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Mapping Solanum berthaultii-based Late Blight (Phytophthora infestans) Resistance in a Diploid Population

Abstract

Late blight, caused by the oomycete Phytophthora infestans (Mont) de Bary, is the most important disease of cultivated potato (Solanum tuberosum L.). An efficient long-term strategy for controlling late blight infection involves integration of host plant resistance to disease management programs. In this study, a diploid potato population segregating for late blight resistance was generated from an interspecific cross (MSX902) between S. tuberosum x S. chacoense hybrid (84SD22) and a late blight resistant Solanum berthaultii hybrid (Ber83). A total of 129 progeny and two parents were tested for foliar resistance against the US-23 P. infestans genotype using detached-leaf bioassays and inoculated field trials (2014 and 2015). Distribution of foliar late blight resistance and susceptibility was bimodal in both types of phenotype evaluation, suggesting a major R-gene may be associated with the resistance. A major QTL for late blight resistance on chromosome 10 was detected using both detached leaf bioassay and field trial data. The SNP marker solcap_snp_c1_15219 (26.5 cM) was at the peak of the QTL. Additionally, a minor QTL on chromosomes 5 was linked to SNPs solcap_snp_c1_3793. These SNP markers closely linked to the late blight resistance QTL can be used for marker-assisted breeding.

Resumen

El tizón tardío de la papa, causado por el oomiceto Phytophthora infestans (Mont) de Bary, es la enfermedad mas importante de la papa cultivada (Solanum tuberosum L.). Una estrategia eficiente a largo plazo para controlar la infección del tizón tardío involucra la integración de la resistencia de la planta hospedante a programas de manejo a de la enfermedad. En este estudio, se generó una población diploide de papa segregante para resistencia al tizón tardío de una cruza interespecífica (MSX902) entre S. tuberosum x un híbrido de S. chacoense (84SD22) y un híbrido resistente al tizón tardío de Solanum berthaultii (Ber83). Se probaron un total de 129 progenies y dos progenitores para resistencia foliar al genotipo US-23 de P. infestans usando el bioensayo de hoja desprendida y ensayos de campo inoculados (2014 y 2015). La distribución de la resistencia foliar al tizón tardío y la susceptibilidad fue bimodal en ambos tipos de evaluación fenotípica, lo que sugiere que un gene R mayor pudiera estar asociado con la resistencia. Se detectó un QTL mayor para la resistencia al tizón tardío en el cromosoma 10 usando tanto los datos del bioensayo de hoja desprendida como los de ensayos de campo. El marcador SNP solcap_snp_c1_15219 (26.5 cM) estaba en la punta del QTL. Adicionalmente, se ligó a un QTL menor en el cromosoma 5 a SNPs solcap_snp_c1_3793. Estos marcadores SNP muy cercanamente ligados al QTL de resistencia al tizón tardío pueden usarse para el mejoramiento asistido con marcadores.

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References

  1. Ames, M., and D. M. Spooner. 2008. DNA from herbarium specimens settles a controversy about origins of the European potato. American Journal of Botany 95: 252–257.

    CAS  PubMed  Google Scholar 

  2. Ballvora, A., M. R. Ercolano, J. Weiß, K. Meksem, C. A. Bormann, P. Oberhagemann, F. Salamini, and C. Gebhardt. 2002. The R1 gene for potato resistance to late blight (Phytophthora infestans) belongs to the leucine zipper/NBS/LRR class of plant resistance genes. Plant Journal. 30: 361–371.

    CAS  PubMed  Google Scholar 

  3. Bisognin, D. A., D. S. Douches, L. Buszka, G. Bryan, and D. Wang. 2005. Mapping late blight resistance in Solanum microdontum Bitter. Crop Science. 45: 340–345.

    CAS  Google Scholar 

  4. Bisognin, D. A., N. C. Manrique-Carpintero, and D. S. Douches. 2018. QTL analysis of tuber dormancy and sprouting in potato QTL analysis of tuber dormancy and sprouting in potato. American Journal of Potato Research. 95: 374–382.

    CAS  Google Scholar 

  5. Black, W. 1951. Inheritance of Resistance to Blight (Phytophthora infestans) in Potatoes: Inter-Relationships of Genes and Strains. Proceedings of the Royal Society of Edinburgh. Section B. Biology. 64: 312–352.

