Pathogenicity of the Diatraea saccharalis densovirus (DsDNV) was tested on its host larvae. The results showed that up to 4 days after inoculation, no larvae mortality was observed and the infected larvae started to exhibit the infection symptoms from the fourth day. After 5 days of infection, the cumulative mortality of infected larvae increased significantly and reached 60% after 12 days and 100% after 21 days of infection, whereas that of the control group was only 10% and 20%, respectively, after same periods of infection, suggesting that the high mortality of infected larvae groups was due to the high pathogenicity of DsDNV. The size of the DsDNA was determined by Electron microscopy visualization of viral DNA molecules and gel electrophoresis of both native and endonuclease digested DNA fragments. The total length of the native DsDNA was about 5.95 kb. The DsDNV DNA was digested with 16 restriction enzymes and a restriction map of those enzymes was constructed with 41 restriction sites. Comparison of the restriction map of the DsDNV genome with those of the genomes of Junonia coenia densovirus (JcDNV) and Galleria mellonella densovirus (GmDNV) indicated that the three densovirus genomes were found to share many identical restriction sites. Thus, most of the restriction sites of the following endonucleases Bam H I, Hha I, Xba I, Cla I, Asp 700, Spe I, Nco I and Bcl I, were found to be conserved among the three densovirus genomes. Symmetrical cleavage sites mapped at the both ends of the genome suggested the presence of inverted terminal repeats (ITRs) whose size was estimated to be about 500 bp. The similar genome size, almost identical restriction sites and presence of an ITR of about 500 bp for these three densoviruses suggested that they belong to the same group of ambisense densoviruses.
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Afanasiev B N, Galyov E E, Buchatsky L P, et al. 1991. Nucleotide sequence and genomic organization of Aedes densonucleosis virus[J]. Virology, 185(1): 323–336.
Astele C R. 1990. Terminal hairpins of parvovirus genome and their role in DNA replication[M]. Tijssen P ed. Handbook of Parvoviruse. vol. 1. Boca Raton: CRC Press, 59–79.
Bergoin M, Tijssen P. 2000. Molecular biology of Densovirinae[M]. Faisst S, Rommelaere J, ed, Parvoviruses. From molecular biology to pathology and therapeutic uses. Karger: Basel, 12–32. 4.
Boublik Y, Jousset F X, Bergoin M. 1994. Complete nucleotide sequence and genomic organization of the Aedes albopictus parvovirus (AaPV) pathogenic for Aedes aegypti larvae [J]. Virology, 200: 752–763.
Carlson J, Suchman E, Buchatsky L. 2006. Densoviruses for control and genetic manipulation of mosquitoes[J]. Adv Virus Res, 68: 361–392.
Chao Y C, Young S Y, Kim K S. 1985. A newly isolated densonucleosis virus from Pseudoplusia includens (Leptidoptera: Noctuidae) [J]. J Invertehr Patho, 46: 70–82.
Chapman M S, G R M. 1993. Structure, sequence, and function correlations among parvoviruses [J]. Virology, 194 (11): 491–508.
Cotmore S F, Tattersall P. 1987. The autonomously replicating parvoviruses of vertebrates[J]. Adv Virus Res, 33: 91–174.
Dumas B, Jourdan M, Pascaud A M, et al. 1992. Complete nucleotide sequence of the cloned infectious genome of Junonia coenia densovirus reveals an organization unique among parvoviruses[J]. Virology, 191(1): 202–222.
El-Far M, Li Y, Fediere G, et al. 2004. Lack of infection of vertebrate cells by the densovirus from the maize worm Mythimna loreyi (MlDNV)[J]. Virus Res, 99(1): 17–24.
Fédière G. 2000. Epidemiology and pathology of Densovirinae[M]. Faisst S, Rommelaere J, ed. Parvoviruses. From mo-lecular biology to pathology and therapeutic uses. Karger: Basel, Switzerland. 1–11.
Fédière G, El-Far M, Li Y. 2004. Expression strategy of den-sonucleosis virus from Mythimna loreyi [J]. Virology, 320: 181–189.
Fédière G, Li Y, Zádori Z. 2002. Genome organization of Casphalia extranea densovirus, a new Iteravirus [J]. Virology, 292: 299–308.
Jiang H, Zhang J M, Wang J P, et al. 2006. Genetic engi-neering of Periplaneta fuliginosa densovirus as an improved biopesticide[J]. Arch Virol, 152: 383–394.
Jousset F X, Jourdan M, Compagnon B, et al. 1990. Restriction maps and sequence homologies of two densovirus genomes[J]. J Gen Virol, 71(10): 2463–2466.
Kelly D C, Bud H M. 1978. Densonucleosis virus DNA: analysis of fine structure by electron microscopy and agarose gel electrophoresis [J]. J Virol, 40: 33–43.
