Skip to main content

Assessment of Wild Solanum Species for Resistance to Phytophthora infestans (Mont.) de Bary in the Toluca Valley, Mexico

American Journal of Potato Research volume 99, pages 25–39 (2022)Cite this article

Abstract

Ninety-seven accessions of 14 wild Solanum species were evaluated for late blight resistance under natural infection conditions in the Toluca Valley, México, for two consecutive growing seasons. Significant effects were detected for the interaction of Accession/Year variation in Disease Maximum, sAUDPC (standardized AUDPC), r (rate of disease increase per unit time), and T50 (time to reach 50% of the disease). Continuous variation in Disease Maximum, sAUDPCs, r, and T50 indicated the presence of unique or multiple strong R genes for disease resistance. Eleven accessions from seven species with a high degree of resistance (R) were identified. Seven accessions from six species were also moderately resistant (MR) in both years. Five accessions of three species were MR or R in just one year, with 23 accessions being of interest as potential sources of resistance. Solanum genotypes that have a wide range of resistance could be used in breeding programs and for molecular genetic studies.

Resumen

Catorce especies silvestres de Solanum se evaluaron para la resistencia al tizón tardío en condiciones de infección natural en el Valle de Toluca, México, durante dos ciclos de cultivo consecutivos. Se detectaron efectos significativos para la interacción de la variación Accesión/Año en El Máximo de la Enfermedad, sAUDPC (AUDPC estandarizado), r (tasa de aumento de la enfermedad por unidad de tiempo) y T 50 (tiempo para alcanzar el 50% de la enfermedad). La variación continua en el Máximo de la Enfermedad, sAUDPCs, r, y T 50 indicaron la presencia de genes R fuertes únicos o múltiples para la resistencia a la enfermedad. Se identificaron once accesiones de siete especies con un alto grado de resistencia (R). Siete accesiones de seis especies también fueron moderadamente resistentes (MR) en ambos años. Cinco accesiones de tres especies fueron MR o R en solo un año, con 23 accesiones de interés como fuentes potenciales de resistencia. Los genotipos de Solanum que tienen una amplia gama de resistencia podrían usarse en programas de mejoramiento y para estudios genéticos moleculares.

This is a preview of subscription content, access via your institution.

Access options

Buy single article

Instant access to the full article PDF.

USD 39.95

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Fig. 1
Fig. 2

References

  • Alfaro, R.C. 1995. Por los caminos evolutivos de la papa silvestre y cultivada. Ciencia & Desarrollo 1: 86–91.

    Google Scholar 

  • Bachmann-Pfabe, S., T. Hammann, J. Kruse, and K.J. Dehmer. 2019. Screening of wild potato genetic resources for combined resistance to late blight on tubers and pale potato cyst nematodes. Euphytica 215 (3): 1–15.

    CAS  Google Scholar 

  • Belmar-Díaz, C.R., and H. Lozoya-Saldaña. 2013. Incidencia de razas fisiológicas y genotipos de Phytophthora infestans en el valle de Toluca, México. Revista Chapingo Serie Horticultura 19 (2): 173–181.

    Google Scholar 

  • Busse, J.S., S.H. Jansky, H.I. Agha, C.A. Schmitz Carley, L.M. Shannon, and P.C. Bethke. 2021. A high throughput method for generating Dihaploids from tetraploid potato. American Journal of Potato Research: 1–11.

  • Carstensen, B., M. Plummer, E. Laara, and M. Hills. (2021). Epi: A package for statistical analysis in epidemiology. R package version 2.43, https://CRAN.R-project.org/package=Epi. Accessed 21 Aug 2021.

  • Champouret, N., K. Bouwmeester, H. Rietman, T. van der Lee, C. Maliepaard, A. Heupink, and V.G. Vleeshouwers. 2009. Phytophthora infestans isolates lacking class I ipiO variants are virulent on Rpi-blb1 potato. Molecular Plant-Microbe Interactions 22 (12): 1535–1545.