  6. Brylińska, M., I. Tomczyńska, H. Jakuczun, I. Wasilewicz-Flis, K. Witek, J. D. Jones, and J. Śliwka. 2015. Fine mapping of the Rpi-rzc1 gene conferring broad-spectrum resistance to potato late blight. European Journal of Plant Pathology. 143: 193–198.

    Google Scholar 

  7. Chakrabarti, S. K., B. P. Singh, G. Thakur, J. K. Tiwari, S. K. Kaushik, S. Sharma, and V. Bhardwaj. 2014. QTL Analysis of late blight resistance in a diploid potato family of Solanum spegazzinii × S. chacoense. Potato Research 57: 1–11.

    Google Scholar 

  8. Chen, X., D. Lewandowska, M. R. Armstrong, K. Baker, T. Lim, M. Bayer, B. Harrower, K. McLean, F. Jupe, K. Witek, A. K. Lees, J. D. Jones, G. J. Bryan, and I. Hein. 2018. Identification and rapid mapping of a gene conferring broad-spectrum late blight resistance in the diploid potato species Solanum verrucosum through DNA capture technologies. Theoretical and Applied Genetics. 131: 1287–1297. https://doi.org/10.1007/s00122-018-3078-6.

    CAS  Article  PubMed  Google Scholar 

  9. Collins, A., D. Milbourne, L. Ramsay, R. Meyer, C. Chatot-Balandras, P. Oberhagemann, W. De Jong, C. Gebhardt, E. Bonnel, and R. Waugh. 1999. QTL for field resistance to late blight in potato are strongly correlated with maturity and vigour. Molecular Breeding. 5: 387–398.

    CAS  Google Scholar 

  10. Colon, L. T., L. J. Turkensteen, W. Prummel, D. J. Budding, and J. Hoogendoorn. 1995. Durable resistance to late blight cultivars (Phytophthora infestans) in old potato. European Journal of Plant Pathology. 101: 387–397.

    Google Scholar 

  11. Costanzo, S., I. Simko, B. J. Christ, and K. G. Haynes. 2005. QTL analysis of late blight resistance in a diploid potato family of Solanum phureja x S. stenotomum. Theoretical and Applied Genetics. 111: 609–617.

    CAS  PubMed  Google Scholar 

  12. Danan, S., J. E. Chauvin, B. Caromel, J. D. Moal, R. Pellé, and V. Lefebvre. 2009. Major-effect QTLs for stem and foliage resistance to late blight in the wild potato relatives Solanum sparsipilum and S. spegazzinii are mapped to chromosome X. Theoretical and Applied Genetics 119: 705–719.

    CAS  PubMed  Google Scholar 

  13. Dangi, S., N. Rosenzweig, L. Steere, and W. W. Kirk. 2016. First Report of late blight caused by Phytophthora infestans clonal lineage US-23 variant on potato in Michigan, USA. Plant Pathology. https://doi.org/10.1094/PDIS-02-16-0228-PDN.

  14. De Jong, W., A. Forsyth, D. Leister, C. Gebhardt, and D.C. Baulcombe. 1997. A potato hypersensitive resistance gene against potato virus X maps to a resistance gene cluster on chromosome 5. Theoretical and Applied Genetics. 95: 246–252.

    Google Scholar 

  15. Douches, D. S., and C. F. Quiros. 1987. Use of 4x-2x crosses to determine gene-centromere map distances of isozyme loci in Solanum species. Genome. 29: 5l9–527.

    Google Scholar 

  16. Douches, D. S., J. B. Bamberg, W. W. Kirk, K. Jastrzebski, B. A. Niemira, J. Coombs, D. A. Bisognin, and K. J. Felcher. 2001. Evaluation of wild Solanum species for resistance to the US-8 genotype of Phytophthora infestans utilizing a fine-screening technique. American Journal of Potato Research. 78: 159–165.

    Google Scholar 

  17. Douches, D.S., K. Zarka, K. Hokanson, M. Ghislain, P.S. Wharton, J. Medendorp, C.M. Richael, and N. Champouret. 2019. USAID feed the future biotechnology potato partnership: 2018 update. American Journal of Potato Research. 96: 329. https://doi.org/10.1007/s12230-019-09723-w.

