American Journal of Potato Research

, Volume 90, Issue 5, pp 440–450 | Cite as

Analysis of Polyphenols, Anthocyanins and Carotenoids in Tubers from Solanum tuberosum Group Phureja, Stenotomum and Andigena

  • Syamkumar S. Pillai
  • Duroy A. Navarre
  • John Bamberg


Sixty-five Solanum tuberosum group Andigena, Phureja and Stenotomum genotypes from an initial population of 1,500 were analyzed for phenylpropanoids, carotenoids, and antioxidant capacity. Total phenolic content ranged from 3 to 49 mg g−1 DW, total carotenoids from 4.1 to 154 μg/g DW, anthocyanins from 0.27 to 34 mg g−1 DW and antioxidant capacity from 60 to 1,767 μmol TE/g DW. HPLC analysis of phenolic extracts revealed that 5-O-chlorogenic acid (5CGA) was the most abundant polyphenol in all genotypes. Ten genotypes were independently grown out for more in-depth phytonutrient analysis. The Phureja genotypes RN 27.01 had the highest polyphenol, anthocyanin and antioxidant content, while RN 39.05 had the highest carotenoid content. The tuber percentage dry matter varied markedly among the ten genotypes, influencing the phytonutrient values when expressed on a dry weight basis. Chlorogenic acid concentrations ranged from 1.7 to 29.4 mg g−1 DW and kaempferol-3-rutinose was present up to 3 mg g−1 DW. Petunidin-3-O-coum-rutinoside-5-O-glu or pelargonidin-3-O-coum-rutinoside-5-O-glu were the most abundant anthocyanins. The principal carotenoids were lutein, zeaxanthin, violaxanthin, and antheraxanthin, but no one carotenoid was predominant in all genotypes. These findings further support utilization of Phureja group germplasm for phytonutrient enhancement efforts.


Anthocyanin Antioxidants Carotenoids Chlorogenic acid Flavonols Phenolics Phytonutrients Potato Phureja Solanum tuberosum 


Se analizaron 65 genotipos de Solanum tuberosum grupo Andigena, Phureja y Stenotomum, de una población inicial de 1500, para fenilpropanoides, carotenoides y capacidad antioxidante. El contenido total de fenoles varió de 3 a 49 mg g-1 de peso seco (PS), los carotenoides totales de 4.1 a 154 μg/g de PS, antocianinas de 0.27 a 34 mg g-1 PS y la capacidad antioxidante de 60-1,767 μmol TE/g PS. El análisis de los extractos fenólicos por cromatografía de líquidos de alto comportamiento (HPLC) reveló que ácido 5-O-clorogénico (5CGA) era el polifenol más abundante en todos los genotipos. Se cultivaron independientes diez genotipos para un análisis más a profundidad de fitonutrientes. Los genotipos de Phureja RN 27.01 tuvieron el mayor contenido de polifenol, antocianina y antioxidantes, mientras que RN 39.05 tuvo el mayor contenido de carotenoides. El porcentaje de materia seca de tubérculo varió marcadamente entre los diez genotipos, influenciando los valores de los fitonutrientes cuando se expresaron con base a peso seco. Las concentraciones de ácido clorogénico variaron de 1.7-29.4 mg g-1 PS y kaempferol-3-rutinosa estuvo presente hasta en 3 mg g-1 PS. Las antocianinas más abundantes fueron Petunidina-3-O-coum-rutinosida-5-O-glu o pelargonidina-3-O-coum-rutinosida-5-O-glu. Los principales carotenoides fueron luteína, zeaxantina, violaxantina y anteraxantina, pero ninguno de los carotenoides fue predominante en todos los genotipos. Estos resultados respaldan aún más la utilización del germoplasma del grupo Phureja para los esfuerzos del incremento en fitonutrientes.

Supplementary material

12230_2013_9318_MOESM1_ESM.doc (36 kb)
Supplemental Table 1 (DOC 36 kb)


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Copyright information

© Potato Association of America 2013

Authors and Affiliations

  • Syamkumar S. Pillai
    • 1
  • Duroy A. Navarre
    • 2
  • John Bamberg
    • 3
  1. 1.Irrigated Agriculture and Extension Research CenterWashington State UniversityProsserUSA
  2. 2.USDA-Agricultural Research ServiceWashington State UniversityProsserUSA
  3. 3.Vegetable Crops Research UnitUSDA-Agricultural Research ServiceSturgeon BayUSA

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