Advertisement

American Journal of Potato Research

, Volume 94, Issue 4, pp 323–333 | Cite as

BC1 and F1 Progeny from Solanum × michoacanum (+) S. tuberosum Somatic Hybrids, Autofused 4× S. michoacanum and Cultivated Potato

  • Paulina Smyda-DajmundEmail author
  • Jadwiga Śliwka
  • Iwona Wasilewicz-Flis
  • Henryka Jakuczun
  • Ewa Zimnoch-Guzowska
Article

Abstract

Solanum × michoacanum (mch) is a valuable source of resistance to Phytophthora infestans and has not been used in potato breeding due to crossing barriers with S. tuberosum. Somatic hybridization followed by backcrossing is a strategy for introgression of important traits from wild potato species sexually isolated from S. tuberosum. Tetraploid somatic hybrids Solanum × michoacanum (+) S. tuberosum [mch (+) tbr] and autofused 4× mch lines were crossed to several potato cultivars as male and female parents. Our results indicate that resistance against late blight, originating from mch (+) tbr somatic hybrids and autofused 4× mch lines can be transferred to cultivated potato by sexual crossing. Viable and fertile progeny was obtained providing a route to using mch as a source of resistance to P. infestans in potato breeding.

Keywords

Backcrossing Phytophthora infestans Male fertility Resistance Sexual progeny Tuber morphology 

Abbreviations

mch (+) tbr

Solanum × michoacanum (+) S. tuberosum

4× mch

4× S. michoacanum

P. infestans

Phytophthora infestans

EBN

Endosperm Balance Number

BC1

First backcross generation hybrid

F1

First generation hybrid

Resumen

Solanum × michoacanum (mch) es una valiosa Fuente de Resistencia a Phytophthora infestans y no ha sido usada en el mejoramiento de la papa debido a las barreras de cruzamiento con S. tuberosum. La hibridación somática, seguida de retrocruzas, es una estrategia para introgresión de caracteres importantes de especies silvestres de papa aisladas sexualmente de S. tuberosum. Hibridos somáticos tetraploides de Solanum × michoacanum (+) S. tuberosum [mch (+) tbr] y líneas autofusionadas 4× mch se cruzaron con varias variedades de papa como progenitores machos y hembras. Nuestros resultados indican que la resistencia contra el tizón tardío, originada de híbridos somáticos mch (+) tbr y líneas autofusionadas 4× mch pudiera transferirse a papa cultivada mediante cruzas sexuales. Se obtuvieron progenies viables y fértiles y se puede continuar investigación posterior para explorar mch como fuente de resistencia a P. infestans en el mejoramiento de la papa.

Notes

Acknowledgements

This work was financed by the National Science Centre in Poland, grant: UMO-2012/07/B/NZ9/01901.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

