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One new method of nucleic acid amplification—Loop-mediated isothermal amplification of DNA

Abstract

Loop-mediated isothermal amplification (LAMP) is a novel nucleic acid amplification method, which amplifies DNA with high specificity, sensitivity, rapidity and efficiency under isothermal conditions using a set of four specially designed primers and a Bst DNA polymerase with strand displacement activity. The basic principle, characteristics, development of LAMP and its applications are summarized in this article.

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Correspondence to Qin Chen.

Additional information

Foundation items: Grant support from General Administration of Quality Supervision, Inspection and Quarantine of China (HK001-2007).

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Fang, Xe., Li, J. & Chen, Q. One new method of nucleic acid amplification—Loop-mediated isothermal amplification of DNA. Virol. Sin. 23, 167–172 (2008). https://doi.org/10.1007/s12250-008-2929-8

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  • DOI: https://doi.org/10.1007/s12250-008-2929-8

Key words

  • Nucleic acid amplification
  • Loop-mediated isothermal amplification (LAMP)
  • Application

CLC number

  • Q7