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Genome sequence and comparative analysis of fungal antagonistic strain Bacillus velezensis LJBV19

Abstract

Bacillus species as fungal antagonistic agents have been widely used in the agriculture and considered as safe products for the management of plant pathogens. In this study, we reported the whole genome sequence of strain LJBV19 isolated from grapevine rhizosphere soil. Strain LJBV19 was identified as Bacillus velezensis through morphological, physicochemical, molecular analysis and genome comparison. Bacillus velezensis LJBV19 had a significant inhibitory effect on the growth of Magnaporthe oryzae with an inhibition ratio up to 75.55% and showed broad spectrum of activity against fungal phytopathogens. The 3,973,013-bp circular chromosome with an average GC content of 46.5% consisted of 3993 open reading frames (ORFs), and 3308 ORFs were classified into 19 cluster of orthologous groups of proteins (COG) categories. Genes related to cell wall degrading enzymes were predicted by Carbohydrate-Active enZYmes (CAZy) database and validated at the metabolic level, producing 0.53 ± 0.00 U/mL cellulose, 0.14 ± 0.01 U/mL chitinase, and 0.11 ± 0.01 U/mL chitosanase. Genome comparison confirmed the taxonomic position of LJBV19, conserved genomic structure, and genetic homogeneity. Moreover, 13 gene clusters for biosynthesis of secondary metabolites in LJBV19 genome were identified and two unique clusters (clusters 2 and 12) shown to direct an unknown compound were only present in strain LJBV19. In general, our results will provide insights into the antifungal mechanisms of Bacillus velezensis LJBV19 and further application of the strain.

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Availability of data and material

The GenBank accession number for the 16S rRNA gene of Bacillus velezensis LJBV19 is MZ157279. The complete sequence of the LJBV19 has been deposited in GenBank database under the accession number CP072563. The strain B. velezensis LJBV19 was publicly available from China General Microbiological Culture Collection Center (CGMCC) under the accession number CGMCC No. 21804. Morphological characteristics, phylogenetic tree, effect of LJBV19 on the mycelia of Botrytis cinerea, the location and products of secondary metabolite gene clusters, physiological and biochemical characteristics, minimum information about the genome sequence, genome-to-genome comparison (ANI and dDDH), and inhibition radius of B. velezensis LJBV19 against 12 pathogens are available as Supplementary Materials.

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Funding

This work was supported by Shanghai Municipal Agricultural Commission (grant no. 2021–02-08–00-12-F00751), National Key R & D Program of China (2019YFD1002501 and 2018YFD1000301), Shanghai Municipal Commission for Science and Technology (18391900400), Yunnan Province Science and Technology Department (202005AF150023 to J. Lu), and China Agriculture Research System (grant no. CARS-29-yc-2).

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All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Bo Wang, Bohan Yang, Hang Peng, and Peining Fu. The first draft of the manuscript was written by Bo Wang and Bohan Yang; all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Peining Fu.

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The authors declare no competing interests.

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Bo Wang and Bohan Yang contributed equally to this work.

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Supplementary file1 (DOCX 2471 KB)

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Wang, B., Yang, B., Peng, H. et al. Genome sequence and comparative analysis of fungal antagonistic strain Bacillus velezensis LJBV19. Folia Microbiol (2022). https://doi.org/10.1007/s12223-022-00996-z

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  • DOI: https://doi.org/10.1007/s12223-022-00996-z

Keywords

  • Bacillus velezensis
  • Fungal antagonistic
  • Genome sequencing
  • Comparative genomics
  • Secondary metabolites