maps of the DNA from three variants. J Gen Virol, 66: 2423–2430.CrossRefGoogle Scholar
  12. 12.
    Crozier G, Ribeiro H C T. 1992. Recombination as a possible major cause of genetic heterogeneity in Anticarsia gemmatalis nuclear polyhedrosis virus wild populations. Virus Res, 26: 183–196.CrossRefGoogle Scholar
  13. 13.
    Eberle K E, Sayed S, Rezapanah M, et al, 2009. Diversity and evolution of the Cydia pomonella granulovirus. J Gen Virol, 90: 662–671.CrossRefPubMedGoogle Scholar
  14. 14.
    Ebling P M, Kaupp W J. 1995. Differentiation and comparative activity of six isolates of nuclear polyhedrosis virus from the forest tent caterpillar, Malacosoma disstria, Hubner. J Invert Pathol, 66: 198–200.CrossRefGoogle Scholar
  15. 15.
    Erlandson M A. 1990. Biological and biochemical comparison of Mamestra configurata and Mamestra brassicae nuclear polyhedrosis virus isolates pathogenic for the bertha armyworm, Mamestra configurata (Lepidoptera: Noctuidae). J Invert Pathol, 56: 47–56.CrossRefGoogle Scholar
  16. 16.
    Erlandson M A, Baldwin D, Havereon M, et al. 2006. Isolation and characterization of plaque purified strains of Malacosoma disstria nucleopolyhedrovirus. Can J Microbiol, 52: 266–271.CrossRefPubMedGoogle Scholar
  17. 17.
    Fan Q, Li S, Wang L, et al. 2007. The genome sequence of the mulitnucleocapsid nucleopolyhedrovirus of the Chinese oak silkworm Antherea pernyi. Virology, 366: 304–315.CrossRefPubMedGoogle Scholar
  18. 18.
    Figueiredo E, Muñzo D, Murillo R, et al. 2009. Diversity of Iberian nucleopolyhedrovirus wild-type isolates infecting Helicoverpa armigera (Lepidoptera: Noctuidae). Biological Control, 50, 43–49.CrossRefGoogle Scholar
  19. 19.
    Goto C, Minobe Y, Iizuka T. 1992. Restriction analysis and mapping of the genomes of granulosis viruses isolated from Xestia c-nigrum and five other noctuid species. J GenVirol, 73: 1491–1497.Google Scholar
  20. 20.
    Graham R I, Tyne W I, Possee R D, et al. 2004. Genetically variable nucleopolyhedroviruses isolated from spatially separate populations of the winter moth Operophtera brumata (Lepidoptera: Geometridae) in Orkney. J Invert Pathol, 87: 29–38.CrossRefGoogle Scholar
  21. 21.
    Harrison R L, Puttler B, Popham H J R. 2008. Genomic sequence analysis of a fast-killing isolate of Spodoptera frugiperda multiple nucleopolyhedrovirus. J Gen Virol, 89: 775–790.CrossRefPubMedGoogle Scholar
  22. 22.
    Hitchman R B, Hodgson D J, King L A, et al. 2007. Host mediated selection of pathogen genotypes as a mechanism for the maintenance of baculovirus diversity in the field. J Invert Pathol, 94, 153–162.CrossRefGoogle Scholar
  23. 23.
    Hodgson D J, Vanbergen A J, Hartley S E, et al. 2002. Differential selection of baculovirus genotypes mediated by different species of host food plant. Ecol Lett, 5: 512–518.CrossRefGoogle Scholar
  24. 24.
    Hodgson D J, Vanbergen A J, Watt A D, et al. 2001. Phenotypic variation between naturally co-existing genotypes of a Lepidopteran baculovirus. Evol Ecol Res, 3: 687–701.Google Scholar
  25. 25.
    Jehle J A, Fritsch E, Huber J, et al. 2003. Intra-specific and inter-specific recombination of tortricid-specific granuloviruses during co-infection in insect larvae. Arch Virol, 148: 1317–1333.CrossRefPubMedGoogle Scholar
  26. 26.
    Jehle J A, Blissard G W, Bonning B C, et al. 2006. On theclassification and nomenclature of baculoviruses: a proposal for revision. Arch Virol, 151, 1257–1266.CrossRefPubMedGoogle Scholar
  27. 27.
    Jia F, Xu J, Nie Y C, et al. 2003. Comparison of Heliothis armigera NPV VHA273 wild isolate with its clone. Viroligica Sinica, 18: 241–245.Google Scholar
  28. 28.