    CAS  Article  Google Scholar 

  18. El-Kharbotly, A., C. Palomino-Sánchez, F. Salamini, E. Jacobsen, and C. Gebhardt. 1996. R6 and R7 alleles of potato conferring race-specific resistance to Phytophthora infestans (Mont.) de Bary identified genetic loci clustering with the R3 locus on chromosome XI. Theoretical and Applied Genetics. 92: 880–884.

    CAS  PubMed  Google Scholar 

  19. Ewing, E. E., I. Šimko, C. D. Smart, M. W. Bonierbale, S. G. Eduardo, G. D. May, and W. E. Fry. 2000. Genetic mapping from field tests of qualitative and quantitative resistance to Phytophthora infestans in a population derived from Solanum tuberosum and Solanum berthaultii. Molecular Breeding. 6: 25–36.

    CAS  Google Scholar 

  20. FAOSTAT. 2020. FAOSTAT database collections. Food and Agriculture Organization of the United Nations. http://www.fao.org/faostat/. Accessed 24 Aug 2020.

  21. Forbes, G. A., and J. T. Korva. 1994. The effect of using a Horsfall-Barratt scale on precision and accuracy of visual estimation of potato late blight severity in the field. Plant Pathology. 43: 675–682. https://doi.org/10.1111/j.1365-3059.1994.tb01606.x.

    Article  Google Scholar 

  22. Frisch, M., and A.E. Melchinger. 2005. Selection theory for marker-assisted backcrossing. Genetics 170: 909–917.

    CAS  PubMed  PubMed Central  Google Scholar 

  23. Ghislain, M., B. Trognitz, Ma. del R. Herrera, J. Solis, G. Casallo, C. Vásquez, O. Hurtado, R. Castillo, L. Portal, and M. Orrillo. 2001. Genetic loci associated with field resistance to late blight in offspring of Solanum phureja and S. tuberosum grown under short-day conditions. Theoretical and Applied Genetics. 103: 433–442.

    CAS  Google Scholar 

  24. Golas, T. M., H. Geest, J. Gros, A. Sikkema, N. D’Agostino, J. P. Nap, C. Mariani, J. J. H. M. Allefs, and I. Rieu. 2013. Comparative next-generation mapping of the Phytophthora infestans resistance gene Rpi-dlc2 in a European accession of Solanum dulcamara. Theoretical and Applied Genetics. 126: 59–68.

    CAS  PubMed  Google Scholar 

  25. Haverkort, A. J., P. C. Struik, R. G. F. Visser, and E. Jacobsen. 2009. Applied biotechnology to combat late blight in potato aused by Phytophthora Infestans. Potato Research. 52: 249–264.

    Google Scholar 

  26. Hawkes, J. G. 1958. Significance of wild species and primitive forms for potato breeding. Euphytica. 7: 257–270.

    Google Scholar 

  27. Helgeson, J. P., J. D. Pohlman, S. Austin, G. T. Haberlach, S. M. Wielgus, D. Ronis, L. Zambolim, P. Tooley, J. M. McGrath, R. V. James, and W. R. Stevenson. 1998. Somatic hybrids between Solanum bulbocastanum and potato: a new source of resistance to late blight. Theoretical and Applied Genetics. 96: 738–742.

    Google Scholar 

  28. Kim, H. J., H. R. Lee, K. R. Jo, S. M. M. Mortazavian, D. J. Huigen, B. Evenhuis, G. Kessel, R. G. F. Visser, E. Jacobsen, and J. H. Vossen. 2012. Broad spectrum late blight resistance in potato differential set plants MaR8 and MaR9 is conferred by multiple stacked R genes. Theoretical and Applied Genetics. 124: 923–935.

    CAS  PubMed  Google Scholar 

  29. Kirk, W. W., B. A. Niemira, J. M. Stein, and R. L. Schafer. 1999. Late blight development from potato seed-pieces treated with fungicides. Pesticide Science. 55: 1151–1158.

    CAS  Google Scholar 

  30. Kirk, W. W., K. J. Felcher, D. S. Douches, J. Coombs, J. M. Stein, K. M. Baker, and R. Hammerschmidt. 2001. Effect of host plant resistance and reduced rates and frequencies of fungicide application to control potato late blight. Plant Disease 85: 1113–1118.

    CAS  PubMed  Google Scholar 

  31. Kreike, C. M., J. R. A. de Koning, J. H. Vinke, J. W. van Ooijen, and W. J. Stiekema. 1994. Quantitatively-inherited resistance to Globodera pallida is dominated by one major locus in Solanum spegazzinii. Theoretical and Applied Genetics. 88: 764–769.

