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Application of VP1 protein to develop monoclonal antibody against foot-and-mouth disease virus Asia1 type

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In order to develop an anti-FMDV Asia1 type monoclonal antibody (mAb), BABL/c mice were immunized with recombinant FMDV VP1 protein. Three mAbs, 1B8, 5E1 and 5E2, were then further optimized. The result indicated that prepared anti-FMDV Asia1 mAbs had no cross-reactivity with Swine vesicular disease (SVD) and FMDV O, A and C type antigen. Their titers in abdomen liquor were 1:5×106, 1:2×106 and 1:5×106, respectively. 1B8 was found to be of IgG1 subtype, 5E1 and 5E2 belonged to IgG2b subtype. In this study, the prepared mAbs are specific for detecting FMDV type Asia1, and is potentially useful for pen-side diagnosis.

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  1. Barteling S J. 2004. Modern inactivated foot-and mouth disease vaccines: historical background and key elements in production and use. In: Foot-and-Mouth Disease: Current Perspectives (Sobrino F, Domingo E. ed.). England: Horizon Bioscience, p305–333.

    Google Scholar 

  2. Du J Z, Chang H Y, Cong G Z, et al. 2005. Molecular characteristics and expression of structural protein gene of Foot-and-Mouth Disease virus type Asia1. Virol Sin, 20(1): 65–69.

    CAS  Google Scholar 

  3. Davis J M, Pennington J E, Kubler A M, et al. 1982. A simple, single-step technique for selecting and cloning hybridomas for the production of monoclonal antibodies. J Immunol Methods, 50: 161–171.

    Article  PubMed  CAS  Google Scholar 

  4. Freiberg B, Hohlich B, Haas B, et al. Type-independent detection of foot-and-mouth disease virus by monoclonal antibodies that bind to amino-terminal residues of capsid protein VP2. J Virol Methods, 92: 199–205.

  5. Golding S M, Hedger R S. 1976. Radial immunodiffusion and serum neutralization techniques for the assay of antibodies to swine vesicular disease. Res Vet Sci, 20:142–147.

    PubMed  CAS  Google Scholar 

  6. Guo H, Liu Z, Sun S, et al. 2005. Immune response in guinea pigs vaccinated with DNA vaccine of foot-andmouth disease virus O/China99. Vaccine, 23: 3236–3242.

    Article  PubMed  CAS  Google Scholar 

  7. Jackson T, King A M, Stuart D I, et al. 2003. Structure and receptor binding. Virus Res, 91: 33–46.

    Article  PubMed  CAS  Google Scholar 

  8. Klein J, Hussain M, Ahmad M, et al. 2007. Genetic characterisation of the recent foot-and-mouth disease virus subtype A/IRN/2005. Virol J, 4: 122.

    Article  PubMed  Google Scholar 

  9. Kovacs-Nolan J, Sasaki E, Yoo D, et al. 2001. Cloning and expression of human rotavirus spike protein, VP8*, in Escherichia coli. Biochem Biophys Res Commun, 282:1183–1188.

    Article  PubMed  CAS  Google Scholar 

  10. Laemmli U K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. 227(5259): 680–685.

    CAS  Google Scholar 

  11. Lundberg M, Holmgren A, Johansson M. 2006. Human glutaredoxin 2 affinity tag for recombinant peptide and protein purification. Protein Expr Purif, 45: 37–42.

    Article  PubMed  CAS  Google Scholar 

  12. Smith D B. 2000. Generating fusions to glutathione S-transferase for protein studies. Methods Enzymol, 326:254–270.

    Article  PubMed  CAS  Google Scholar 

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Correspondence to Hui-yun Chang.

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Foudation items: The National high Technology Research and Development Program of China (No.2006AA10A204); The National science & Technology Pillar Program (No. 2006BAD06A17)

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Lin, T., Du, Jz., Shao, Jj. et al. Application of VP1 protein to develop monoclonal antibody against foot-and-mouth disease virus Asia1 type. Virol. Sin. 24, 215–220 (2009).

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  • R373

Key words

  • Asia1 type FMDV
  • VP1 protein
  • Monoclonal antibody