    Kamiya K, Zhu J, Murata M, et al. 2004. Cloning and comparative characterization of three distinct nucleopolyhedroviruses isolated from the common cutworm, Spodoptera litura (Lepidoptera: Noctuidae) in Japan. Biol Contr, 31: 38–48.CrossRefGoogle Scholar
  29. 29.
    Keddie A B, Erlandson M A. 1995. Characterization of a nuclear polyhedrosis virus from the forest tent caterpillar, Malacosoma disstria. J Invert Pathol, 65: 43–47.CrossRefGoogle Scholar
  30. 30.
    Knell J D, Summers M D. 1981. Investigation of genetic heterogeneity in wild isolates of Spodoptera frugiperda nuclear polyhedrosis virus by restriction endonuclease analysis of plaque-purified mutants. Virology, 112: 190–197.CrossRefPubMedGoogle Scholar
  31. 31.
    Kolodny-Hirsch D M, Van Beek N A M. 1997. Selection of a morphological variant of Autographa californica nuclear polyhedrosis virus with increased virulence following serial passage in Plutella xylostella. J Invert Pathol, 69: 205–211.CrossRefGoogle Scholar
  32. 32.
    Lee H H, Miller L K. 1978. Isolation of genotypic variants of Autographa californica nuclear polyhedrosis virus. J Virol, 27: 754–767.PubMedGoogle Scholar
  33. 33.
    Li L, Donly C, Li Q, et al. 2002. Identification and genomic analysis of a second species of nucleopolyhedrovirus isolated from Mamestra configurata. Virology, 297: 226–244.CrossRefPubMedGoogle Scholar
  34. 34.
    Li L, Li Q, Willis L G, et al. 2005. Complete Comparative Genomic Analysis of Two Field Isolates of Mamestra configurata Nucleopolyhedrovirus-A. J Gen Virol, 86: 91–105CrossRefPubMedGoogle Scholar
  35. 35.
    Li Q, Donly C, Li L, et al. 2002. Sequence and organization of the Mamestra configurata nucleopolyhedrovirus genome. Virology, 294, 106–121.CrossRefPubMedGoogle Scholar
  36. 36.
    López Ferber M, Argaud O, Croizier L, et al. 2001. Diversity, distribution, and mobility of bro gene sequences in Bombyx mori nucleopolyhedrovirus. Virus Genes, 22: 247–254.CrossRefPubMedGoogle Scholar
  37. 37.
    Lynn D E, Shapiro M, Dougherty E M. 1993. Selection and screening of clonal isolates of the Abington strain of gypsy moth nuclear polyhedrosis virus. J Invert Pathol, 62: 191–195.CrossRefGoogle Scholar
  38. 38.
    Maeda S, Kamita S G, Kondo A. 1993. Host range expansion of Autographa califomica nuclear polyhedrosis virus (NPV) following recombination of a 0.6 kbp DNA fragment originating from Bombyx mori NPV. J Virol, 67: 6234–6238.PubMedGoogle Scholar
  39. 39.
    McIntosh A H, Rice W C, Ignoffo C M. 1987. Genotypic variants in wild-type populations of baculovirus. In Biotechnology in Invertebrate Pathology and Cell Culture (Maramorosch K. ed.), New York: Academic Press, p305–325.Google Scholar
  40. 40.
    Muñoz D, Castillejo J I, Caballero P. 1998. Naturally occurring deletion mutants are parasitic genotypes in a wild-type nucleopolyhedrovirus population of Spodoptera exigua. Appl Environ Microbiol, 64: 4372–4377.PubMedGoogle Scholar
  41. 41.
    Muñoz D, Murillo R, Krell P J, et al. 1999. Four genotypic variants of a Spodoptera exigua Nucleopolyhedrovirus (Se-SP2) are distinguished by a hypervariable genomic region. Virus Res, 59: 61–74.CrossRefPubMedGoogle Scholar
  42. 42.
    Muñoz D, Caballero P. 2000. Persistence and effects of parasitic genotypes in a mixed population of the Spodoptera exigua nucleopolyhedrovirus. Biol Contr, 19: 259–264.CrossRefGoogle Scholar
  43. 43.
    Nie Z M, Zhang Z F, Wang D, et al. 2007. Complete sequence and organization of Anteraea pernyi nucleopolyhedrovirus, a dr-rich baculovirus. BMC Genomics, 8: 248CrossRefPubMedGoogle Scholar
  44. 44.