Kelly D C, Moore N F, Spilling C R, et al. 1980. Densonucleosis Virus Structural Proteins[J]. J Virol, 36(1): 224–235.
Kurstak E, Belloncik S, Brailovsky C. 1969. Transformation of mouse L cells by an invertebrate virus: densonucleosis virus (DNV)[J]. C R Acad Sci Hebd Seances Acad Sci D, 269(17): 1716–1719.(Fre)
Li Y, Zádori Z, Bando H. 2001. Genome organization of the densovirus from Bombyx mori (BmDNV-1) and enzyme activity of its capsid [J]. J Gen Virol, 82: 2821–2825.
Macedo N, Botelho P S M, PA VAN O H O. 1989. Utilisation d’insecticides viraux et de regulateurs de croissance pour le contr le de Diatraea saccharalis (Fabr., 1794) par voie aerienne[R].Proc. 20th /SSC/’Congr, October 12–21,Sao Paulo (Brazil).
Macedo N, Bothelho P S M. 1986. Ten years of biological control of D. saccharalis by Apanteles flavipes, in Sao Paulo state (Brazil)[R]. Congress Society Sugar Cane Technologists, August 21–31,1986, Jakarta, 551–562.
Margarido L A C, Castilho H J. 1988. Determination du niveau de dommages economiques du foreur de la canne a sucre, Diatraea saccharalis, pour les distilleries d’alcool[J]. Bra-zil Acurarero, 106: 41–46.
Meynadier G, Galichet P F, Veyrunes J C. 1977. Mise en evidence d’une densonucleose chez Diatraea saccharalis (Lep. Pyralidae)[J]. Entomophaga, 22: 115–120.
Meynardier G, Vago C, Plantevin G. 1964. Virose d’un type inhabituel chez le lépidoptère Galleria mellonella L [J]. Rev Zool Agric Appl, 63: 207–209.
Nakagaki M, Kawase S. 1980. Structural proteins of densonucleosis virus isolated from the silkworm Bomhyx mori infected with the flacherie virus[J]. J Invertebr Pathol, 36: 166–171.
Poitout S., Bues R. 1970. Elevage de plusieurs espèces de lépidoptères noctuidae sur le milieu artificiel riche et sur milieu artificiel simplifié [J]. Ann Zool Anim, 2: 79–91.
Samulski R J, Chang L S, Shenk T. 1989. Helper-free stocks of recombinant adeno-associated viruses: normal integration does not require viral gene expression[J]. J Virol, 63(9): 3822–3828.
Shike H, Dhar A K, Burns J C, et al. 2000. Infectious hypodermal and hematopoietic necrosis virus of shrimp is related to mosquito brevidensoviruses[J]. Virology, 277(1): 167–177.
Snyder R O, Samulski R J, Muzyczka N. 1990. In vitro re-solution of covalently joined AAV chromosome ends[J]. Cell, 60(1): 105–113.
Tattersall P. 2006. The evolution of parvovirus taxonomy[M]. Kerr J R ed. Parvoriruses. Landon: Hodder Arnold, 5–14.
Tijssen P, Bando H, Li Y, et al. 2006. Evolution of Densoviruses[M]. Kerr J R ed. Parvoriruses. Landon: Hodder Arnold, 55–66.
Tijssen P, Bergoin M. 1995. Densonucleosis viruses constitute an increasingly diversified subfamily among the parvoviruses [J]. Semin Virology, 6: 347–355.
Tijssen P, Li Y, El-Far M, et al. 2003. Organization and expression strategy of the ambisense genome of densonucleosis virus of Galleria mellonella[J]. J Virol, 77(19): 10357–10365.
Tijssen P, van den Hurk J, Kurstak E. 1976. Biochemical, biophysical, and biological properties of densonucleosis virus. I. Structural proteins [J]. J Virol, 17: 686–691.
Vago C, Duthoit J L, Delahaye F. 1966. Les lésions nucléaires de “virose a noyaux denses” du lepidoptere Galleria mellonella [J]. Arch Ges Virusforsch, 18: 344–349.
van Regenmortel M H V, Fauquet C M, Bishop D H L. 2000. Virus taxonomy: classification and nomenclature of viruses [M]. The seventh report of the International Committee on Taxonomy of Viruses. San Diego: Academic Press.
Foundation item: National Natural Science Foundation of China (30670081); Granted by IRD (Institute de Recherche pour developpement)
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Kouassi, N., Peng, Jx., Li, Y. et al. Pathogenicity of diatraea saccharalis densovirus to host insets and characterization of its viral genome. Virol. Sin. 22, 53–60 (2007). https://doi.org/10.1007/s12250-007-0062-8
- Diatraea saccharalis
- Genomic DNA
- Restriction map