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Response of Colletotrichum Coccodes to Selected Fungicides Using a Plant Inoculation Assay and Efficacy of Azoxystrobin Applied by Chemigation

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Abstract

A plant inoculation assay was developed and used to evaluate fungicide efficacy and application timing for reduction of infection of potato stems by Colletotrichum coccodes. Incidence of infected stems treated with azoxystrobin, pyraclostrobin, fluoxastrobin, and mandipropamid + difenoconazole was significantly less than the non-treated control plants when fungicides were applied prior to inoculation. However, fungicide application after inoculation did not significantly reduce infection. Chlorothalonil and mancozeb were not effective in preventing infection by C. coccodes. Additionally, early season fungicide applications by center pivot chemigation in commercial potato fields were evaluated as a potential black dot management tactic. Below-ground stems sampled from replicated plots in commercial fields where azoxystrobin was applied by chemigation at 50 and 67 days after planting (DAP) had significantly less stem surface area covered with sclerotia at 79 DAP than the non-treated control in two of 2 years. However, the effect was not observed both years at a subsequent collection at 102 DAP and only one of 2 years at 140 DAP. Latent infections were detected in non-symptomatic plants collected from the field.

Resumen

Se desarrolló y utilizó un ensayo de inoculación de plantas para evaluar la eficacia y tiempo de aplicación de fungicida para la reducción de la infección en tallos de papa por Colletotrichum coccodes. La incidencia de tallos infectados tratados con azoxystrobin, pyraclostrobin, fluoxastrobin y mandipropamid + difenoconazole, fue significativamente menor que las plantas testigo no tratadas cuando se aplicaron los fungicidas antes de la inoculación. No obstante, la aplicación del fungicida después de la inoculación no redujo significativamente la infección. Clorotalonil y mancozeb no fueron efectivos en prevenir la infección por C. coccodes. Adicionalmente, se evaluaron aplicaciones de fungicidas al principio del ciclo de cultivo por quimirrigación de pivote central en campos comerciales de papa, como táctica potencial de manejo de la mancha negra. Los tallos subterráneos muestreados de lotes con repeticiones en campos comerciales donde se aplicó azoxistrobin por quimirrigación a 50 y 67 días después de la siembra (DAP), tuvieron significativamente menos área superficial del tallo cubierta con esclerocios a 79 DAP, que los testigos no tratados en dos años. No obstante, no se observó el efecto en ambos años en una colecta posterior a 102 DAP, y solo en uno de los dos años a 140 DAP. Se detectaron infecciones latentes en plantas asintomáticas colectadas en el campo.

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References

  • Andrivon, D., J.M. Lucas, C. Guerin, and B. Jouan. 1998. Colonization of roots, stolons, tubers and stems of various potato (Solanum tuberosum) cultivars by the black dot fungus Colletotrichum coccodes. Plant Pathology 47: 440–445.

    Article  Google Scholar 

  • Byrne, J.M. 1997. Efficacy and economics of management strategies to control anthracnose fruit rot in processing tomatoes in the midwest. Plant Disease 81: 1167–1172.

    Article  Google Scholar 

  • Cummings, T.F., and D.A. Johnson. 2008. Effectiveness of early-season, single applications of azoxystrobin for the control of potato black dot as evaluated by three assessment methods. American Journal of Potato Research 85: 422–431.

    Article  CAS  Google Scholar 

  • Denner, F.D.N., C. Millard, A. Geldenhuys, and F.C. Wehner. 1997. Treatment of seed potatoes with Prochloraz for simultaneous control of silver scurf and black dot on progeny tubers. Potato Research 40: 221–227.

    Article  CAS  Google Scholar 

  • Dillard, H.R. 1988. Influence of temperature, pH, osmotic potential, fungicide sensitivity on germination of conidia and growth from sclerotia of Colletotrichum coccodes in vitro. Phytopathology 78: 1357–1361.

    Article  CAS  Google Scholar 

  • Dillard, H. 1992. Colletotrichum coccodes: the pathogen and its hosts. In Colletotrichum: Biology, pathology and control, 1st ed, ed. J.A. Bailey and M.J. Jeger, 225–236. Wallingford: CAB International.

    Google Scholar 

  • Dillard, H.R., and A.C. Cobb. 1997. Disease progress of black dot on tomato roots and reduction in incidence with foliar applied fungicides. Plant Disease 81: 1439–1442.

    Article  Google Scholar 

  • Dunnett, C.W. 1955. A multiple comparisons procedure for comparing several treatments with a control. Journal of American Statistical Association 509: 1096–1121.

    Article  Google Scholar 

  • Fulton, J.P. 1948. Infection of tomato fruits by Colletotrichum phomoides. Phytopathology 38: 235–246.