    CAS  PubMed  Google Scholar 

  • Danan, S., J.B. Veyrieras, and V. Lefebvre. 2011. Construction of a potato consensus map and QTL meta-analysis offer new insights into the genetic architecture of late blight resistance and plant maturity traits. BMC Plant Biology 11 (1): 1–17.

    Google Scholar 

  • Deahl, K.L., M.E. Gallegly, and R.J. Young. 1974. Laboratory testing of potato tubers for multigenic resistance to late blight. American Potato Journal 51 (10): 324–329.

    Google Scholar 

  • Diaz de la Cruz, J.B., H. Lozoya-Saldaña, J. Sahagún-Castellanos, and A. Peña-Lomelí. 2014. The Pathosystem Solanum tuberosum L.-Phytophthora infestans (Mont.) de Bary in Chapingo, Mexico. Expected, Observed, and Simulated. American Journal of Potato Research 91 (3): 312–326.

    Google Scholar 

  • Elansky, S., V.P. Apryshko, D.I. Milyutina, and B.E. Kozlovsky. 2007. Resistance of russian strains of Phytophthora infestans to fungicides metalaxyl and dimethomorph. Moscou University Biological Science Bulletin 62 (1): 14–18.

    Google Scholar 

  • Fernández-Pavía, S.P., N.J. Grünwald, and W.E. Fry. 2002. Formation of Phytophthora infestans oospores in nature on tubers in Central Mexico. Plant Disease 86 (1): 73–73.

    PubMed  Google Scholar 

  • Fernández-Pavía, S.P., N.J. Grünwald, M. Diaz-Valasis, M. Cadena-Hinojosa, and W.E. Fry. 2004. Soilborne oospores of Phytophthora infestans in Central Mexico survive winter fallow and infect potato plants in the field. Plant Disease 88 (1): 29–33.

    PubMed  Google Scholar 

  • Flier, W.G., N.J. Grünwald, L.P. Kroon, A.K. Sturbaum, T.B. van den Bosch, E. Garay-Serrano, and L.J. Turkensteen. 2003. The population structure of Phytophthora infestans from the Toluca Valley of Central Mexico suggests genetic differentiation between populations from cultivated potato and wild Solanum spp. Phytopathology 93 (4): 382–390.

    PubMed  Google Scholar 

  • Flores-Crespo, R. (1969). Taxonomía, distribución y potencial de los Solanum tuberíferos silvestres de México. Instituto Nacional de Investigaciones Agrícolas, SAG. Folleto Misceláneo no. 20. México.

  • Fry, W.E. 1978. Quantification of general resistance of potato cultivars and fungicide effects for integrated control of potato late blight. Phytopathology 68 (11): 1650–1655.

    CAS  Google Scholar 

  • Fry, W.E., P.R.J. Birch, H.S. Judelson, N.J. Grünwald, G. Danies, K.L. Everts, and C.D. Smart. 2015a. Five reasons to consider Phytophthora infestans a reemerging pathogen. Phytopathology 105 (7): 966–981.

    CAS  PubMed  Google Scholar 

  • Fry, B., G. Danies, I. Small, and K. Myers. (2015b). Recent developments concerning the population biology and control strategies of Phytophthora infestans in the USA. PPO-Special Report no. 17, 45.

  • Ghislain, M., A.A. Byarugaba, E. Magembe, A. Njoroge, C. Rivera, M.L. Román, and A. Kiggundu. 2019. Stacking three late blight resistance genes from wild species directly into African highland potato varieties confers complete field resistance to local blight races. Plant Biotechnology Journal 17 (6): 1119–1129.

    CAS  PubMed  Google Scholar 

  • Goodwin, S. B., L.J. Spielman, J. M. Matuszak, S. N. Bergeron, and W. E. Fry. (1992). Clonal diversity and genetic differentiation of Phytophthora infestans populations in northern and Central Mexico. Phytopathology (USA).

