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Purification and characterization of anti-phytopathogenic fungi angucyclinone from soil-derived Streptomyces cellulosae

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Abstract

Actinomycete strain YIM PH20352, isolated from the rhizosphere soil sample of Panax notoginseng collected in WenShang, Yunnan Province, China, exhibited antifungal activity against some phytopathogenic fungi. The structures of bioactive molecules, isolated from the ethyl acetate extract of the fermentation broth of the strain, were identified as rabelomycin (1) and dehydrorabelomycin (2) based on extensive spectroscopic analyses. Compound 1 exhibited antifungal activity against four tested root-rot pathogens of the Panax notoginseng including Plectosphaerella cucumerina, Alternaria panax, Fusarium oxysporum, and Fusarium solani with the MIC values at 32, 64, 128, and 128 μg/mL, respectively. Compound 2 exhibited antifungal activity against F. oxysporum, P. cucumerina, F. solani, and A. panax with the MIC values at 64, 64, 128, and 128 μg/mL, respectively. Based on the phylogenetic analyses, the closest phylogenetic relative of strain YIM PH20352 is Streptomyces cellulosae NBRC 13027 T (AB184265) (99.88%), so strain YIM PH20352 was identified as Streptomyces cellulosae. To the best of our knowledge, this is the first report of rabelomycin and rabelomycin-type antibiotics from Streptomyces cellulosae and their antifungal activity against root-rot pathogens of the Panax notoginseng.

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Funding

This work was supported by the National Natural Science Foundation of China (Grant No. 31660532, 81860634, 81760632).

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Authors and Affiliations

  1. Key Laboratory of Microbial Diversity in Southwest China, Ministry of Education, College of Life Science, Yunnan University, Kunming, 650091, China

    Xindong Xu, Yang Zhao, Kang Bao, Cuiping Miao, Lixing Zhao, Youwei Chen, Shaohua Wu & Yiqing Li

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  1. Xindong Xu
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  2. Yang Zhao
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  3. Kang Bao
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  4. Cuiping Miao
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  5. Lixing Zhao
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  6. Youwei Chen
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  7. Shaohua Wu
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  8. Yiqing Li
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Correspondence to Yiqing Li.

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Xu, X., Zhao, Y., Bao, K. et al. Purification and characterization of anti-phytopathogenic fungi angucyclinone from soil-derived Streptomyces cellulosae. Folia Microbiol 67, 517–522 (2022). https://doi.org/10.1007/s12223-022-00957-6

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  • DOI: https://doi.org/10.1007/s12223-022-00957-6

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