    Google Scholar 

  • Geary, B., D.A. Johnson, P.B. Hamm, and T.F. Cummings. 1999. Fungicide application for late blight management: a boom attached to a center pivot irrigation system. Plant Disease 83: 512–515.

    Article  CAS  Google Scholar 

  • Glais, I., and D. Andrivon. 2004. Deep sunken lesion—an atypical symptom on potato tubers caused by Colletotrichum coccodes during storage. Plant Pathology 53: 254.

    Article  Google Scholar 

  • Hamm, P.B., T.F. Cummings, and D.A. Johnson. 2006. Comparison of deposition patterns in two programs for applying protectant fungicides to potato stems and leaves for the control of late blight (Phytophthora infestans). American Journal of Potato Research 83: 473–484.

    Article  CAS  Google Scholar 

  • Heilmann, L.J., N. Nitzan, D.A. Johnson, J.S. Pasche, C. Doetkott, and N.C. Gudmestad. 2006. Genetic variability in the potato pathogen Colletotrichum coccodes as determined by amplified fragment length polymorphism and vegetative compatibility group analyses. Phytopathology 96: 1097–1107.

    Article  PubMed  CAS  Google Scholar 

  • Hunger, R.M., and G.A. McIntyre. 1979. Occurrence, development, and losses associated with silver scurf and black dot on Colorado potatoes. American Potato Journal 56: 289–306.

    Article  Google Scholar 

  • Illman, W.I., R.A. Ludwig, and J. Farmer. 1959. Anthracnose of canning tomatoes in Ontario. Canadian Journal of Botany 37: 1237–1246.

    Article  Google Scholar 

  • Ingram, J., and D.A. Johnson. 2010. Colonization of potato roots and stolons by Colletotrichum coccodes from tuberborne inoculum. American Journal of Potato Research 87: 382–387.

    Article  Google Scholar 

  • Johnson, D.A. 1994. Effect of foliar infection caused by Colletotrichum coccodes on yield of Russet Burbank potato. Plant Disease 78: 1075–1078.

    Article  Google Scholar 

  • Johnson, D.A., and E.R. Miliczky. 1993. Distribution and development of black dot, Verticillium wilt, and powdery scab on Russet Burbank potatoes in Washington State. Plant Disease 77: 74–79.

    Article  Google Scholar 

  • Johnson, D.A., R.C. Rowe, and T.F. Cummings. 1997. Incidence of Colletotrichum coccodes in certified potato seed tubers planted in Washington State. Plant Disease 81: 1199–1202.

    Article  Google Scholar 

  • Johnson, D.A., T.F. Cummings, and P.B. Hamm. 2000. Cost of fungicides used to manage potato late blight in the Columbia Basin: 1996 to 1998. Plant Disease 84: 399–402.

    Article  Google Scholar 

  • Kendrick Jr., J.B., and J.C. Walker. 1948. Anthracnose of tomato. Phytopathology 38: 247–260.

    Google Scholar 

  • Kotcon, J.B., D.I. Rouse, and J.E. Mitchell. 1985. Interactions of Verticillium dahliae, Colletotrichum coccodes, Rhizoctonia solani, and Pratylenchus penetrans in the early dying syndrome of Russet Burbank potatoes. Phytopathology 75: 68–74.

    Article  Google Scholar 

  • Miller, J.S., and B.G. Hopkins. 2008. Checklist for a holistic potato health management plan. In Potato health management, 2nd ed, ed. D.A. Johnson, 7–10. St Paul: APS.

    Google Scholar 

  • Mohan, S.K., J.R. Davis, L.H. Sorensen, and A.T. Schneider. 1992. Infection of aerial parts of potato plants by Colletotrichum coccodes and its effects on premature vine death and yield. American Potato Journal 69: 547–559.

    Article  Google Scholar 

  • Nitzan, N., T.F. Cummings, and D.A. Johnson. 2005. Effect of seed-tuber generation, soilborne inoculum, and azoxystrobin application on development of potato black dot caused by Colletotrichum coccodes. Plant Disease 89: 1181–1185.

    Article  CAS  Google Scholar 

  • Nitzan, N., M. Evans, and D.A. Johnson. 2006a. Colonization of potato plants after aerial infection by Colletotrichum coccodes, causal agent of potato black dot. Plant Disease 90: 999–1003.

    Article  Google Scholar 

  • Nitzan, N., B.S. Lucas, and B.J. Christ. 2006b. Colonization of rotation crops and weeds by the potato black dot pathogen Colletotrichum coccodes. American Journal of Potato Research 83: 503–507.

