American Journal of Potato Research

, Volume 90, Issue 4, pp 369–377

Evaluating Incidence of Helminthosporium solani and Colletotrichum coccodes on Asymptomatic Organic Potatoes and Screening Potato Lines for Resistance to Silver Scurf

  • Chakradhar Mattupalli
  • Ruth K. Genger
  • Amy O. Charkowski
Article

Abstract

Silver scurf, caused by Helminthosporium solani, and black dot, caused by Colletotrichum coccodes, cause tuber blemishes on potato (Solanum tuberosum L.) which affect processing and fresh market trade. Tubers from ten cultivars were collected at harvest from three organic farms in Wisconsin and categorized as symptomatic or asymptomatic based on visual symptoms of silver scurf and black dot and/or signs of H. solani and C. coccodes. Tuber incubation and PCR assays were performed on asymptomatic tubers to detect H. solani and C. coccodes. Tuber incubation and PCR assays were in slight to fair agreement (kappa coefficient <0.4) for detecting both pathogens. Most asymptomatic tubers tested were positive by one or both assays for H. solani (75 %) or C. coccodes (94 %). Minituber inoculation assays were also performed to screen potato lines for resistance to silver scurf. Of the 14 lines tested, a diploid interspecific hybrid, C287, had consistently low sporulation, suggesting it has partial resistance to silver scurf. Since the majority of tubers harvested are already infected with one or both pathogens further research should focus on organically acceptable management practices that may inhibit disease development in field and in storage.

Keywords

Black dot Minitubers C287 

Resumen

La mancha plateada, causada por Helminthosporium solani y la mancha negra, por Colletotrichum coccodes, originan manchas en los tubérculos de papa (Solanum tuberosum L.), que afectan el intercambio de mercado de procesamiento y en fresco. Se colectaron tubérculos de diez variedades a la cosecha de tres unidades de producción orgánica en Wisconsin, y se clasificaron como sintomáticas y asintomáticas con base en síntomas visuales de mancha plateada o negra y/o con señales de H. solani y C. coccodes. Se condujeron incubaciones de tubérculo y ensayos de PCR en tubérculos asintomáticos para la detección de H. solani y C. coccodes. La incubación de los tubérculos y los ensayos de PCR coincidieron de ligera a consistentemente (coeficiente de Kappa <0.4) en la detección de ambos patógenos. La mayoría de los tubérculos asintomáticos probados fueron positivos por uno o ambos ensayos para H. solani (75 %) o C. coccodes (94 %). También se efectuaron ensayos de inoculación en minitubérculos para seleccionar líneas de papa para la resistencia de la mancha plateada. De las 14 líneas probadas, un híbrido interespecífico diploide, C287, tuvo baja esporulación consistentemente, sugiriendo que tiene resistencia parcial a la mancha plateada. Considerando que la mayoría de los tubérculos cosechados ya están infectados con uno o ambos patógenos, la investigación futura se podría enfocar en prácticas de manejo orgánicamente aceptables que pudieran inhibir el desarrollo de la enfermedad en el campo y en el almacén.

Supplementary material

12230_2013_9314_Fig3_ESM.jpg (47 kb)
Supplementary Fig. 1

Conidia and Conidiophores of Helminthosporium solani (left side) and sclerotia of Colletotrichum coccodes (right side) observed in close proximity on a potato tuber periderm under a dissecting microscope (JPEG 47 kb)

12230_2013_9314_MOESM1_ESM.tif (1.7 mb)
ESM 1(TIFF 1773 kb)

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Copyright information

© Potato Association of America 2013

Authors and Affiliations

  • Chakradhar Mattupalli
    • 1
  • Ruth K. Genger
    • 1
  • Amy O. Charkowski
    • 1
  1. 1.Department of Plant PathologyUniversity of Wisconsin-MadisonMadisonUSA

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