Virologica Sinica

, Volume 23, Issue 1, pp 73–77 | Cite as

Expression and assembly mechanism of the capsid proteins of a satellite virus (XSV) associated with Macrobrachium rosenbergii nodavirus

  • Jian-min Wang
  • Hua-jun Zhang
  • Zheng-li ShiEmail author


The extra small virus (XSV) is a satellite virus associated with Macrobrachium rosenbergii nodavirus (MrNV) and its genome consists of two overlapping ORFs, CP17 and CP16. Here we demonstrate that CP16 is expressed from the second AUG of the CP17 gene and is not a proteinase cleavage result of CP17. We further expressed CP17 and several truncated CP17s (in which the N-or C-terminus or both was deleted), respectively, in Escherichia coli. Except for the recombinant plasmid CP17δC10, all recombinant plasmids expressed soluble protein which assembled into virus-like particles (VLPs), suggesting that the C-terminus is important for VLP formation.

Key words

Extra small virus (XSV) Satellite virus Virus-like particle White tail disease 

CLC number



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Copyright information

© Wuhan Institute of Virology, CAS 2008

Authors and Affiliations

  1. 1.State Key Laboratory of Virology, Wuhan Institute of VirologyChinese Academy of Sciences (CAS)WuhanChina
  2. 2.Graduate School of CASBeijingChina

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