References

  1. Camadro, E.L., D. Carputo, and S.J. Peloquin. 2004. Substitutes for genome differentiation in tuber-bearing Solanum: interspecific pollen-pistil incompatibility, nuclear-cytoplasmic male sterility, and endosperm. Theoretical and Applied Genetics 109: 1369–1376.CrossRefPubMedGoogle Scholar
  2. Carputo, D., L. Monti, J.E. Werner, and L. Frusciante. 1999. Uses and usefulness of endosperm balance number. Theoretical and Applied Genetics 98: 478–484.CrossRefGoogle Scholar
  3. Chandel, P., J.K. Tiwari, N. Ali, S. Devi, S. Sharma, S. Sharma, S.K. Luthra, and B.P. Singh. 2015. Interspecific potato somatic hybrids between Solanum tuberosum and S. cardiophyllum, potential sources of late blight resistance breeding. Plant Cell Tissue and Organ Culture 123: 579–589.CrossRefGoogle Scholar
  4. Domański, L. 2001. Assessment of morphological characters of potato tubers. Monografie i Rozprawy Naukowe IHAR, Radzików, Poland 10a:92–95.Google Scholar
  5. Dong, F., R.G. Novy, J.P. Helgeson, and J. Jiang. 1999. Cytological characterization of potato – Solanum etuberosum somatic hybrids and their backcross progenies by genomic in situ hybridization. Genome 42: 987–992.CrossRefGoogle Scholar
  6. Ehlenfeld, M.K., and J.P. Helgeson. 1987. Fertility of somatic hybrids from protoplast fusions of Solanum brevidens and S. tuberosum. Theoretical and Applied Genetics 73: 395–402.Google Scholar
  7. Gavrilenko, T., R. Thieme, U. Heimbach, and T. Thieme. 2003. Fertile somatic hybrids of Solanum etuberosum (+) dihaploid Solanum tuberosum and their backcrossing progenies: relationships of genome dosage with tuber development and resistance to potato virus Y. Euphytica 131: 323–332.CrossRefGoogle Scholar
  8. Hawkes, J.G. 1990. The potato, evolution, 82. London: Biodiversity and Genetic Resources. Belhaven Press.Google Scholar
  9. Helgeson, J.P., G.T. Haberlach, M.K. Ehlenfeldt, G. Hunt, J.D. Pohlman, and S. Austin. 1993. Sexual progeny of somatic hybrids between potato and Solanum brevidens: potential for use in breeding programs. American Journal of Potato Research 70: 437–452.CrossRefGoogle Scholar
  10. Helgeson, J.P., D. Pohlman, S. Austin, G.T. Haberlach, S.M. Wielgus, D. Ronis, L. Zambolim, P. Tooley, J.M. McGrath, R.V. James, and W.R. Stevenson. 1998. Somatic hybrids between Solanum bulbocastanum and potato: a new source of resistance to late blight. Theoretical and Applied Genetics 96: 738–742.CrossRefGoogle Scholar
  11. Horsman, K. 2001. Somatic hybrids of Solanum tuberosum and species of the Solanum nigrum – complex and their backcross progeny. Wageningen: PhD thesis, Wageningen University.Google Scholar
  12. Horsman, K., J.E.M. Bergervoet, and E. Jacobsen. 1997. Somatic hybridization between Solanum tuberosum and species of the S. nigrum Complex: selection of vigorously growing and flowering plants. Euphytica 96: 345–352.CrossRefGoogle Scholar
  13. Horsman, K., R. Fratini, D. Huigen, and E. Jacobsen. 1999. Successful first and second backcrosses of S. nigrum (+) S. tuberosum somatic hybrids with both Solanum parents. Sexual Plant Reproduction 12: 144–151.CrossRefGoogle Scholar
  14. Horsman, K., T. Gavrilenko, M. Bergervoet, D.J. Huigen, A.T.W. Joe, and E. Jacobsen. 2001. Alteration of the genomic composition of Solanum nigrum (+) potato backcross derivatives by somatic hybridization: selection of fusion hybrids by DNA measurements and GISH. Plant Breeding 120: 201–207.CrossRefGoogle Scholar
  15. Jackson, S.A., and R.E. Hanneman Jr. 1999. Crossability between cultivated and wild tuber- and non-tuber-bearing solanums. Euphytica 109: 51–67.CrossRefGoogle Scholar
  16. Jacobsen, E., R. Malvar, D.J. Huigen, J.E.M. Bergervoet, and M.S. Ramanna. 1993. Isolation and characterization of somatic hybrids of diploid Solanum tuberosum and Solanum brevidens and the use of amylase-free starch mutation for detection of introgression. Euphytica 69: 191–201.CrossRefGoogle Scholar
  17. Jakuczun, H., and I. Wasilewicz-Flis. 2004. Ziemniak diploidalny źródłem cech jakościowych w hodowli. Zeszyty Problemowe Postepów Nauk Rolniczych 500: 127–136 (in Polish).Google Scholar