  • Gopal, J., and B.P. Singh. 2003. Screening potatoes for resistance to late blight (Phytophthora infestans) under field conditions. Potato Research 46 (1-2): 47–56.

    Google Scholar 

  • Grønbech, J., B. Andersson, L. Sjöholm, E. Liljeroth, E. Edin, R. Bain, and P. Vanhaverbeke. (2015). Epidemics and control of early and late blight, 2013 and 2014 in Europe. In: Proceedings of the fifteenth EuroBlight workshop, Brasov, Romania, 13-15 May 2015/Ed. HTAM Schepers. Applied Plant Research, Wageningen UR.

  • Grünwald, N.J. 2020. Using genomic approaches to inform management of emerging plant pathogens. Revista Mexicana de Fitopatología 38: S8 Supplement 2020.

    Google Scholar 

  • Grünwald, N.J., and W.G. Flier. 2005. The biology of Phytophthora infestans at its center of origin. Annual Review of Phytopathology 43: 171–190.

    PubMed  Google Scholar 

  • Grünwald, N.J., W.G. Flier, A.K. Sturbaum, E. Garay-Serrano, T.B. van den Bosch, C.D. Smart, and W.E. Fry. 2001. Population structure of Phytophthora infestans in the Toluca valley region of Central Mexico. Phytopathology 91 (9): 882–890.

    PubMed  Google Scholar 

  • Haesaert, G., J.H. Vossen, R. Custers, M. De Loose, A. Haverkort, B. Heremans, and G. Gheysen. 2015. Transformation of the potato variety Desiree with single or multiple resistance genes increases resistance to late blight under field conditions. Crop Protection 77: 163–175.

    CAS  Google Scholar 

  • Haverkort, A.J., P.M. Boonekamp, R. Hutten, E. Jacobsen, L.A.P. Lotz, G.J.T. Kessel, and E.A.G. Van der Vossen. 2008. Societal costs of late blight in potato and prospects of durable resistance through cisgenic modification. Potato Research 51 (1): 47–57.

    Google Scholar 

  • Haverkort, A.J., P.M. Boonekamp, R. Hutten, E. Jacobsen, L.A.P. Lotz, G.J.T. Kessel, and R.G.F. Visser. 2016. Durable late blight resistance in potato through dynamic varieties obtained by cisgenesis: Scientific and societal advances in the DuRPh project. Potato Research 59 (1): 35–66.

    CAS  Google Scholar 

  • Henfling, J.W. (1987). El tizón tardío de la papa: Phytophthora infestans. II Edición. Boletín de Información Técnica no. 4. CIP. Lima, Perú.

  • Hougas, R.W., and S.J. Peloquin. 1958. The potential of potato haploids in breeding and genetic research. American Journal of Potato 35: 701–707.

    Google Scholar 

  • Huang, S., V.G. Vleeshouwers, R.G. Visser, and E. Jacobsen. 2005. An accurate in vitro assay for high-throughput disease testing of Phytophthora infestans in potato. Plant Disease 89 (12): 1263–1267.

    PubMed  Google Scholar 

  • Karki, H.S., S.H. Jansky, and D.A. Halterman. 2020. Screening of wild potatoes identifies new sources of late blight resistance. Plant Disease. https://doi.org/10.1094/PDIS-06-20-1367-RE.

  • Khiutti, A.L.E.X., D.M. Spooner, S.H. Jansky, and D.A. Halterman. 2015. Testing taxonomic predictivity of foliar and tuber resistance to Phytophthora infestans in wild relatives of potato. Phytopathology 105 (9): 1198–1205.

    CAS  PubMed  Google Scholar 

  • Lal, M., S.K. Luthra, B.P. Singh, and S. Yadav. 2013. Screening of genotypes against potato late blight. Potato Journal 40 (1): 80–83.

