Virologica Sinica

, Volume 23, Issue 4, pp 233–246 | Cite as

Comparison of the immunogenicities of HIV-1 mutants based on structural modification of env

  • Jian-hui Nie
  • Chun-tao Zhang
  • Hui-hui Chong
  • Xue-ling Wu
  • Chun-yu Liu
  • Yu Wu
  • Chen-yan Zhao
  • Lin-qi Zhang
  • You-chun WangEmail author


Eleven env mutants were designed and generated by site-directed mutagenesis of the regions around NAb epitopes and deletions of variable regions in env. The immunogenicities of the generated mutants were evaluated using single-cycle infection neutralization assays with two pseudoviruses and IFN-γ ELISPOT. Overall, five mutants (dWt, M2, M5-2, M5-1 and dM7) induced higher neutralization activities for both pseudoviruses than plasmid Wt, while only two of the mutants (dWt and M5-2) showed significant differences (P<0.05). Two mutants (M2 and dM2) induced more Env-specific T cells than plasmid Wt. Statistically however, significance was only reached for mutant M2. Thus, properly modified HIV-1 Env may have the potential to induce potent cellular and humoral immune responses.

Key words

HIV-1 Env Modification Neutralization assay ELISPOT 

CLC number



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Copyright information

© Wuhan Institute of Virology, CAS and Springer-Verlag GmbH 2008

Authors and Affiliations

  • Jian-hui Nie
    • 1
  • Chun-tao Zhang
    • 1
  • Hui-hui Chong
    • 1
    • 2
  • Xue-ling Wu
    • 1
  • Chun-yu Liu
    • 1
  • Yu Wu
    • 1
  • Chen-yan Zhao
    • 1
  • Lin-qi Zhang
    • 3
  • You-chun Wang
    • 1
    Email author
  1. 1.Department of Cell BiologyNational Institute for the Control of Pharmaceutical and Biological ProductsBeijingChina
  2. 2.College of Life ScienceJilin UniversityChangchunChina
  3. 3.Aaron Diamond AIDS Research CenterThe Rockefeller UniversityNew YorkUSA

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