    Ogembo J G, Chaeychomsri S, Kamiya K, et al. 2007. Cloning and comparative characterization of nucleopolyhedroviruses isolated from African bollworm, Helicoverpa armigera, (Lepidoptera: Noctudiae) in different geographic regions. J Insect Biotechnol Sericol, 76: 39–49.Google Scholar
  45. 45.
    Pijlman G P, van den Born E, Martens D E, et al. 2001. Autographa californica baculoviruses with large genomic deletions are rapidly generated in infected insect cells. Virology 283:132–138.CrossRefPubMedGoogle Scholar
  46. 46.
    Shapiro D I, Fuxa J R, Braymer HD, et al. 1991. DNA restriction polymorphism in wild isolates of Spodoptera frugiperda nuclear polyhedrosis virus. J Invert Pathol, 58: 96–105.CrossRefGoogle Scholar
  47. 47.
    Simón O, Williams T, López-Ferber M, et al. 2004. Genetic structure of a Spodoptera frugiperda nucleopolyhedrovirus population: High prevalence of deletion genotypes. Appl Environ Microbiol, 70: 5579–5588.CrossRefPubMedGoogle Scholar
  48. 48.
    Simón O, Williams T, López-Ferber M, et al. 2005. Functional importance of deletion mutant genotypes in an insect nucleopolyhedrovirus population. Appl Environ Microbiol, 71: 4254–4262.CrossRefPubMedGoogle Scholar
  49. 49.
    Smith I R L, Crook N E. 1988. In vivo isolation of baculovirus genotypes. Virology, 166: 240–244.CrossRefPubMedGoogle Scholar
  50. 50.
    Smith G E, Summers M D. 1979. Restriction Maps of Five Autographa californica MNPV Variants, Trichoplusia ni MNPVand Galleria mellonella MNPV DNAs with restriction endonucleases SmaI, KpnI, BamHI, SacI, XhoI, and EcoRI. J Virol, 30: 828–838.PubMedGoogle Scholar
  51. 51.
    Stiles B, Himmerich S. 1998. Autographa californica NPV isolates: Restriction endonuclease analysis and comparative biological activity. J Invert Pathol, 72, 174–177.CrossRefGoogle Scholar
  52. 52.
    Theilmann D A, Blissard G W, Bonning B, et al. 2005. Family Baculoviridae. In: Virus Taxonomy, Eighth Report of the International Committee on Virus Taxonomy (Fauquet C M, Mayo M A, Maniloff J, et al. ed.), San Diego: Elsevier Press, p177–185.Google Scholar
  53. 53.
    van Oers M M, Vlak J M. 2007. Baculovirus Genomics. Current Drug Targets, 8: 1051–1068.CrossRefPubMedGoogle Scholar
  54. 54.
    Vlak J M, Gröner A. 1980. Identification of two nuclear polyhedrosis viruses from the cabbage moth, Mamestra brassicae (Lepidoptera: Noctuidae). J Invert Pathol, 35, 269–278.CrossRefGoogle Scholar
  55. 55.
    Vickers J M, Cory J S, Entwistle P F. 1991. DNA characterization of eight geographic isolates of granulosis virus from the potato tuber moth (Phthorimaea operculella) (Lepidoptera, Gelechiidae). J Invert Pathol, 57, 334–342.CrossRefGoogle Scholar
  56. 56.
    Wolff J L C, Vaicente F H, Martins R, et al. 2008. Analysis of the genome of Spodoptera frugiperda nucleopolyhedrovirus (SfMNPV-19) and of the high genomic heterogeneity in Group II nucleopolyhedroviruses. J Gen Virol, 89, 1202–1211.CrossRefPubMedGoogle Scholar
  57. 57.
    Yanase Y, Hashimoto Y, Kawarabata T. 2000. Identification of insertion and deletion genes in Autographa californica nucleopolyhedrovirus variants isolated from Galleria mellonella, Spodoptera exigua, Spodoptera litura and Xestia c-nigrum. Virus Genes, 21: 167–177.CrossRefPubMedGoogle Scholar
  58. 58.
    Zhang C X, Ma X C, Guo Z J. 2005. Comparison of the complete genome sequence between C1 and G4 isolates of the Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus. Virology, 333(1):190–199.CrossRefPubMedGoogle Scholar

Copyright information

© Wuhan Institute of Virology, CAS and Springer Berlin Heidelberg 2009

Authors and Affiliations

  1. 1.Agriculture and Agri-Food CanadaSaskatoon Research CentreSaskatoonCanada

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