    Google Scholar 

  • Lindqvist-Kreuze, H., S. Gamboa, M. Izarra, W. Pérez, M.Y. Correa, A. Astete, and P. Gonzáles. 2020. Population structure and host range of the potato late blight pathogen Phytophthora infestans in Peru spanning two decades. Plant Pathology 69 (2): 334–346.

    Google Scholar 

  • Lozoya-Saldaña, H. 2005. Importancia Internacional del Valle de Toluca, México, en Estudios Sobre el Tizón Tardío de la Papa (Solanum tuberosum L.), Causado Por Phytophthora infestans (Mont.) de Bary. Revista Mexicana de Fitopatología 23 (3): 312–319.

    Google Scholar 

  • Lozoya-Saldana, H. 2011. Evolution of vertical and horizontal resistance and its application in breeding resistance to potato late blight. Potato Journal 38 (1): 1–8.

    Google Scholar 

  • Lozoya-Saldaña, H., and A. Hernández-Vilchis. 2001. Postharvest decay of potato tubers in moist chamber and in two types of soil. Revista Mexicana de Fitopatología 19 (2): 140–146.

    Google Scholar 

  • Lozoya-Saldaña, H., M.H. Coyote-Palma, R. Ferrera-Cerrato, and M.E. Lara-Hernández. 2006. Antagonismo microbiano contra Phytophthora infestans (Mont) de Bary. Agrociencia 40 (4): 491–499.

    Google Scholar 

  • Micheletto, S., R. Boland, and M. Huarte. 2000. Argentinian wild diploid Solanum species as sources of quantitative late blight resistance. Theoretical and Applied Genetics 101 (5-6): 902–906.

    Google Scholar 

  • Pankin, A.A., E.A. Sokolova, E.V. Rogozina, M.A. Kuznetsova, K.L. Deahl, R.W. Jones, and E.E. Khavkin. 2010. Searching among wild Solanum species for homologues of RB/Rpi-blb1 gene conferring durable late blight resistance. PPO-Special Report 14: 277–284.

    Google Scholar 

  • Pankin, A., E. Sokolova, E. Rogozina, M. Kuznetsova, K. Deahl, R. Jones, and E. Khavkin. 2011. Allele mining in the gene pool of wild Solanum species for homologues of late blight resistance gene RB/Rpi-blb1. Plant Genetic Resources 9 (2): 305–308.

    CAS  Google Scholar 

  • Pérez, W., A. Salas, R. Raymundo, Z. Huaman, R. Nelson, and M. Bonierbale. (2000). Evaluation of wild potato species for resistance to late blight. Scientist and farmer, Partners in Research for the 21st century, Program Report 1999–2000, 49-62.

  • Rakosy-Tican, E., R. Thieme, J. König, M. Nachtigall, T. Hammann, T.E. Denes, and M. Molnár-Láng. 2020. Introgression of two broad-Spectrum late blight resistance genes, Rpi-Blb1 and Rpi-Blb3, from Solanum bulbocastanum dun plus race-specific R genes into potato pre-breeding lines. Frontiers in Plant Science 11: 699.

    PubMed  PubMed Central  Google Scholar 

  • Raman, K.V., N.J. Grünwald, and W.E. Fry. 2000. Promoting international collaboration for potato late blight disease management. Pesticide Outlook 11 (5): 181–185.

    Google Scholar 

  • Robledo-Esqueda, M.N., H. Lozoya-Saldaña, and M.T. Colinas-León. 2012. Inducción de defensa en papa (Solanum tuberosum L.) contra Phytophthora infestans Mont. de bar y por fungicidas. Interciencia 37 (9): 689–695.

    Google Scholar 

  • Romero-Montes, G., H. Lozoya-Saldaña, G. Mora-Aguilera, S. Fernández-Pavía, and N.J. Grünwald. 2012. Rendimiento de papa en función de epidemia por tizón tardío (Phytophthora infestans Mont. de Bary). Revista Fitotecnia Mexicana 35 (1): 69–78.

