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A Mutant eIF4E Confers Resistance to Potato Virus Y Strains and is Inherited in a Dominant Manner in the Potato Varieties Atlantic and Russet Norkotah

Abstract

Potato Virus Y (PVY) is one of the most important pathogens affecting potato production worldwide. Here we tested the efficacy of engineered resistance against PVY in two popular susceptible potato varieties, Russet Norkotah and Atlantic. This resistance is based on the expression of a modified potato eIF4E gene containing mutations homologous to those in the recessive resistant allelic variant found in pepper. The expression of this eIF4E variant conferred differential resistance responses against three prevalent PVY strains including PVYO, PVYN:O and PVYNTN in the five tested transgenic lines. When resistance was established, the virus was not detectable in the inoculated leaves, the newly emerged leaves, nor in the sprouted tubers. Some strains were restrained to a low but detectable level in some of the potato lines. Crosses between transformed and wild type lines demonstrated that the engineered resistance gene was inherited in a dominant manner.

Resumen

El virus Y de la papa (PVY) es uno de los patógenos más importantes que afectan la producción de la papa a nivel mundial. Aquí probamos la eficacia de resistencia asistida contra PVY en dos variedades susceptibles populares de papa, Russet Norkotah y Atlantic. Esta resistencia se basa en la expresión de un gen modificado de papa, eIF4E, que contiene mutaciones homólogas a aquellas de la variante alélica resistente recesiva encontrada en pimiento. La expresión de esta variante eIF4E confiere respuestas de resistencia diferencial contra tres variantes prevalentes de PVY incluyendo PVYO, PVYN:O y PVYNTN en las cinco líneas transgénicas probadas. Cuando se estableció la resistencia, el virus no fue detectable en las hojas inoculadas, en las nuevas hojas emergidas, ni en los tubérculos brotados. Algunas variantes se restringieron a un nivel bajo, pero detectable, en algunas de las líneas de papa. Las cruzas entre líneas transformadas y las de tipo silvestre demostraron que el gen de la resistencia asistida era heredable de manera dominante.

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Acknowledgments

We thank Dr. Dennis Halterman for his generous support and Dr. Amy Charkowski for inoculum and critical comments on the manuscript.

This research was supported by the Wisconsin Potato Industry Board (MSN170065 and MSN180863 to AR) and a Hatch Act Formula Fund to J.J.

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Correspondence to Aurélie M. Rakotondrafara.

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Arcibal, E., Morey Gold, K., Flaherty, S. et al. A Mutant eIF4E Confers Resistance to Potato Virus Y Strains and is Inherited in a Dominant Manner in the Potato Varieties Atlantic and Russet Norkotah. Am. J. Potato Res. 93, 64–71 (2016). https://doi.org/10.1007/s12230-015-9489-x

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  • DOI: https://doi.org/10.1007/s12230-015-9489-x

Keywords

  • Potyviridae
  • pvr1 2
  • Recessive resistance
  • Dominant inheritance
  • eIF4E
  • Potato virus Y