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Mapping Phytoplankton Community Physiology on a River Impacted Continental Shelf: Testing a Multifaceted Approach

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Abstract

Seasonal phosphorus limitation occurs on the Louisiana continental shelf as a result of high nitrogen loads in the spring and early summer. Prior studies have assessed such nutrient limitation by laborious and time-consuming nutrient analyses, enzyme assays, and nutrient addition bioassays. We undertook surface (0.5–1 m) mapping of fast repetition rate fluorescence (FRRF) parameters to assess nutrient limitation in real time on the Louisiana continental shelf and Mississippi River plume from 29 June to 08 July, 2002 in an effort to further understand phytoplankton productivity in this region, as well as to better inform effective nutrient management strategies. Surface nutrient concentrations (NO −3 , NO −2 , NH +4 , PO 3−4 ), chlorophyll a biomass, alkaline phosphatase (AP) activity, and four FRRF parameters: the maximum quantum yield of photochemistry (F v /F m ), the functional absorption cross section for PSII, the time constant for Q A reoxidation, and the connectivity factor, were measured during continuous underway mapping. Results from traditional methods to assess phytoplankton nutrient stress indicated widespread phosphorus limitation from the Mississippi River plume to the Atchafalaya River, manifested as high inorganic N/P ratios and elevated AP activities associated with phytoplankton biomass. The FRRF data indicated complex patterns of phytoplankton physiology that were likely driven by the rapidly changing conditions in local surface waters and heterogeneous phytoplankton community structure. Correlations of nutrient data and enzyme assays with FRRF parameters were significant but low, potentially due to differences in the manner and time scale with which nutrient limitation affects the different techniques used, indicating that further work is needed to interpret FRRF parameters in large, heterogeneous environments such as estuaries and continental shelves.

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Acknowledgments

We kindly thank F. Natale for running the Fast Repetition Rate fluorometer during the cruise, PG Falkowski for the use of his FRR fluorometer, R. Lauck for running the nutrient samples, and the crew of the R/V Pelican. This work was supported by National Science Foundation grant OCE 0196175 and National Oceanographic and Atmospheric Administration grant NA03NOS4780041 to JWA.

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Author notes

  1. Jason B. Sylvan

    Present address: Department of Biological Sciences, Marine Environmental Biology Section, University of Southern California, 3616 Trousdale Parkway, Los Angeles, CA, 90089-0371, USA

  2. Antonietta Quigg

    Present address: Department of Marine Biology, Texas A&M University at Galveston, 200 Seawolf Parkway, Galveston, TX, 77553, USA

  3. Sasha Tozzi

    Present address: Ocean Sciences, University of California, Santa Cruz, CA, 95064, USA

  4. James W. Ammerman

    Present address: School of Marine and Atmospheric Sciences, Stony Brook University, Stony Brook, NY, 11794-5000, USA

Authors and Affiliations

  1. Institute of Marine and Coastal Sciences, Rutgers University, 71 Dudley Road, New Brunswick, NJ, 08540, USA

    Jason B. Sylvan, Antonietta Quigg, Sasha Tozzi & James W. Ammerman

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  1. Jason B. Sylvan
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Correspondence to Jason B. Sylvan.

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Sylvan, J.B., Quigg, A., Tozzi, S. et al. Mapping Phytoplankton Community Physiology on a River Impacted Continental Shelf: Testing a Multifaceted Approach. Estuaries and Coasts 34, 1220–1233 (2011). https://doi.org/10.1007/s12237-011-9415-5

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