    Google Scholar 

  • Santa, J.D., J. Berdugo-Cely, L. Cely-Pardo, M. Soto-Suárez, T. Mosquera, and C. H. Galeano M. 2018. QTL analysis reveals quantitative resistant loci for Phytophthora infestans and Tecia solanivora in tetraploid potato (Solanum tuberosum L.). PLoS One 13 (7): e0199716.

    PubMed  PubMed Central  Google Scholar 

  • Shakya, S.K., M.M. Larsen, M.M. Cuenca-Condoy, H. Lozoya-Saldaña, and N.J. Grünwald. 2018. Variation in genetic diversity of Phytophthora infestans populations in Mexico from the center of origin outwards. Plant Disease 102 (8): 1534–1540.

    PubMed  Google Scholar 

  • Shanner, G., and R.E. Finney. 1977. The effect of nitrogen fertilization on the expression of slow mildewing resistance in Knox wheat. Phytopathology 67: 1057–1066.

    Google Scholar 

  • Sieber, K., G. M. Forster, A. Berger, T. Hammann, A. Kellermann, and A. Schwarzfischer, 2015. Assessment of genetic hotspots for Phytophthora resistance and their use as molecular markers in potato breeding. PPO-Special Report no. 17, 57.

  • Song, J., J.M. Bradeen, S.K. Naess, J.A. Raasch, S.M. Wielgus, G.T. Haberlach, and J. Jiang. 2003. Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight. Proceedings of the National Academy of Sciences 100 (16): 9128–9133.

    CAS  Google Scholar 

  • Spooner, D.M., S. Jansky, F. Rodríguez, R. Simon, M. Ames, D. Fajardo, and R.O. Castillo. 2019. Taxonomy of wild potatoes in northern South America (Solanum section Petota). Systematic Botany Monographs 108: 1–305.

    Google Scholar 

  • Tiwari, J.K., S. Siddappa, B.P. Singh, S.K. Kaushik, S.K. Chakrabarti, V. Bhardwaj, and P. Chandel. 2013. Molecular markers for late blight resistance breeding of potato: An update. Plant Breeding 132 (3): 237–245.

    CAS  Google Scholar 

  • Tiwari, J.K., S. Devi, S. Sharma, P. Chandel, S. Rawat, and S., and B. P. Singh. 2015. Allele mining in Solanum germplasm: Cloning and characterization of RB-homologous gene fragments from late blight resistant wild potato species. Plant Molecular Biology Reporter 33 (5): 1584–1598.

    CAS  Google Scholar 

  • Turner, R.S. 2005. After the famine: Plant pathology, Phytophthora infestans, and the late blight of potatoes, 1845––1960. Historical Studies in the Physical and Biological Sciences 35 (2): 341–370.

    Google Scholar 

  • Vanderplank, J.E. 1982. Host-pathogen interactions in plant disease, 143–177. New York: Elsevier.

    Google Scholar 

  • Vleeshouwers, V.G., H. Rietman, P. Krenek, N. Champouret, C. Young, S.K. Oh, and E.A. Van der Vossen. 2008. Effector genomics accelerates discovery and functional profiling of potato disease resistance and Phytophthora infestans avirulence genes. PLoS One 3 (8): e2875.

    PubMed  PubMed Central  Google Scholar 

  • Vossen, J.H., G. van Arkel, M. Bergervoet, K.R. Jo, E. Jacobsen, and R.G. Visser. 2016. The Solanum demissum R8 late blight resistance gene is an Sw-5 homologue that has been deployed worldwide in late blight resistant varieties. Theoretical and Applied Genetics 129 (9): 1785–1796.

    CAS  PubMed  Google Scholar 

  • Wang, M., S. Allefs, R.G. van den Berg, V.G. Vleeshouwers, E.A. van der Vossen, and B. Vosman. 2008. Allele mining in Solanum: Conserved homologues of Rpi-blb1 are identified in Solanum stoloniferum. Theoretical and Applied Genetics 116 (7): 933–943.