    CAS  PubMed  Google Scholar 

  32. Kuhl, J. C., R. E. Hanneman Jr., and M. J. Havey. 2001. Characterization of and mapping of Rpi1, a late-blight resistance locus from diploid (1EBN) Mexican Solanum pinnatisectum. Molecular Genetics and Genomics. 265: 977–985.

    CAS  PubMed  Google Scholar 

  33. Leesutthiphonchai, W., A. L. Vu, A. M. V. Ah-Fong, and H. S. Judelson. 2018. How does Phytophthora infestans evade control efforts? Modern insight into the late blight disease. Phytopathology. 108: 916–924.

    CAS  PubMed  Google Scholar 

  34. Lindhout, P., M. de Vries, M. ter Maat, S. Ying, M. Viquez-Zamora, and S. van Heusden. 2018. Hybrid potato breeding for improved varieties. https://doi.org/10.19103/AS.2016.0016.04.

  35. Malcolmson, J. F., and W. Black. 1966. New R genes in Solanum demissum Lindl. and their complementary races of Phytophthora infestans (Mont.) de bary. Euphytica. 15: 199–203.

    Google Scholar 

  36. Manrique-Carpintero, N.C., J.J. Coombs, R.E. Veilleux, C.R. Buell, and D.S. Douches. 2016. Comparative analysis of regions with distorted segregation in three diploid populations of potato. G3: Genes Genomes, Genetics. 6: 2617–2628.

    Google Scholar 

  37. Marcin, J. 2012. A resistance gene against potato late blight originating from Solanum michoacanum maps to potato chromosome VII. Theoretical and Applied Genetics. 124: 397–406.

    Google Scholar 

  38. Massa, A.N., N.C. Manrique-Carpintero, J.J. Coombs, D.G. Zarka, A.E. Boone, W.W. Kirk, C.A. Hackett, G.J. Bryan, and D.S. Douches. 2015. Genetic linkage mapping of economically important traits in cultivated tetraploid potato (Solanum tuberosum L.). . G3: Genes Genomes, Genetics. 5: 2357–2364.

    Google Scholar 

  39. Nachtigall, M., K. Janine, and R. Thieme. 2018. Mapping of a novel, major late blight resistance locus in the diploid (1EBN) Mexican Solanum pinnatisectum Dunal on chromosome VII. Plant Breeding. 137: 433–442.

    CAS  Google Scholar 

  40. Naess, S.K., J.M. Bradeen, S.M. Wielgus, G.T. Haberlach, J.M. McGrath, and J.P. Helgeson. 2000. Resistance to late blight in Solanum bulbocastanum is mapped to chromosome 8. Theoretical and Applied Genetics. 101: 697–704.

    CAS  Google Scholar 

  41. Nowicki, M., M.R. Foolad, M. Nowakowska, and E.U. Kozik. 2011. Potato and tomato late blight caused by Phytophthora infestans: An overview of pathology and resistance breeding. Plant Disease. 96: 4–17.

    Google Scholar 

  42. Park, T.H., J. Gros, A. Sikkema, V.G.A.A. Vleeshouwers, M. Muskens, S. Allefs, E. Jacobsen, R.G.F. Visser, and E.A.G. van der Vossen. 2005a. The late blight resistance locus Rpi-blb3 from Solanum bulbocastanum belongs to a major late blight R gene cluster on chromosome 4 of potato. Molecular Plant-Microbe Interactions. 18: 722–729.

    CAS  PubMed  Google Scholar 

  43. Park, T. H., V. G. A. A. Vleeshouwers, R. C. B. Hutten, H. J. Van Eck, E. van der Vossen, E. Jacobsen, and R. G. F. Visser. 2005b. High-resolution mapping and analysis of the resistance locus Rpi-abpt against Phytophthora infestans in potato. Molecular Breeding. 16: 33–43.

    CAS  Google Scholar 

  44. Park, T. H., S. Foster, G. Brigneti, and J. D. G. Jones. 2009. Two distinct potato late blight resistance genes from Solanum berthaultii are located on chromosome 10. Euphytica. 165: 269–278.

