Virologica Sinica

, Volume 23, Issue 5, pp 345–351 | Cite as

An improved culture system for virus isolation and detection

  • Yu-chen Xia
  • Zhi-hong Hu
  • Zhi-juan Qiu
  • Zhong-bin Ma
  • Hua-lin Wang
  • Fei Deng


Cell culture plays an important role in virology. It provides a platform for the detection and isolation of viruses as well as for the biochemistry and molecular biology based studies of viruses. In the present work, a new system that could permits multiple (different) cell lines to be simultaneously cultured in one dish was developed. In the system, each cell line was cultured in an isolated zone in the same dish or well and the system is therefore called an isolated co-culture system. The usefulness of this novel approach for virus isolation was demonstrated using a model system based on adenovirus.

Key words

Cell culture Isolated co-culture Viral amplification Diagnosis 

CLC number



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Copyright information

© Wuhan Institute of Virology, CAS and Springer-Verlag GmbH 2008

Authors and Affiliations

  • Yu-chen Xia
    • 1
  • Zhi-hong Hu
    • 1
  • Zhi-juan Qiu
    • 1
  • Zhong-bin Ma
    • 1
  • Hua-lin Wang
    • 1
  • Fei Deng
    • 1
  1. 1.State Key Laboratory of Virology, Wuhan Institute of VirologyChinese Academy of SciencesWuhanChina

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