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HCMV infection depress NGF expression in human glioma cells


Human cytomegalovirus (HCMV) is the most common cause of congenital infection, resulting in birth defects such as microcephaly. In this study, RT-PCR and Western Blotting were performed to quantify the regulation of endogenic nerve growth factor expression in neuroglia cells by HCMV infection. The results showed that basal, endogenous NGF expression in U251 was unchanged during early HCMV infection. NGF expression is strongly down-regulated during the latent phase of infection. These results suggest that HCMV can depress the NGF expression in U251 cells.

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  1. Arvidsson A, Collin T, Kirik D, et al. 2002. Neuronal replacement from endogenous precursors in the adult brain after stroke. Nat Med, 8(9): 963–970.

    Article  PubMed  CAS  Google Scholar 

  2. Benoit B O, Savarese T, Joly M, et al. 2001. Neurotrophin channeling of neural progenitor cell differentiation. J Neurobiol, 46(4): 265–280.

    Article  PubMed  CAS  Google Scholar 

  3. Cattaneo E, McKay R. 1990. Proliferation and differentiation of neuronal stem cells regulated by nerve growth factor. Nature, 347(6295): 762–765.

    Article  PubMed  CAS  Google Scholar 

  4. D’Ambrosi N, Murra B, Vacca F, et al. 2004. Pathways of survival induced by NGF and extracellular ATP after growth factor deprivation. Prog Brain Res, 146:93–100.

    Article  PubMed  Google Scholar 

  5. Darsalia V, Kallur T, Kokaia Z. 2007. Survival, migration and neuronal differentiation of human fetal striatal and cortical neural stem cells grafted in stroke-damaged rat striatum. Eur J Neurosci, 26(3): 605–614.

    Article  PubMed  Google Scholar 

  6. Deckwerth T L, Johnson E M Jr. 1993. Neurotrophic factor deprivation-induced death. Ann N Y Acad Sci, 679: 121–131.

    Article  PubMed  CAS  Google Scholar 

  7. Furukawa S, Furukawa Y. 1990. Nerve growth factor synthesis and its regulatory mechanisms: an approach to therapeutic induction of nerve growth factor synthesis. Cerebrovasc Brain Metab Rev, 2(4): 328–344.

    PubMed  CAS  Google Scholar 

  8. Furukawa S, Furukawa Y, Satoyoshi E, et al. 1987. Synthesis/secretion of nerve growth factor is associated with cell growth in cultured mouse astroglial cells. Biochem Biophys Res Commun, 142(2): 395–402.

    Article  PubMed  CAS  Google Scholar 

  9. Guzman R, Bliss T, De Los Angeles A, et al. 2008. Neural progenitor cells transplanted into the uninjured brain undergo targeted migration after stroke onset. J Neurosci Res, 86(4): 873–882.

    Article  PubMed  CAS  Google Scholar 

  10. Hagay Z J, Biran G, Ornoy A, et al. 1996. Congenital cytomegalovirus infection: a long-standing problem still seeking a solution. Am J Obstet Gynecol, 174(1 Pt 1): 241–245.

    Article  PubMed  CAS  Google Scholar 

  11. Ignatovich V F. 1975. Enhancement of the antigenic activity and virulence of the vaccine strain E of Rickettsia prow azeki by passages in cell culture. Acta Virol, 19(6): 481–485.

    PubMed  CAS  Google Scholar 

  12. Levi-Montalcini R. 1987. The nerve growth factor 35 years later. Science, 237(4819): 1154–1162.

    Article  PubMed  CAS  Google Scholar 

  13. Lokensgard J R, Cheeran M C, Gekker G, et al. 1999. Human cytomegalovirus replication and modulation of apoptosis in astrocytes. J Hum Virol, 2(2): 91–101.

    PubMed  CAS  Google Scholar 

  14. Lu B, Yokoyama M, Dreyfus C F, et al. 1991. NGF gene expression in actively growing brain glia. J Neurosci, 11(2): 318–326.

    PubMed  CAS  Google Scholar 

  15. Mesner P W, Epting C L, Hegarty J L, et al. 1995. A timetable of events during programmed cell death induced by trophic factor withdrawal from neuronal PC12 cells. J Neurosci, 15(11): 7357–7366.

    PubMed  CAS  Google Scholar 

  16. Revello M G, Zavattoni M, Furione M, et al. 2006. Preconceptional primary human cytomegalovirus infection and risk of congenital infection. J Infect Dis, 193(6): 783–787.

    Article  PubMed  Google Scholar 

  17. Rich K M. 1992. Neuronal death after trophic factor deprivation. J Neurotrauma, 9Suppl 1:S61–69.

    PubMed  Google Scholar 

  18. Shinmura Y, Kosugi I, Kaneta M, et al. 1999. Migration of virus-infected neuronal cells in cerebral slice cultures of developing mouse brains after in vitro infection with murine cytomegalovirus. Acta Neuropathol, 98(6): 590–596.

    Article  PubMed  CAS  Google Scholar 

  19. Sofroniew M V, Howe C L, Mobley W C. 2001. Nerve growth factor signaling, neuroprotection, and neural repair. Annu Rev Neurosci, 24: 1217–1281.

    Article  PubMed  CAS  Google Scholar 

  20. Trincado D E, Rawlinson W D. 2001. Congenital and perinatal infections with cytomegalovirus. J Paediatr Child Health, 37(2): 187–192.

    Article  PubMed  CAS  Google Scholar 

  21. Ziegler C G, Sicard F, Sperber S, et al. 2006. DHEA reduces NGF-mediated cell survival in serum-deprived PC12 cells. Ann N Y Acad Sci, 1073: 306–311.

    Article  PubMed  CAS  Google Scholar 

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Correspondence to Bin Wang.

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Foundation items: National Natural Science Foundation of China (30770105); Mt. Tai Scholar Construction Engineering Special Foundation of Shandong province.

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Wang, Ht., Wang, B., Liu, Zj. et al. HCMV infection depress NGF expression in human glioma cells. Virol. Sin. 24, 209–214 (2009).

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  • R373

Key words

  • Human cytomegalovirus (HCMV)
  • U251 cell
  • Nerve growth factor (NGF)