    Google Scholar 

  45. Pel, M.A., S.J. Foster, T.H. Park, H. Rietman, G. van Arkel, J.D.G. Jones, H.J. Van Eck, E. Jacobsen, R.G.F. Visser, and E.A.G. Van der Vossen. 2009. Mapping and cloning of late blight resistance genes from Solanum venturii using an interspecific candidate gene approach. Molecular Plant-Microbe Interactions. 22: 601–615.

    CAS  PubMed  Google Scholar 

  46. Pomerantz, A., Y. Cohen, E. Shufan, Y. Ben-Naim, S. Mordechai, A. Salman, and M. Huleihel. 2014. Characterization of Phytophthora infestans resistance to mefenoxam using FTIR spectroscopy. Journal of Photochemistry and Photobiology. 141: 308–314.

    CAS  Google Scholar 

  47. Raffaele, S., R. A. Farrer, L. M. Cano, D. J. Studholme, D. MacLean, M. Thines, R. H. Y. Jiang, M. C. .Zody, S. G. Kunjeti, N. M. Donofrio. B. C. Meyers, and S. Kamoun. 2010. Genome evolution following host jumps in the Irish Potato Famine pathogen lineage. Science 330: 1540–1543.

    CAS  PubMed  Google Scholar 

  48. Rauscher, G. M., C. D. Smart, I. Simko, M. Bonierbale, H. Mayton, A. Greenland, and W. E. Fry. 2006. Characterization and mapping of RPi-ber, a novel potato late blight resistance gene from Solanum berthaultii. Theoretical and Applied Genetics 112: 674–687.

    CAS  PubMed  Google Scholar 

  49. Ritter, E., T. Debener, A. Barone, F. Salamini, and C. Gebhardt. 1991. RFLP mapping on potato chromosomes of two genes controlling extreme resistance to potato virus X (PVX). Molecular and General Genetics. 227: 81–85.

    CAS  PubMed  Google Scholar 

  50. Sandbrink, J. M., L. T. Colon, P. C. Wolters, and W. J. Stiekema. 2000. Two related genotypes of Solanum microdontum carry different segregating alleles for field resistance to Phytophthora infestans. Molecular Breeding. 6: 215–225.

    CAS  Google Scholar 

  51. Simko, I. 2002. Comparative analysis of quantitative trait loci for foliage resistance to Phytophthora infestans in tuber-bearing Solanum species. American Journal of Potato Research. 79: 125–132.

    CAS  Google Scholar 

  52. Simko, I., S. Costanzo, V. Ramanjulu, B. J. Christ, and K. G. Haynes. 2006. Mapping polygenes for tuber resistance to late blight in a diploid Solanum phureja X S. stenotomum hybrid population. Plant Breeding. 125: 385–389.

    Google Scholar 

  53. Śliwka, J., H. Jakuczun, M. Chmielarz, A. Hara-Skrzypiec, I. Tomczyńska, A. Kilian, and E. Zimnoch-Guzowska. 2012. Late blight resistance gene from Solanum ruiz-ceballosii is located on potato chromosome X and linked to violet flower colour. BMC Genetics. https://doi.org/10.1186/1471-2156-13-11.

    Article  PubMed  PubMed Central  Google Scholar 

  54. Smart, C. D., and W. E. Fry. 2001. Invasions by the late blight pathogen: Renewed sex and enhanced fitness. Biological Invasions 3: 235–243.

    Google Scholar 

  55. Song, J., J. M. Bradeen, S. K. Naess, J. A. Raasch, S. M. Wielgus, G. T. Haberlach, J. Liu, H. Kuang, S. Austin-Phillips, C. R. Buell, J. P. Helgeson, and J. Jiang. 2003. Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight. Proceedings of the National Academy of Sciences of the United States of America 100: 9128–9133.

    CAS  PubMed  PubMed Central  Google Scholar 

  56. Tan, M. Y. A., R. C. B. Hutten, C. Celis, T. H. Park, R. E. Niks, R. G. F. Visser, and H. J. Van Eck. 2008. The RPi-mcd1 locus from Solanum microdontum involved in resistance to Phytophthora infestans, causing a delay in infection, maps on potato chromosome 4 in a cluster of NBS-LRR genes. Molecular Plant-Microbe Interactions 21: 909–918.

