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Recent Advances on Potato virus Y Research in Canada

  • POTATO VIRUS Y SYMPOSIUM
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Abstract

Significant progress has been made in recent years in understanding pathological, serological and molecular properties of various strains of PVY and the aphid-mediated transmission. PVYO and PVYN appear to be the basic strain groups. Through genome recombination between these two groups, progeny groups whose genome possess one (e.g., PVYN:O or PVYN-Wi) to three (e.g., recombinant PVYNTN or European-PVYNTN) recombinant joints of PVYO and PVYN emerged. PVYN:O causes PVYN-like veinal necrosis in tobacco, but reacts to PVYO-specific antibody. PVYNTN causes potato tuber necrotic ringspot diseases in sensitive potato cultivars, and PVYN-like necrosis in tobacco plants, and reacts to PVYN-specific antibody. Through single nucleotide mutation(s), non-recombinant PVYNTN (or North American PVYNTN) also emerged from PVYN. It is also noteworthy that PVYN isolates originated from North America and Europe may have evolved separately; and to date most recombinant strains appear to be progenies of Eu-PVYN and PVYO. Several RT-PCR-based methodologies have been developed to characterize and detect various strains of PVY. A field survey revealed that PVYN:O has become a predominant strain in Manitoba and neighbouring states in USA. Moreover, three distinct variant groups inciting severe, intermediate and mild veinal/petiole/stem necrosis, respectively, on tobacco plants were observed within the PVYN:O isolates collected in Manitoba. Pathological and molecular diversity within PVYO strain group were revealed in New Brunswick, represented by PVYO-FL as a severe variant and by PVYO-RB as a mild variant. Studies on the transmission of PVY by various species of aphids revealed that aphid behavior plays an important role in the vector-mediated transmission. Application of mineral oil on the growing crop, especially in combination with use of crop borders, reduces aphid-mediated PVY transmission. Based on recent PVY research studies, Bartlett Superior 70 Oil was approved in 2011 for application to potato crops in Canada.

Resumen

Se ha hecho progreso significativo en años recientes en el entendimiento de las propiedades patológicas, serológicas y moleculares de variantes del PVY y de su transmisión por áfidos. Parece que PVYO y PVYN son los grupos básicos de las variantes. Mediante la combinación del genomio entre estos dos grupos, han emergido otros grupos de progenie cuyos genomios poseen una (e.g., PVYN:O o PVYN-Wi), a tres (e.g., recombinantes PVYNTN o Europeos-PVYNTN) uniones recombinantes de PVYO y PVYN . El PVYN:O causa necrosis venal en Tabaco como PVYN, pero reacciona al anticuerpo específico de PVYO. El PVYNTN causa enfermedad de las manchas anulares necróticas en el tubérculo de variedades susceptibles de papa, y necrosis como PVYN en plantas de tabaco, y reacciona al anticuerpo específico de PVYN. Por vía de mutación(es) de un solo nucleótido, el PVYNTN no-recombinante (o PVYNTN de Norte América) también emergió del PVYN. También es digno de mención que los aislamientos de PVYN originarios de Norte América y Europa pudieron haber evolucionado por separado; y a la fecha parece que la mayoría de las variantes recombinantes son progenies de Eu-PVYN y PVYO. Se han desarrollado varias metodologías basadas en RT-PCR para caracterizar y detectar varias variantes de PVY. Una auscultación en el campo reveló que PVYN:O se ha vuelto una variante predominante en Manitoba y Estados vecinos en EUA. Más aún, se observaron tres grupos distintos de variantes que incitan necrosis de las venas/pecíolo/tallo severas, intermedias y ligeras, respectivamente, en plantas de tabaco dentro de los aislamientos del PVYN:O colectados en Manitoba. Se reveló diversidad patológica y molecular dentro del grupo de variantes del PVYO en New Brunswick, representada por PVYO-FL como una variante severa y PVYO-RB como ligera. Estudios en la transmisión de PVY por varias especies de áfidos revelaron que el comportamiento del áfido juega un papel importante en la transmisión mediada por el vector. La aplicación de aceite mineral en el cultivo en crecimiento, especialmente en combinación con el uso de cultivos de bordo, reduce la transmisión del PVY mediada por el áfido. Con base en estudios recientes, se aprobó el aceite Bartlett Superior 70 en 2011 para su aplicación en cultivos de papa en Canadá.

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Acknowledgments

The authors are grateful to former and current members in their respective laboratories: T. Molen, A. Dilworth, C. He, Y. Bai, X. Hu, B. Nie, A. Josselyn, N. Manson, M.-A. Giguère, U. Nanayakkara, J. Zhang, T. Henderson, D. Hausermann and T. Kerley. The authors also thank Dr. Gilles Boiteau for critically reviewing the manuscript and for helpful discussion.

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Authors and Affiliations

  1. Potato Research Centre, Agriculture and Agri-Food Canada, P.O. Box 20280, 850 Lincoln Road, Fredericton, New Brunswick, E3B 4Z7, Canada

    Xianzhou Nie & Yvan Pelletier

  2. Agricultural Certification Services, 1030 Lincoln Road, Fredericton, New Brunswick, E3B 8B7, Canada

    Mathuresh Singh

  3. Brandon Research Centre, Agriculture and Agri-Food Canada, 2701 Grand Valley Road, R.R. #3, PO Box 1000A, Brandon, Manitoba, R7A 5Y3, Canada

    Debra McLaren

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  1. Xianzhou Nie
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Correspondence to Xianzhou Nie.

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This paper is an invited presentation as part of the International Symposium on PVY sponsored by the Plant Protection Section of the Potato Association of America, August 11, 2009, in Fredericton, New Brunswick, Canada. This paper was reviewed with a view to ensure that the information was brought up-to-date beyond what was given at the time of the presentation.

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Nie, X., Singh, M., Pelletier, Y. et al. Recent Advances on Potato virus Y Research in Canada. Am. J. Potato Res. 90, 14–20 (2013). https://doi.org/10.1007/s12230-012-9288-6

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