Journal of Pharmaceutical Innovation

, Volume 9, Issue 1, pp 48–52 | Cite as

Optical Analysis of Zinc Oxide Quantum Dots with Bovine Serum Albumin and Bovine Hemoglobin

  • Rizwan WahabEmail author
  • Sourabh Dwivedi
  • Mohd Shahnawaz Khan
  • Abdulrahman M. Al-Senaidy
  • Hyung-Shik Shin
  • Javed Musarrat
  • Abdulaziz A. Al-Khedhairy
Research Article


Quantum dots (QDs) are widely used in medical, industrial, and household applications owing to their excellent biological property. For its wide medical application, the biocompatibility of QDs is an important aspect of research. The aim of the present study was to synthesize zinc oxide quantum dots (ZnO-QDs) and to investigate the interaction with bovine serum albumin (BSA) and bovine hemoglobin (BHb) using fluorescence quenching method and circular dichroism (CD). The study suggests that the electrostatic force of attraction favors the adsorption of BSA onto ZnO-QDs. The fluorescence quenching of BSA and BHb using QD indicates the formation of QDs-BSA complexes. The CD spectra also showed the changes in secondary structure of proteins by interacting with QDs.


Quantum dots Bovine serum albumin Bovine hemoglobin Circular dichroism 



Rizwan Wahab would like to extend appreciation to the Deanship of Scientific Research at King Saud University for funding the work through the research group project no. RGP-VPP-218.


  1. 1.
    Dykman L, Khlebtsov N. Gold nanoparticles in biomedical applications: recent advances and perspectives. Chem Soc Rev. 2012;41:2256–82.PubMedCrossRefGoogle Scholar
  2. 2.
    Llevot A, Astruc D. Applications of vectorized gold nanoparticles to the diagno sis and therapy of cancer. Chem Soc Rev. 2012;41:242–57.PubMedCrossRefGoogle Scholar
  3. 3.
    Rana S, Bajaj A, Mout R, Rotello VM. Monolayer coated gold nanoparticles for delivery applications. Adv Drug Deliv Rev. 2012;64:200–16.PubMedCentralPubMedCrossRefGoogle Scholar
  4. 4.
    Wahab R, Khan F, Musarrat J, Al-Khedhairy AA. Role of smart nanostructured materials in cancers, responsive materials and methods. U.S.A: Wiley-Scrivener; 2013. p. 237–72. Chapter 9.CrossRefGoogle Scholar
  5. 5.
    Wahab R, Kim YS, Hwang IH, Shin HS. A non aqueous synthesis, characterization of zinc oxide nanoparticles and its interaction with DNA. Synth Met. 2009;159(23–24):2443–52.CrossRefGoogle Scholar
  6. 6.
    Peters T. Serum albumin. Adv Protein Chem. 1985;37:161–245.PubMedCrossRefGoogle Scholar
  7. 7.
    Scheller FW, Bistolas N, Liu S, Jänchen M, Katterle M, Wollenberger U. Thirty years of haemoglobin electrochemistry. Adv Colloid Interf. 2005;116:111–20.CrossRefGoogle Scholar
  8. 8.
    Wahab R, Ansari SG, Kim YS, Seo HK, Shin HS. Room temperature synthesis of needle-shaped ZnO nanorods via sonochemical method. 2007; 253(18):7622–7626.Google Scholar
  9. 9.
    Chipman DM, Grisaro V, Sharon N. The binding of oligosaccharides containing IV-acetylglucosamine and IV-acetylmuramic acid to lysozyme. J Biol Chem. 1967;242:4388–94.PubMedGoogle Scholar
  10. 10.
    Lakowicz JR. Principles of fluorescence spectroscopy, 3rd ed., Springer 278–282, 2006.Google Scholar
  11. 11.
    Dwivedi S, Saquib Q, Al-Khedhairy AA, Musarrat J. Butachlor induced dissipation of mitochondrial membrane potential, oxidative DNA damage and necrosis in human peripheral blood mononuclear cells. Toxicology. 2012;302:77–87.PubMedCrossRefGoogle Scholar
  12. 12.
    Cullity BD. Elements of X-ray diffraction. MA: Addison-Wesley; 1978.Google Scholar
  13. 13.
    Hu YJ, Liu Y, Shen XS, Fang XY, Qu SS. Studies on the interaction between 1-hexylcarbamoyl-5-fluorouracil and bovine serum albumin. J Mol Struct. 2005;738:143–7.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Rizwan Wahab
    • 1
    Email author
  • Sourabh Dwivedi
    • 1
  • Mohd Shahnawaz Khan
    • 2
  • Abdulrahman M. Al-Senaidy
    • 2
  • Hyung-Shik Shin
    • 3
  • Javed Musarrat
    • 4
  • Abdulaziz A. Al-Khedhairy
    • 1
  1. 1.Department of Zoology, College of ScienceKing Saud UniversityRiyadhSaudi Arabia
  2. 2.Department of Biochemistry, College of ScienceKing Saud UniversityRiyadhSaudi Arabia
  3. 3.Energy Materials and Surface Science Laboratory, Solar Research Center, School of Chemical EngineeringChonbuk National UniversityJeonjuRepublic of Korea
  4. 4.Department of Agricultural Microbiology, Faculty of Agricultural SciencesAMUAligarhIndia

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