    CAS  PubMed  Google Scholar 

  57. Tan, M.Y.A., R.C.B. Hutten, R.G.F. Visser, and H.J. van Eck. 2010. The effect of pyramiding Phytophthora infestans resistance genes RPi-mcd1 and RPi-ber in potato. Theoretical and Applied Genetics. 121: 117–125.

    PubMed  Google Scholar 

  58. Trognitz, F., P. Manosalva, R. Gysin, D. Niño-Liu, R. Simon, M. del Rosario Herrera, B. Trognitz, M. Ghislain, and R. Nelson. 2002. Plant defense genes associated with quantitative resistance to potato late blight in Solanum phureja × Dihaploid S. tuberosum hybrids. Molecular Plant-Microbe Interactions 15: 587–597.

    CAS  PubMed  Google Scholar 

  59. Van Ooijen, J.W. 2006. JoinMap 4. Software for the calculation of genetic linkage maps in experimental populations. Wageningen: Kyazma B.V.

    Google Scholar 

  60. Van Ooijen, J.W. 2009. MapQTL 6, Software for the mapping of quantitative trait loci in experimental populations of dipoid species. Wageningen: Kyazma B.V.

    Google Scholar 

  61. Van Ooijen, J. W. 2011. Multipoint maximum likelihood mapping in a full-sib family of an outbreeding species. Genetics Research. 93: 343–349.

    Google Scholar 

  62. Van Ooijen, J.W., H. Sandbrink, C. Purimahua, R. Vrielink, R. Verkerk, P. Zabel, and P. Lindhout. 1993. Mapping quatitative genes involved in a trait assessed on an ordinal scale: a case study with bacterial canker in Lycopersicon peruvianum. In Molecular Biology of Tomato, ed. J.I. Yoder, 5–74. Lancaster: Technomic Publishing Co. Inc.

    Google Scholar 

  63. Villamon, F.G., D.M. Spooner, M. Orrillo, E. Mihovilovich, W. Pérez, and M. Bonierbale. 2005. Late blight resistance linkages in a novel cross of the wild potato species Solanum paucissectum (series Piurana). Theoretical and Applied Genetics. 111: 1201–1214.

    CAS  PubMed  Google Scholar 

  64. Visker, M. H. P. W., H. J. B. Heilersig, L. P. Kodde, W. E. Van de Weg, R. E. Voorrips, P. C. Struik, and L. T. Colon. 2005. Genetic linkage of QTLs for late blight resistance and foliage maturity type in six related potato progenies. Euphytica. 143: 189–199.

    CAS  Google Scholar 

  65. Vossen, E.A.G., J. Gros, A. Sikkema, M. Muskens, D. Wouters, P. Wolters, A. Pereiera, and S. Allefs. 2005. The Rpi-blb2 gene from Solanum bulbocastanum is an Mi-1 gene homolog conferring broad-spectrum late blight resistance in potato. The Plant Journal. 44: 208–222.

    PubMed  Google Scholar 

  66. Wan, D., R. Li, B. Zou, X. Zhang, J. Cong, R. Wang, and G.L. Yiji Xia. 2012. Calmodulin-binding protein CBP60g is a positive regulator of both disease resistance and drought tolerance in Arabidopsis. Plant Cell Reports. 31: 1269–1281.

    CAS  PubMed  Google Scholar 

  67. Yang, L., D. Wang, Y. Xu, H. Zhao, L. Wang, X. Cao, and Y. Chen. 2017. A new resistance gene against potato late blight originating from Solanum pinnatisectum located on potato chromosome 7. Frontiers in Plant Science. https://doi.org/10.3389/fpls.2017.01729.

  68. Zhu, S., Y. Li, J.H. Vossen, R.G.F. Visser, and E. Jacobsen. 2012. Functional stacking of three resistance genes against Phytophthora infestans in potato. Transgenic Research. 21: 89–99.

    CAS  PubMed  Google Scholar 

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Funding

This project was supported by USAID-Borlaug Higher Education for Agricultural Research and Development funding.

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Correspondence to David S. Douches.

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Manrique-Carpintero, N., Islam, M.S., Enciso-Rodríguez, F. et al. Mapping Solanum berthaultii-based Late Blight (Phytophthora infestans) Resistance in a Diploid Population. Am. J. Potato Res. 97, 596–604 (2020). https://doi.org/10.1007/s12230-020-09808-x

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Keywords

  • Late blight
  • Diploid breeding
  • Wild potato