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HCMV infection depress NGF expression in human glioma cells

Virologica Sinica volume 24, pages 209–214 (2009)Cite this article

Abstract

Human cytomegalovirus (HCMV) is the most common cause of congenital infection, resulting in birth defects such as microcephaly. In this study, RT-PCR and Western Blotting were performed to quantify the regulation of endogenic nerve growth factor expression in neuroglia cells by HCMV infection. The results showed that basal, endogenous NGF expression in U251 was unchanged during early HCMV infection. NGF expression is strongly down-regulated during the latent phase of infection. These results suggest that HCMV can depress the NGF expression in U251 cells.

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Authors and Affiliations

  1. Department of Microbiology, Qingdao Medical College, and Key Laboratory of Medicine and Biotechnology of Qingdao, Qingdao University Medical College, Qingdao, 266071, China

    Hai-tao Wang, Bin Wang, Zhi-qiang Bai, Ling Li, Dong-meng Qian, Zhi-yong Yan & Xu-xia Song

  2. Qingdao Center for Disease control and Prevention, Qingdao, 266033, China

    Hai-tao Wang

  3. LRB 270E, University of Massachusetts Medical School, Worcester, MA, 01605, USA

    Zhi-jun Liu

Authors
  1. Hai-tao Wang
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  2. Bin Wang
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  3. Zhi-jun Liu
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  4. Zhi-qiang Bai
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  5. Ling Li
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  6. Dong-meng Qian
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  7. Zhi-yong Yan
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  8. Xu-xia Song
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Corresponding author

Correspondence to Bin Wang.

Additional information

Foundation items: National Natural Science Foundation of China (30770105); Mt. Tai Scholar Construction Engineering Special Foundation of Shandong province.

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Wang, Ht., Wang, B., Liu, Zj. et al. HCMV infection depress NGF expression in human glioma cells. Virol. Sin. 24, 209–214 (2009). https://doi.org/10.1007/s12250-009-3018-3

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  • DOI: https://doi.org/10.1007/s12250-009-3018-3

CLC number

  • R373

Key words

  • Human cytomegalovirus (HCMV)
  • U251 cell
  • Nerve growth factor (NGF)