Abstract
Dental caries is a biofilm-related disease, widely perceived to be caused by oral ecological imbalance when cariogenic/aciduric bacteria obtain an ecological advantage. Compared with planktonic bacteria, dental plaques are difficult to remove under extracellular polymeric substance protection. In this study, the effect of caffeic acid phenethyl ester (CAPE) on a preformed cariogenic multi-species biofilm was evaluated, which was comprised of cariogenic bacteria (Streptococcus mutans), commensal bacteria (Streptococcus gordonii), and a pioneer colonizer (Actinomyces naeslundii). Our result revealed that treatment with 0.08 mg/mL CAPE reduced live S. mutans in the preformed multi-species biofilm while not significantly changing the quantification of live S. gordonii. CAPE significantly reduced the production of lactic acid, extracellular polysaccharide, and extracellular DNA and made the biofilm looser. Moreover, CAPE could promote the H2O2 production of S. gordonii and inhibit the expression of SMU.150 encoding mutacin to modulate the interaction among species in biofilms. Overall, our results suggested that CAPE could inhibit the cariogenic properties and change the microbial composition of the multi-species biofilms, indicating its application potential in dental caries prevention and management.
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This research was funded by the National Natural Science Foundation of China, grant number 81970931.
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Conceptualization: Xiaohui Lv, Kun Wang, Linglin Zhang; methodology: Xiaohui Lv, Zening Feng, Zhenqi Liu, Junzhuo Lu, Sili Han; formal analysis and investigation: Xiaohui Lv, Zening Feng, Junyuan Luo, Zhenqi Liu; writing—original draft preparation: Xiaohui Lv, Zening Feng, Junyuan Luo; writing—review and editing: Zhenqi Liu, Junzhuo Lu, Sili Han, Kun Wang, Linglin Zhang; funding acquisition: Linglin Zhang; resources: Linglin Zhang; supervision: Linglin Zhang, Kun Wang, Junzhuo Lu, Sili Han. All authors reviewed the manuscript.
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Lv, X., Feng, Z., Luo, J. et al. Effects of caffeic acid phenethyl ester against multi-species cariogenic biofilms. Folia Microbiol 68, 977–989 (2023). https://doi.org/10.1007/s12223-023-01064-w
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DOI: https://doi.org/10.1007/s12223-023-01064-w