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One new method of nucleic acid amplification—Loop-mediated isothermal amplification of DNA

Virologica Sinica volume 23, pages 167–172 (2008)Cite this article

Abstract

Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method, which amplifies DNA with high specificity, sensitivity, rapidity and efficiency under isothermal conditions using a set of four specially designed primers and a Bst DNA polymerase with strand displacement activity. The basic principle, characteristics, development of LAMP and its applications are summarized in this article.

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References

  1. Aoi Y, Hosogai M, Tsuneda S, 2006. Real-time quantitative LAMP (loop-mediated isothermal amplification of DNA) as a simple method for monitoring ammonia-oxidizing bacteria. J Biotechnol, 125: 484–491.

    PubMed  Article  CAS  Google Scholar 

  2. Compton J. 1991. Nucleic acid sequence based amplification. Nature, 350: 91–92.

    PubMed  Article  CAS  Google Scholar 

  3. Dukes J P, King D P, Alexandersen S, et al. 2006. Novel reverse transcription loop-mediated isothermal amplification for rapid detection of foot-and-mouth disease virus. Arch Virol, 151: 1093–1106.

    PubMed  Article  CAS  Google Scholar 

  4. Fukuta S, Kato S, Yoshida K, 2003. Detection of tomato yellow leaf curl virus by loop-mediated isothermal amplification reaction. J Virol Methods, 112: 35–40.

    PubMed  Article  CAS  Google Scholar 

  5. Fukuta S, Ohishi K, Yoshida K, et al. 2004. Development of immunocapture reverse transcription loop-mediated isothermal amplification for the detection of tomato spotted wilt virus from chrysanthemum. J Virol Methods, 121: 49–55.

    PubMed  Article  CAS  Google Scholar 

  6. Guatelli J C, Whitfield K M, Kwoh D Y, et al. 1990. Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication. Proc Nat Acad Sci USA, 87: 1874–1878.

    PubMed  Article  CAS  Google Scholar 

  7. Hirayama H, Kageyama S, Takahashi Y, 2006. Rapid sexing of water buffalo (Bubalus bubalis) embryos using loop-mediated isothermal amplification. Theriogenology, 66: 1249–1256.

    PubMed  Article  CAS  Google Scholar 

  8. Hayashia N, Araib R, Tada S, 2007. Detection and identification of Brettanomyces/Dekkera sp. yeasts with a loop-mediated isothermal amplifcation method. Food Microbiol, 24, 778–785.

    Article  Google Scholar 

  9. Iwata S, Shibata Y, Kawada J, et al. 2006. Rapid detection of Epstein-Barr virus DNA by loop-mediated isothermal amplification method. J Clin Virol, 37: 128–133.

    PubMed  Article  CAS  Google Scholar 

  10. Imai M, Ninomiya A, Minekawa H, et al. 2007. Rapid diagnosis of H5N1 avian influenza virus infection by newly developed influenza H5 hemagglutinin gene-specific loop-mediated isothermal amplification method. J Virol Methods, 141: 173–180.

    PubMed  Article  CAS  Google Scholar 

  11. Kaneko H, Kawana T, Fukushima E, 2007. Tolerance of loop-mediated isothermal amplification to a culture medium and biological substances. J Biochem Biophys Methods, 70: 499–501.

    PubMed  Article  CAS  Google Scholar 

  12. Kuhara T, Yoshikawa T, 2007. Rapid detection of human herpesvirus 8 DNA using loop-mediated isothermal amplification. J Virol Methods, 144: 79–85.

    PubMed  Article  CAS  Google Scholar 

  13. Kurosaki Y, Takada A, Ebihara H, et al. 2007. Rapid and simple detection of Ebola virus by reverse transcription-loop-mediated isothermal amplification. J Virol Methods, 141: 78–83.

    PubMed  Article  CAS  Google Scholar 

  14. Lizardi P M, Huang X, Zhu Z, et al. 1998. Mutation detection and single molecule counting using isothermal rolling circle amplification. Nat Genet, 19: 225–232.

    PubMed  Article  CAS  Google Scholar 

  15. Mori Y, Nagamine K, Tomita N, et al. 2001. Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun, 289: 150–154.

    PubMed  Article  CAS  Google Scholar 

  16. Marlowe C A, Haraguchi I, et al. 2004. Rapid detection of a fish iridovirus using loop-mediated isothermal amplification (LAMP). J Virol Methods, 121: 155–161.

    Article  Google Scholar 

  17. Notomi T, Okayama H, Masubuchi H, et al. 2000. Loop-mediated isothermal amplification of DNA. Nucl Acid Res, 28: e63.

    Article  CAS  Google Scholar 

  18. Nagamine K, Hase T, Notomi T, 2002. Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol Cell Probes, 16: 223–229.

    PubMed  Article  CAS  Google Scholar 

  19. Saiki R K, Scharff S, Faloona F, et al. 1985. Enzymatic amplification of β-globin genomic sequences and restriction site analysis diagnosis of sickle cell anemia. Science, 230: 1350–1354.

    PubMed  Article  CAS  Google Scholar 

  20. Soliman H, El-Matbouli M, 2006. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) for rapid detection of viral hemorrhagic septicaemia virus (VHSV). Veterin Microbiol, 114: 205–213.

    Article  CAS  Google Scholar 

  21. Suzuki R, Yoshikawa T, Ihira M, 2006. Development of the loop-mediated isothermal amplification method for rapid detection of cytomegalovirus DNA. J Virol Methods, 132: 216–221.

    PubMed  Article  CAS  Google Scholar 

  22. Walker G T, Fraiser M S, Schram J L, et al. 1992. Strand displacement amplification-an isothermal, in vitro DNA amplification technique. Nucl Acid Res, 20: 1691–1696.

    Article  CAS  Google Scholar 

  23. Zhao F S, Chao Q H, Chun H R, 2006. Sensitive and rapid detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimps by loop-mediated isothermal amplification. J Virol Methods, 131: 41–46.

    Article  Google Scholar 

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Authors and Affiliations

  1. School of Life Science, Shanghai University, Shanghai, 200444, China

    Xue-en Fang & Qin Chen

  2. Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai, 200135, China

    Jian Li

Authors
  1. Xue-en Fang
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  2. Jian Li
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  3. Qin Chen
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Corresponding author

Correspondence to Qin Chen.

Additional information

Foundation items: Grant support from General Administration of Quality Supervision, Inspection and Quarantine of China (HK001-2007).

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Fang, Xe., Li, J. & Chen, Q. One new method of nucleic acid amplification—Loop-mediated isothermal amplification of DNA. Virol. Sin. 23, 167–172 (2008). https://doi.org/10.1007/s12250-008-2929-8

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  • DOI: https://doi.org/10.1007/s12250-008-2929-8

Key words

  • Nucleic acid amplification
  • Loop-mediated isothermal amplification (LAMP)
  • Application

CLC number

  • Q7