Abstract
Methods for diploid true potato seed (TPS) production and handing are crucial for inbred-hybrid potato breeding. This study determined the effects of mother plant nutrition on average TPS weight and germination kinetics as modeled by Gompertz curves. Additionally, it characterized germination of stratified TPS and TPS imbibed in water or gibberellic acid (GA3). Seeds were produced by three interspecific hybrids between diploid relatives of cultivated potato and diploid lines derived from cultivated potato. Effects of stratification on seed dormancy and germination rate were genotype-dependent. With one exception, GA3-treated seeds reached 50% germination as fast or faster than stratified seeds and seeds imbibed in water. Mother plant nutrition was varied by fertilizing with two nutrient solutions that differed in composition. Effects of fertilizer type on germination were modest, and 100-seed weight did not differ between fertilizer types. These findings support previous research showing that GA3 treatment effectively promotes robust TPS germination.
Resumen
Los métodos para la producción y manejo de semillas de papa verdadera diploides (TPS) son cruciales para el mejoramiento de papa híbrida endogámica. Este estudio determinó los efectos de la nutrición de las plantas madre en el peso promedio de TPS y la cinética de germinación según lo modelado por las curvas de Gompertz. Además, caracterizó la germinación de TPS estratificada y TPS embebidas en agua o en ácido giberelico (GA 3). Las semillas se produjeron por tres híbridos interespecíficos entre parientes diploides de papa cultivada y líneas diploides derivadas de la papa cultivada. Los efectos de la estratificación en la latencia de las semillas y la tasa de germinación dependieron del genotipo. Con una excepción, las semillas tratadas con GA 3 alcanzaron el 50% de germinación tan rápido o más rápido que las semillas estratificadas y que las que se embebieron en agua. La nutrición de las plantas madre varió al fertilizar con dos soluciones nutritivas que difirieron en composición. Los efectos del tipo de fertilizante en la germinación fueron modestos, y el peso de 100 semillas no difirió entre los tipos de fertilizantes. Estos hallazgos apoyan investigaciones anteriores que muestran que el tratamiento GA 3 promueve eficazmente la germinación robusta de TPS.
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Funding
Partial funding for this research was provided by USDA NIFA Specialty Crop Research Initiative grant (2019–51181–30021). Additional funding was provided by the Japan Public–Private Partnership Student Study Abroad Program (TOBITATE).
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Nashiki, A., Jansky, S.H. & Bethke, P.C. The Effect of Mother Plant Fertilization and Stratification on the Germination of True Potato Seed. Am. J. Potato Res. 98, 194–201 (2021). https://doi.org/10.1007/s12230-021-09830-7
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DOI: https://doi.org/10.1007/s12230-021-09830-7