  18. Jansky, S. 2000. Breeding for disease resistance in potato. Plant Breeding Reviews 19: 69–155.Google Scholar
  19. Jansky, S. 2006. Overcoming hybridization barriers in potato. Plant Breeding 125: 1–12.CrossRefGoogle Scholar
  20. Janssen, A.W.B., and J.G.Th. Hermsen. 1976. Estimating pollen fertility in Solanum species and haploids. Euphytica 25: 577–586.CrossRefGoogle Scholar
  21. Johnston, S.A., T.M. den Nijs, J. Peloquin, and R.E. Hanneman. 1980. The significance of genetic balance to endosperm development in interspecific crosses. Theoretical and Applied Genetics 57: 5–9.CrossRefPubMedGoogle Scholar
  22. Kuhl, J.C., R.E. Hanneman, and M.J. Havey. 2001. Characterization and mapping of Rpi1, a late-blight resistance locus from diploid (1EBN) Mexican Solanum pinnatisectum. Molecular Genetics and Genomics 265: 977–985.CrossRefPubMedGoogle Scholar
  23. Nowacki, W., D. Boguszewska, Z. Czerko, W. Goliszewski, M. Grudzińska, J. Jankowska, B. Lutomirska, W. Nowacki, M. Pietraszko, C. Trawczyński, A. Wierzbicka, K. Zaczyńska, K. Zgórska, and K. Michalak. 2012. Charakterystyka krajowego rejestru odmian ziemniaka. Poland: Wydanie XV, Jadwisin 2012.Google Scholar
  24. Orczyk, W., J. Przetakiewicz, and A. Nadolska-Orczyk. 2003. Somatic hybrids of Solanum tuberosum – application to genetics and breeding. Plant Cell, Tissue and Organ Culture 74: 1–13.CrossRefGoogle Scholar
  25. Park, T.H., J. Gros, A. Sikkema, V.G.A. Vleeshouwers, M. Muskens, S. Allefs, E. Jacobsen, R.G.F. Visser, and E.A.G. van der Vossen. 2005a. The late blight resistance locus Rpi-blb3 from Solanum bulbocastanum belongs to a major late blight R gene cluster on chromosome 4 of potato. Molecular Plant-Microbe Interactions 18: 722–729.CrossRefPubMedGoogle Scholar
  26. Park, T.H., V.G.A. Vleeshouwers, R.C.B. Hutten, H.J. van Eck, E.A.G. van der Vossen, E. Jacobsen, and R.G.F. Visser. 2005b. High-resolution mapping and analysis of the resistance locus Rpi-abpt against Phytophthora infestans in potato. Molecular Breeding 16: 33–43.CrossRefGoogle Scholar
  27. Park, T.H., V.G.A.A. Vleeshouwers, E. Jacobsen, E. van der Vossen, and R.G.F. Visser. 2009. Molecular breeding for resistance to Phytophthora infestans (Mont.) de Bary in potato (Solanum tuberosum L.): a perspective of cisgenesis. Plant Breeding 128: 109–117.CrossRefGoogle Scholar
  28. Rakosy-Tican, E., T. Ramona, M. Nachtigall, I. Molnar, and R.-E. Denes. 2015. The recipient potato cultivar influences the genetic makeup of the somatic hybrids between five potato cultivars and one cloned accession of sexually incompatible species Solanum bulbocastanum dun. Plant Cell, Tissue and Organ Culture 122: 395–407.CrossRefGoogle Scholar
  29. Rodewald, J., and B. Trognitz. 2013. Solanum resistance genes against Phytophthora infestans and their corresponding avirulence genes. Molecular Plant Pathology 14: 740–757.CrossRefPubMedGoogle Scholar
  30. Rokka, V.-M., Y.-S. Xu, J. Kankila, A. Kuusela, S. Pulli, and E. Pehu. 1994. Identification of somatic hybrids of dihaploid Solanum tuberosum lines and S. brevidens by species specific RAPD patterns and assessment of disease resistance of the hybrids. Euphytica 80: 207–2017.CrossRefGoogle Scholar
  31. Rothacker, D.J., and W. Junges. 1966. Untersuchungen zur Erzeugung und Auslese dihaploider Sämlinge bei Solanum tuberosum L. European Potato Journal 9: 99–110.CrossRefGoogle Scholar
  32. Sarkar, D., J.K. Tiwari, S. Sharma, S. Poonam, J. Sharma, B. Gopal, P. Singh, S.K. Luthra, S.K. Pandey, and D. Pattanayak. 2011. Production and characterization of somatic hybrids between Solanum tuberosum L. and S. pinnatisectum dun. Plant Cell Tissue and Organ Culture 107: 427–440.CrossRefGoogle Scholar
  33. Śliwka, J., H. Jakuczun, M. Chmielarz, A. Hara-Skrzypiec, I. Tomczyńska, A. Kilian, and E. Zimnoch-Guzowska. 2012. A resistance gene against potato late blight originating from Solanum × michoacanum maps to potato chromosome VII. Theoretical and Applied Genetics 124: 397–406.CrossRefPubMedGoogle Scholar
