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Estuaries and Coasts

, Volume 36, Issue 1, pp 174–191 | Cite as

Seasonal Prevalence of Hematodinium sp. Infections of Blue Crabs in Three South Carolina (USA) Rivers

  • Kirk J. Parmenter
  • Patrick A. Vigueira
  • C. Kaighn Morlok
  • Jennifer A. Micklewright
  • Kylie M. Smith
  • Kimberly S. Paul
  • Michael J. Childress
Article

Abstract

Blue crab, Callinectes sapidus, commercial landings in the USA have been declining at an alarming rate. In South Carolina, these declines are significantly correlated with years of decreased rainfall and elevated salt marsh salinity. Previous studies suggest that higher salinity increases the risk of infection by Hematodinium sp., a dinoflagellate parasite of blue crabs, C. sapidus. A 4-year survey (June 2008 to March 2012) of blue crabs in the ACE Basin National Estuarine Research Reserve documented (1) the temporal and spatial patterns of Hematodinium sp. infection in relation to salinity, (2) some environmental correlates of disease prevalence, and (3) the characteristics of infected blue crabs. Sampling was conducted four times a year in March, June, September, and December in the South Edisto, Ashepoo, and Combahee rivers beginning in June 2008. Crab hemolymph samples were collected and preserved and DNA was successfully amplified for 2,303 individuals. Hematodinium sp. infection was evaluated by PCR amplification of its 18S rRNA gene and adjacent regions. Prevalence was highest in December 2008 in the Combahee River at sites closest to St. Helena Sound. The spatial and temporal pattern of Hematodinium sp. infection was correlated with several environmental parameters. Infected crabs exhibited differences in carapace shape and body condition compared to uninfected crabs. Overall, these results suggest that blue crabs in regions of higher salinity are at greater risk of infection by Hematodinium sp. and infected individuals exhibit sub-lethal effects of the disease.

Keywords

Hematodinium sp. Blue crab Salinity Infection pattern Morphology 

Notes

Acknowledgments

We would like to thank Margaret Ptacek, Tim Jordan, Joe Bisesi, and Aaron Lauritson. We thank Daniel Barrineau and Dr. Al Segars for assistance with logistics and use of the McKinzie Field Station. Karrie Brinkley and Dr. Dick Lee provided valuable reference samples and guidance during development of our PCR protocols. Kristine Moody and Dr. Margaret Ptacek graciously shared their time, expertise, and laboratory equipment. This field work was conducted with the permission of the ACE Basin National Estuarine Research Reserve and the SC Department of Natural Resources (permit # EX09-0004, EX10-0076, and EX11-0068). This article is a result of work sponsored by the South Carolina Sea Grant Consortium with support from NOAA National Sea Grant College Program, U.S. Department of Commerce, Grant No. 2010-R/CF 15 (MJC). Student participation was supported by a Clemson University Creative Inquiry Grant (MJC), a Calhoun Honors Research Grant (CKM and JAM), and a Wade Stackhouse Graduate Fellowship (KJP). This research was conducted under an award from the Estuarine Reserves Division, Office of Ocean and Coastal Resource Management, National Ocean Service, National Oceanic and Atmospheric Administration.