    CAS  PubMed  Google Scholar 

  • Wang, J., S.P. Fernández-Pavía, M.M. Larsen, E. Garay-Serrano, R. Gregorio-Cipriano, G. Rodríguez-Alvarado, N.J. Grünwald, and E.M. Goss. 2017. High levels of diversity and population structure in the potato late blight pathogen at the Mexico Centre of origin. Molecular Ecology 26: 1091–1107. https://doi.org/10.1111/mec.14000.

    Article  PubMed  Google Scholar 

  • Wastie, R.L. 1991. Breeding for resistance. In Advances in plant pathology 7: Phytophthora infestans, the cause of late blight of potato, ed. D.S. Ingram and P.H. Williams, 193–223. New York: Academic Press.

    Google Scholar 

  • Whitworth, J.L., R.G. Novy, J.J. Pavec, D.L. Corsini, H. Lozoya-Saldaña, S. Yilma, A.R. Mosley, and I.M. Vales. 2007. Multiple site identification of potato parent clones conferring high levels of late blight resistance with a corresponding genetic model for resistance. American Journal of Potato Research 84 (4): 313–321.

    Google Scholar 

  • Zoteyeva, N.M. 2020. Late blight resistance of wild potato species under field conditions in the northwest of Russia. Proceedings on Applied Botany, Genetics and Breeding 180 (4): 159–169.

    Google Scholar 

  • Zoteyeva, N., M. Chrzanowska, B. Flis, and E. Zimnoch-Guzowska. 2012. Resistance to pathogens of the potato accessions from the collection of NI Vavilov Institute of Plant Industry (VIR). American Journal of Potato Research 89 (4): 277–293.

    Google Scholar 

Download references

Acknowledgments

Partial financial support was received from Special Projects in International Programs, Department of Global Development, School of Integrative Plant Science, Plant Breeding and Genetics Section, Cornell University, Ithaca, NY, USA. The authors also acknowledge and thank the Mexican National Council of Science and Technology (CONACYT), the US Potato Genebank-NRSP-6, Sutrgeon Bay, WI, and the State of México Agricultural Research and Training Institute (ICAMEX), for supporting this research as part of the first author’s Ph.D. dissertation.

Author information

Authors and Affiliations

  1. Instituto de Horticultura, Universidad Autónoma Chapingo, Carretera México-Texcoco Km 38.5, C. P. 56230, Chapingo, Estado de México, Mexico

    Guillermo Andrés Enciso-Maldonado, Héctor Lozoya-Saldaña, María Teresa Colinas-Leon & Jesús Axayacatl Cuevas-Sanchez

  2. Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, NC, 27695, USA

    Andres Dejesús Sanabria-Velázquez

  3. US Potato Genebank NRSP-6, USDA, Sturgeon Bay, WI, USA

    John Bamberg

  4. Special Projects in International Programs, Department of Global Development, School of Integrative Plant Science, Plant Breeding and Genetics Section, Cornell University, Ithaca, NY, USA

    K. V. Raman

Authors
  1. Guillermo Andrés Enciso-Maldonado
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Héctor Lozoya-Saldaña
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. María Teresa Colinas-Leon
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jesús Axayacatl Cuevas-Sanchez
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Andres Dejesús Sanabria-Velázquez
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. John Bamberg
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. K. V. Raman
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Héctor Lozoya-Saldaña.

Ethics declarations

Conflict of Interest

The authors declare no conflict of interest.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Enciso-Maldonado, G.A., Lozoya-Saldaña, H., Colinas-Leon, M.T. et al. Assessment of Wild Solanum Species for Resistance to Phytophthora infestans (Mont.) de Bary in the Toluca Valley, Mexico. Am. J. Potato Res. 99, 25–39 (2022). https://doi.org/10.1007/s12230-021-09856-x

Download citation

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12230-021-09856-x

Keywords

  • Late blight
  • Breeding
  • Disease resistance