    Article  Google Scholar 

  • Nitzan, N., L. Tsror, and D.A. Johnson. 2006c. Vegetative compatibility groups and aggressiveness of North American isolates of Colletotrichum coccodes, the causal agent of potato black dot. Plant Disease 90: 1287–1292.

    Article  Google Scholar 

  • Nitzan, N., T.F. Cummings, and D.A. Johnson. 2008. Disease potential of soil- and tuberborne inocula of Colletotrichum coccodes and black dot severity on potato. Plant Disease 92: 1497–1502.

    Article  Google Scholar 

  • Nitzan, N., M.A. Evans, T.F. Cummings, D.A. Johnson, D.L. Batchelor, C. Olsen, K.G. Haynes, and C.R. Brown. 2009. Field resistance to potato stem colonization by the black dot pathogen Colletotrichum coccodes. Plant Disease 93: 1116–1122.

    Article  Google Scholar 

  • Otazu, V., N.C. Gudmestad, and R.T. Zink. 1978. The role of Colletotrichum atramentarium in the potato wilt complex in North Dakota. Plant Disease Reporter 62: 847–851.

    Google Scholar 

  • Pasche, J.S., R.J. Taylor, and N.C. Gudmestad. 2010. Colonization of potato by Colletotrichum coccodes: Effect of soil infestation and seed tuber and foliar inoculation. Plant Disease 94: 905–914.

    Article  Google Scholar 

  • Porter, L.D., J.S. Miller, P. Nolte, and W.J. Price. 2006. In vitro somatic growth and reproduction of phenylamide-resistant and -sensitive isolates of Phytophthora erythroseptica from infected potato tubers in Idaho. Plant Pathology: 1–8.

  • Raid, R.N., and S.P. Pennypacker. 1987. Weeds as hosts of Colletotrichum coccodes. Plant Disease 71: 643–646.

    Article  Google Scholar 

  • Read, P.J., and G.A. Hide. 1995. Development of black dot disease (Colletotrichum coccodes (Wallr.) Hughes) and its effects on the growth and yield of potato plants. The Annals of Applied Biology 127: 57–72.

    Article  Google Scholar 

  • Scholte, K., J.W. Veenbaas-Rijks, and R.E. Labruyere. 1985. Potato growing in short rotations and the effect of Streptomyces spp., Colletotrichum coccodes, Fusarium tabacinum and Verticillium dahliae on plant growth and tuber yield. Potato Research 28: 331–348.

    Article  Google Scholar 

  • Stevenson, W.R., R.J. Green, and G.B. Bergeson. 1976. Occurrence and control of potato black dot root rot in Indiana. Plant Disease Reporter 60: 248–251.

    Google Scholar 

  • Stevenson, W.R., W.W. Kirk, and Z.K. Atallah. 2008. Managing foliar diseases: Early blight, late blight and white mold. In Potato health management, 2nd ed, ed. D.A. Johnson, 209–222. St. Paul: APS.

    Google Scholar 

  • Tsror (Lahkim), L., and M. Hazanovsky. 2001. Effect of coinfection by Verticillium dahliae and Colletotrichum coccodes on disease symptoms and fungal colonization in four potato cultivars. Plant Pathology 50: 483–488.

    Article  Google Scholar 

  • Tsror, L., O. Erlich, and M. Hazanovsky. 1999. Effect of Colletotrichum coccodes on potato yield, tuber quality, and stem colonization during spring and autumn. Plant Disease 83: 561–565.

    Article  Google Scholar 

  • Uribe, E., and R. Loria. 1994. Response of Colletotrichum coccodes to fungicides in vitro. American Potato Journal 71: 455–465.

    Article  Google Scholar 

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Acknowledgments

We thank Gary Grove, Mark Pavek and Nadav Nitzan for critical reviews of the manuscript, and Donald Drader of Syngenta and Heath Gimmestad of Friehe Farms for providing support with the field work. PPNS no. 0555, Department of Plant Pathology, College of Agricultural, Human, and Natural Resource Sciences Agricultural Research Center, Project No. WNPO 0678, Washington State University, Pullman, WA 99164–6430, USA.

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  1. Department of Plant Pathology, Washington State University, Pullman, WA, 99164, USA

    Jason Ingram, Thomas F. Cummings & Dennis A. Johnson

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  1. Jason Ingram
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  2. Thomas F. Cummings
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Correspondence to Dennis A. Johnson.

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Ingram, J., Cummings, T.F. & Johnson, D.A. Response of Colletotrichum Coccodes to Selected Fungicides Using a Plant Inoculation Assay and Efficacy of Azoxystrobin Applied by Chemigation. Am. J. Pot Res 88, 309–317 (2011). https://doi.org/10.1007/s12230-011-9195-2

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