References

  1. Atlantic States Marine Fisheries Commission. 2004. Status of the blue crab (Callinectes sapidus) along the Atlantic coast. Special scientific report no. 80.Google Scholar
  2. Childress, M.J. 2010. Modeling the impact of drought on South Carolina blue crabs using a spatially-explicit individual-based population model. SC Water Resources Conference Proceedings, Columbia, SC.Google Scholar
  3. Frischer, M.E., R.F. Lee, M.A. Sheppard, A. Mauer, F. Rambow, M. Neumann, J.E. Brofft, T. Wizenmann, and J.M. Danforth. 2006. Evidence for a free-living life stage of the blue crab parasitic dinoflagellate, Hematodinium sp. Harmful Algae 5: 548–557.CrossRefGoogle Scholar
  4. Gruebl, T., M.E. Frischer, M. Sheppard, M. Neumann, A.N. Maurer, and R.F. Lee. 2002. Development of an 18S rRNA gene-targeted PCR-based diagnostic for the blue crab parasite Hematodinium sp. Diseases of Aquatic Organisms 49: 61–70.CrossRefGoogle Scholar
  5. Lee, R.F., and M.E. Frischer. 2004. The decline of the blue crab. American Scientist 92: 548–553.Google Scholar
  6. Li, C., J.D. Shields, T.L. Miller, H.J. Small, K.M. Pagenkopp, and K.S. Reece. 2010. Detection and quantification of the free-living stage of the parasitic dinoflagellate Hematodinium sp. in laboratory and environmental samples. Harmful Algae 9: 515–521.CrossRefGoogle Scholar
  7. Messick, G.A. 1994. Hematodinium perezi infections in adult and juvenile blue crabs Callinectes sapidus from coastal bays of Maryland and Virginia, USA. Diseases of Aquatic Organisms 19: 77–84.CrossRefGoogle Scholar
  8. Messick, G.A., and J.D. Shields. 2000. Epizootiology of the parasitic dinoflagellate Hematodinium sp. in the American blue crab Callinectes sapidus. Diseases of Aquatic Organisms 43: 139–152.CrossRefGoogle Scholar
  9. Messick, G.A., S.J. Jordan, and W.F. Van Heukelem. 1999. Salinity and temperature effects on Hematodinium sp. in the blue crab Callinectes sapidus. Journal of Shellfish Research 18: 657–662.Google Scholar
  10. Parmenter, K.J. 2012. The effects of drought on the abundance of the blue crab, Callinectes sapidus, in the ACE Basin NERR in South Carolina. PhD dissertation. Clemson University.Google Scholar
  11. Sheppard, M.A., A. Walker, M.E. Frischer, and R.F. Lee. 2003. Histopathology and prevalence of the parasitic dinoflagellate, Hematodinium sp, in crabs (Callinectes sapidus, Callinectes similis, Neopanope sayi, Libinia emarginata, Menippe mercenaria) from a Georgia estuary. Journal of Shellfish Research 22: 873–880.Google Scholar
  12. Shields, J.D., and C.M. Squyars. 2000. Mortality and hematology of blue crabs, Callinectes sapidus, experimentally infected with the parasitic dinoflagellate Hematodinium perezi. Fisheries Bulletin 98: 139–152.Google Scholar
  13. Small, H.J., J.D. Shields, K.L. Hudson, and K.S. Reece. 2007. Molecular detection of Hematodinium sp. infecting the blue crab, Callinectes sapidus. Journal of Shellfish Research 26: 131–139.CrossRefGoogle Scholar
  14. Stentiford, G.D., and J.D. Shields. 2005. A review of the parasitic dinoflagellate Hematodinium species and Hematodinium-like infections in marine crustaceans. Diseases of Aquatic Organisms 66: 47–70.CrossRefGoogle Scholar
  15. Whitaker, J.D., L.B. DeLancy, J.E. Jenkins, and M.B. Maddox. 1998. A review of the fishery and biology of the blue crab, Callinectes sapidus, in South Carolina. Journal of Shellfish Research 17: 459–463.Google Scholar
  16. Wilber, D.H. 1994. The influence of Apalachicola River flows on blue crab, Callinectus sapidus, in north Florida. Fisheries Bulletin 92: 180–188.Google Scholar
  17. Zohar, Y., A.H. Hines, O. Zmora, E.G. Johnson, R.N. Lipcius, R.D. Seitz, D.B. Eggleston, A.R. Place, E.J. Schott, J.D. Stubblefield, and J.S. Chung. 2008. The Chesapeake Bay blue crab (Callinectes sapidus): A multidisciplinary approach to responsible stock replenishment. Reviews in Fisheries Science 16: 24–34.CrossRefGoogle Scholar

Copyright information

© Coastal and Estuarine Research Federation 2012

Authors and Affiliations

  • Kirk J. Parmenter
    • 1
  • Patrick A. Vigueira
    • 1
  • C. Kaighn Morlok
    • 1
  • Jennifer A. Micklewright
    • 1
  • Kylie M. Smith
    • 1
  • Kimberly S. Paul
    • 1
  • Michael J. Childress
    • 1
  1. 1.Department of Biological SciencesClemson UniversityClemsonUSA

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