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Deletion of the cheZ gene results in the loss of swimming ability and the decrease of adhesion ability to Caco-2 cells in Escherichia coli Nissle 1917

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Abstract

The aim of this study was to elucidate the biological functions of the motility regulatory protein CheZ in the probiotic strain Escherichia coli Nissle 1917. A cheZ gene deletion strain Nissle 1917ΔcheZ was constructed using the CRISPR/Cas9 two-plasmid system, and the corresponding complemented strain Nissle 1917ΔcheZ/pBR322-cheZ was established. Combined studies of growth kinetics testing, motility assays, swarming motility assays, and bacterial adherence assays were performed to study the motility regulatory protein CheZ-mediated functions in the prototype Nissle 1917 strain, its isogenic cheZ mutant, and the corresponding complemented strain. The growth rate of the cheZ mutant strain was lower than that of the wild-type strain in the exponential growth phase. The motility of the cheZ mutant strain was significantly lower than that of the wild-type strain. And the adhesion ability of ΔcheZ mutant to the Caco-2 cells was significantly lower than that of the wild-type strain and complemented strain. In conclusion, the results presented in our study suggested that the deletion of the cheZ gene in E. coli Nissle 1917 led to a significant reduction of its swimming ability and a subsequent marked decrease of adhesion to the Caco-2 cells.

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Funding

The financial support for this study was provided by the National Natural Science Foundation of China (Grant No. 32102703), Basic and Applied Basic Research Joint Fund of Guangdong Province(2019A1515111186), National College Students’ Innovation and Entrepreneurship Training Plan Program (202110580008), College Students’ Innovation and Entrepreneurship Training Plan Program of Guangdong Province (S202010580051, S202210580052), Scientific Research Start-up Fund of Zhaoqing University (611/180160), the Provincial Regular University Key Research Platform and Project Foundation of Guangdong (2022KTSCX147), the Third Batch of Innovative Research Team Project of Zhaoqing University (5), Fund of construction of Ecological Poultry Industry Technology System in Guizhou Province (Functional laboratory of Disease Prevention and Control), and the project of introducing talents in Guizhou University (GDRJHZ[2016]77).

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Authors and Affiliations

  1. School of Life Sciences, Zhaoqing University, Zhaoqing, 526061, China

    Bingming Ou, Haihui Lv, Hua Ge, Dexiang Fu, Xin Lin, Saiqiao Huang, Xiaojie Chen, Yufu Liu, Sainan Li, Wenhua Liu, Lihua Huang & Minyu Zhang

  2. College of Animal Science, Guizhou University, Guiyang, 550025, China

    Ying Yang

  3. School of Physical Education & Sports Science, South China Normal University, Guangzhou, 510006, China

    Minyu Zhang

  4. Laboratory of Microbial Resources Exploitation and Application, Zhaoqing University, Zhaoqing, 526061, China

    Bingming Ou & Sainan Li

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  1. Bingming Ou
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  2. Haihui Lv
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  3. Hua Ge
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  12. Ying Yang
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  13. Minyu Zhang
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Correspondence to Ying Yang or Minyu Zhang.

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Ou, B., Lv, H., Ge, H. et al. Deletion of the cheZ gene results in the loss of swimming ability and the decrease of adhesion ability to Caco-2 cells in Escherichia coli Nissle 1917. Folia Microbiol 68, 395–402 (2023). https://doi.org/10.1007/s12223-022-01019-7

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