Virologica Sinica

, Volume 23, Issue 5, pp 345–351 | Cite as

An improved culture system for virus isolation and detection

  • Yu-chen Xia
  • Zhi-hong Hu
  • Zhi-juan Qiu
  • Zhong-bin Ma
  • Hua-lin Wang
  • Fei Deng
Article
  • 69 Downloads

Abstract

Cell culture plays an important role in virology. It provides a platform for the detection and isolation of viruses as well as for the biochemistry and molecular biology based studies of viruses. In the present work, a new system that could permits multiple (different) cell lines to be simultaneously cultured in one dish was developed. In the system, each cell line was cultured in an isolated zone in the same dish or well and the system is therefore called an isolated co-culture system. The usefulness of this novel approach for virus isolation was demonstrated using a model system based on adenovirus.

Key words

Cell culture Isolated co-culture Viral amplification Diagnosis 

CLC number

Q939.4 

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References

  1. 1.
    Allard A, Girones R, Juto P, et al. 1990. Polymerase chain reaction for detection of adenoviruses in stool samples. J Clin Microbiol, 28: 2659–2667.PubMedGoogle Scholar
  2. 2.
    Brewer L A, Lwamba H C, Murtaugh M P, et al. 2001. Porcine encephalomyocarditis virus persists in pig myocardium and infects human myocardial cells. J Virol, 75: 11621–11629.PubMedCrossRefGoogle Scholar
  3. 3.
    Brumback B G, Wade C D. 1994. Simultaneous culture for adenovirus, cytomegalovirus, and herpes simplex virus in same shell vial by using three-color fluorescence. J Clin Microbiol, 32: 2289–2290.PubMedGoogle Scholar
  4. 4.
    Diane S L, Christine C G. 2007. Role of Cell Culture for Virus Detection in the Age of Technology. Clin Microbiol Rev, 20: 49–78.CrossRefGoogle Scholar
  5. 5.
    Fedorko D P, Nelson N A, McAuliffe J M, et al. 2006. Performance of the rapid tests for detection of avian influenza A virus types H5N1 and H9N2. J Clin Microbiol, 44: 1596–1597.PubMedCrossRefGoogle Scholar
  6. 6.
    Gillim R L, Taylor J, Scholl D R, et al. 2004. Discovery of novel human and animal cells infectedby the severe acute respiratory syndrome coronavirus by replication specific multiplex reverse transcription-PCR. J Clin Microbiol, 42: 3196–3206.CrossRefGoogle Scholar
  7. 7.
    Harit A K, Ichhpujani R L, Gupta S, et al. 2006. Nipah/Hendra virus outbreak in Siliguri, West Bengal, India in 2001. Indian J Med Res, 123: 553–560.PubMedGoogle Scholar
  8. 8.
    Harrison R. 1907. Observations on the living developing nerve fiber. Anat Rec, 1: 116–128.CrossRefGoogle Scholar
  9. 9.
    Hsiung G D. 1984. Diagnostic virology: from animals to automation. Yale J Biol Med, 57: 727–733.PubMedGoogle Scholar
  10. 10.
    Kaye M, Druce J, Tran T, et al. 2006. SARS-associated coronavirus replication in cell lines. Emerg Infect Dis, 12: 128–133.PubMedGoogle Scholar
  11. 11.
    Ksiazek T G, Erdman D, Goldsmith C S, et al. 2003. A novel coronavirus associated with severe acute respiratory syndrome. N Engl J Med, 348: 1953–1966.PubMedCrossRefGoogle Scholar
  12. 12.
    Li L, Wo J, Shao J, et al. 2003. SARS-coronavirus replicates in mononuclear cells of peripheral blood (PBMCs) from SARS patients. J Clin Virol, 28: 239–244.PubMedCrossRefGoogle Scholar
  13. 13.
    Mossel E C, Huang C, Narayanan K, et al. 2005. Exogenous ACE2 expression allows refractory cell lines to support severe acute respiratory syndrome coronavirus replication. J Virol, 79: 3846–3850.PubMedCrossRefGoogle Scholar
  14. 14.
    Peiris J S, Chu C M, Cheng V C, et al. 2003. Coronavirus as a possible cause of severe acute respiratory syndrome. Lancet, 361: 1319–1325.PubMedCrossRefGoogle Scholar
  15. 15.
    Ran A M, Hartwig N G, Bestebroer T M, et al. 2004. A previously undescribed coronavirus associated with respiratory disease in humans. Proc. Natl. Acad. Sci, 101: 6212–6216.CrossRefGoogle Scholar
  16. 16.
    Ray C G, Minnich L L. 1987. Efficiency of immunofluorescence for rapid detection of common respiratory viruses. J Clin Microbiol, 25: 355–357.PubMedGoogle Scholar
  17. 17.
    Schildgen O, Jebbink M F, Vries M, et al. 2006. Identification of cell lines permissive for human coronavirus NL63. J Virol Methods, 138: 207–210.PubMedCrossRefGoogle Scholar
  18. 18.
    Wen H L, Moore M J, Vasilieva N, et al. 2003. Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature, 426: 450–454.CrossRefGoogle Scholar
  19. 19.
    Yuen K Y, Chang P K, Peiris J S, et al. 1998. Clinical features and rapidviral diagnosis of human disease associated with avian influenza A H5N1 virus. Lancet, 351: 467–471.PubMedCrossRefGoogle Scholar

Copyright information

© Wuhan Institute of Virology, CAS and Springer-Verlag GmbH 2008

Authors and Affiliations

  • Yu-chen Xia
    • 1
  • Zhi-hong Hu
    • 1
  • Zhi-juan Qiu
    • 1
  • Zhong-bin Ma
    • 1
  • Hua-lin Wang
    • 1
  • Fei Deng
    • 1
  1. 1.State Key Laboratory of Virology, Wuhan Institute of VirologyChinese Academy of SciencesWuhanChina

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