Expression of the Tomato pot-1 Gene Confers Potato Virus Y (PVY) Resistance in Susceptible Potato Varieties

Abstract

The translation initiation factor 4E (eIF4E) has been implicated in naturally-occurring resistance to the Potato Virus Y (PVY) determined by the pot-1 locus in tomato. The susceptible potato varieties Russet Norkotah, Silverton Russet, and Classic Russet, along with the Michigan State University breeding line MSE149-5Y, were transformed with the pot-1 gene from Solanum hirsutum to achieve PVY resistance. Transgenic potato events and their non-transgenic controls were screened for PVY resistance against three PVY strains (PVYO, PVYN:O, PVYNTN) by enzyme-linked immunosorbent assay (ELISA) in greenhouse and in field trials. The event A95.87.05 (Classic Russet) and MSE149.87.22 (MSE149-5Y) had resistance against all three PVY isolates tested in both foliage and tuber under either greenhouse or field conditions. In contrast, SR.87.62 (Silverton Russet) and RN1.87.09 (Russet Norkotah), displayed moderate susceptibility to PVY in foliage during the growing season and increased level of infection in tubers. Transformation of PVY-susceptible potato varieties with the tomato pot-1 gene can be a useful tool for improving PVY resistance.

Resumen

El factor de iniciación de traducción 4E (elF4E) se ha implicado en resistencia de ocurrencia natural al Virus Y de la Papa (PVY), determinado por el locus pot-1 en tomate. Las variedades susceptibles de papa Russet Norkotah, Silverton Russet y Classic Russet, junto con la línea de mejoramiento de la Universidad Estatal de Michigan MSE149-5Y, se transformaron con el gen pot-1 de Solanum hirsutum para lograr la resistencia al PVY. Los eventos de la papa transgénica y sus testigos no trangénicos se evaluaron para la resistencia a tres variantes del PVY (PVYO, PVY N:O, y PVY NTN) mediante serología de enzimas conjugadas (ELISA) en ensayos de invernadero y campo. Los eventos A95.87.05 (Classic Russet) y MSE149.87.22 (MSE149-5Y) tuvieron resistencia contra los tres aislamientos de PVY probados, tanto en follaje como en tubérculo, ya sea bajo condiciones de invernadero o de campo. En contraste, SR.87.62 (Silverton Russet) y RN1.87.09 (Russet Norkotah), mostraron susceptibilidad moderada al PVY en follaje durante el ciclo de cultivo y aumentaron el nivel de infección en tubérculos. La transformación de variedades de papa susceptibles al PVY con el gen pot-1 de tomate puede ser una herramienta útil para mejorar la resistencia al PVY.

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Acknowledgements

This work was supported by the Michigan Potato Industry Commission.

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Correspondence to David S. Douches.

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Zhang, C., Zarka, K.A., Zarka, D.G. et al. Expression of the Tomato pot-1 Gene Confers Potato Virus Y (PVY) Resistance in Susceptible Potato Varieties. Am. J. Potato Res. 98, 42–50 (2021). https://doi.org/10.1007/s12230-020-09815-y

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Keywords

  • Potato virus Y
  • Pot-1
  • Recessive resistance
  • eIF4E
  • Copy number variation