  34. Smyda, P., H. Jakuczun, K. Dębski, J. Śliwka, R. Thieme, M. Nachtigall, I. Wasilewicz-Flis, and E. Zimnoch-Guzowska. 2013. Development of somatic hybrids S. × michoacanum bitter. (Rydb.) (+) S. tuberosum L. and autofused 4× S. × michoacanum plants as potential sources of late blight resistance for potato breeding. Plant Cell Reports 32: 1231–1241.CrossRefPubMedPubMedCentralGoogle Scholar
  35. Smyda-Dajmund, P., J. Śliwka, I. Wasilewicz-Flis, H. Jakuczun, and E. Zimnoch-Guzowska. 2016. Genetic composition of interspecific potato somatic hybrids and autofused 4× plants evaluated by DArT and cytoplasmic DNA markers. Plant Cell Reports 35: 1345–1358.CrossRefPubMedPubMedCentralGoogle Scholar
  36. Spooner, D.M., and J.B. Bamberg. 1994. Potato genetic resources: sources of resistance and systematic. American Potato Journal 71: 325–337.CrossRefGoogle Scholar
  37. Szczerbakowa, A., J. Tarwacka, M. Oskiera, H. Jakuczun, and B. Wielgat. 2010. Somatic hybridization between the diploids of S. × michoacanum and S. tuberosum. Acta Physiologiae Plantarum 32: 867–873.CrossRefGoogle Scholar
  38. Thieme, R., U. Darsow, T. Gavrilenko, D. Dorokhov, and H. Tiemann. 1997. Production of somatic hybrids between S. tuberosum L. and late blight resistant Mexican wild potato species. Euphytica 97: 189–200.CrossRefGoogle Scholar
  39. Thieme, R., U. Darsow, L. Rakosy-Tican, Z. Kang, T. Gavrilenko, O. Antonova, U. Heimbach, and T. Thieme. 2004. Use of somatic hybridization to transfer resistance to late blight and Potato Virus Y (PVY) into cultivated potato. Plant Breeding and Seed Science 50: 113–118.Google Scholar
  40. Thieme, R., E. Rakosy-Tican, T. Gavrilenko, O. Antonova, J. Schubert, M. Nachtigall, U. Heimbach, and T. Thieme. 2008. Novel somatic hybrids (Solanum tuberosum L. + Solanum tarnii) and their fertile BC1 progenies express extreme resistance to potato virus Y and late blight. Theoretical and Applied Genetics 116: 691–700.CrossRefPubMedGoogle Scholar
  41. Thieme, R., E. Rakosy-Tican, M. Nachtigall, J. Schubert, T. Hammann, O. Antonova, T. Gavrilenko, U. Heimbach, and T. Thieme. 2010. Characterization of the multiple resistance traits of somatic hybrids between Solanum cardiophyllum Lindl. And two commercial potato cultivars. Plant Cell Reports 29: 1187–1201.CrossRefPubMedGoogle Scholar
  42. Tiwari, J.K., P.D. Sarkar, S.K. Pandey, J. Gopal, and S.R. Kumar. 2010. Molecular and morphological characterization of somatic hybrids between Solanum tuberosum L. and S. etuberosum Lindl. Plant Cell Tissue and Organ Culture 103: 175–187.CrossRefGoogle Scholar
  43. van der Vossen, E., A. Sikkema, B.L. Hekkert, J. Gros, P. Stevens, M. Muskens, D. Wouters, A. Pereira, W. Stiekema, and S. Allefs. 2003. An ancient R gene from the wild potato species Solanum bulbocastanum confers broad-spectrum resistance to Phytophthora infestans in cultivated potato and tomato. The Plant Journal 36: 867–882.CrossRefPubMedGoogle Scholar
  44. van der Vossen, E., J. Gros, W. Stiekema, M. Muskens, D. Wouters, P. Wolters, A. Pereira, and S. Allefs. 2005. The Rpi-blb2 gene from Solanum bulbocastanum is an Mi-1 gene homolog conferring broad-spectrum late blight resistance in potato. The Plant Journal 44: 208–222.CrossRefPubMedGoogle Scholar
  45. Yamada, T., K. Hosaka, N. Kaide, K. Nakagawa, S. Misoo, and O. Kamijima. 1998. Cytological and molecular characterization of BC1 progeny from two somatic hybrids between dihaploid Solanum acaule and tetraploid S. tuberosum. Genome 41: 743–750.CrossRefGoogle Scholar
  46. Zoteyeva, N., M. Chrzanowska, B. Flis, and E. Zimnoch-Guzowska. 2012. Resistance to pathogens of the potato accessions from the collection of N.I. Vavilov Institute of Plant Industry (VIR). American Journal of Potato Research 89: 277–229.CrossRefGoogle Scholar

Copyright information

© The Potato Association of America 2017

Authors and Affiliations

  • Paulina Smyda-Dajmund
    • 1
    Email author
  • Jadwiga Śliwka
    • 1
  • Iwona Wasilewicz-Flis
    • 1
  • Henryka Jakuczun
    • 1
  • Ewa Zimnoch-Guzowska
    • 1
  1. 1.Plant Breeding and Acclimatization Institute - National Research Institute, Młochów Research CenterMłochówPoland

Personalised recommendations