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Evaluation of the antifungal activity of α, β, and δ-damascone and inclusion complexes in β-cyclodextrin against Candida spp

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Abstract

Due to the increase in fungal resistance to existing drugs, a need exists to search for new antifungals. This study aimed to evaluate the antifungal activity of α, β, and δ-damascone and inclusion complexes with β-cyclodextrin against different Candida spp. The inclusion complex of β-damascone was prepared by the co-evaporation method using three molar proportions (1:1; 2:1; 3:1 (βDA-βCD)) and analyzed using Fourier transform infrared spectroscopy (FTIR). Standard Candida albicans (CA INCQS 40,006), Candida krusei (CK INCQS 40,095), and Candida tropicalis (CT INCQS 40,042) strains were used to evaluate antifungal activity. The substances were tested individually or in association with fluconazole (FCZ). The IC50 and cell viability curve constructions were performed using the microdilution method. The minimum fungicidal concentration (MFC) was determined by the subculture method in a solid medium. The α, β, and δ-DA isolated or in combination with fluconazole (FCZ) showed significant antifungal activity. β-damascone showed effective complexation in the three molar proportions assayed; however, none of the inclusion complexes was demonstrated clinically significant effects against the fungal tested. Then, all compounds have shown promising antifungal activities; however, in vivo assays are necessary to have therapeutical application in the future.

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Acknowledgements

Laboratory of Pharmacology and Molecular Chemistry (Laboratório de Farmacologia e Química Molecular; LFQM), Cariri Applied Mycology Laboratory (Laboratório de Micologia Aplicada do Cariri; LMAC), Laboratory of Microbiology and Molecular Biology (Laboratório de Microbiologia e Biologia Molecular; LMBM), and the Laboratory of Simulations and Molecular Spectroscopy (Laboratório de Simulações e Espectroscopia Molecular; LaSEMol) from the Regional University of Cariri (Universidade Regional do Cariri; URCA), for the space to carry out the work.

Funding

This work was supported by Cearense Foundation to Support Scientific and Technological Development (FUNCAP), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brasil (CAPES—Finance Code 001), National Council for Scientific and Technological Development (CNPq) and Financier of Studies and Projects (FINEP). This article is a contribution of the National Institute of Science and Technology - Ethnobiology, Bioprospecting and Nature Conservation/CNPq/FACEPE.

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Authors and Affiliations

  1. Graduate Program in Biotechnology-Northeast Biotechnology Network (RENORBIO), State University of Ceará (UECE), Av. Dr. Silas Munguba, 1700-Itaperi, Fortaleza, Ceará, 60741-000, Brazil

    Maria Rayane Correia de Oliveira

  2. Laboratory of Pharmacology and Molecular Chemistry (LFQM), Department of Biological Chemistry, Regional University of Cariri-URCA, 63.100-000, Pimenta, Crato-Ceará, Brazil

    Maria Rayane Correia de Oliveira, Maria Gabriely de Lima Silva, Francisco Lucas Alves Batista, Roger Henrique Sousa da Costa, Anita Oliveira Brito Pereira Bezerra Martins & Irwin Rose Alencar de Menezes

  3. Laboratory of Applied Mycology of Cariri, Regional University of Cariri-URCA, 63.100-000, Pimenta, Crato-Ceará, Brazil

    Antonia Thassya Lucas dos Santos & Maria Flaviana Bezerra Morais-Braga

  4. Department of Biological Chemistry, Regional University of Cariri, 63.100-000, Pimenta, Crato-Ceará, Brazil

    Beatriz Gonçalves da Cruz, Diniz Maciel de Sena Junior & Alexandre Magno Rodrigues Teixeira

  5. Laboratory of Microbiology and Molecular Biology, Department of Biological Chemistry, Regional University of Cariri-URCA, 63.100-000, Pimenta, Crato-Ceará, Brazil

    Henrique Douglas Melo Coutinho

  6. Laboratory of Bioprospecting of Natural Products and Biotechnology (LBPNB), Center of Education, Sciences and Technology of the Inhamuns Region- CECITEC, State University of Ceará, Tauá, Ceará, CEP, 63660-000, Brazil

    Francisco Ernani Alves Magalhães

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  1. Maria Rayane Correia de Oliveira
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  2. Maria Gabriely de Lima Silva
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Contributions

Conceptualization, I.R.A.d.M., H.D.M.C., M.F.B.M.B., D.S.J F.E.A.M., and A.M.T.; methodology, M.R.C.d.O., M.G.d.L.S., A.T.L.d.S., B.G.d.C., R.H.S.d.C; validation, F.L.A.B; formal analysis, A.T.L.d.S; writing-review and editing, M.R.C.d.O and A.O.B.P.B.M; supervision, I.R.A.d.M, H.D.M.C., and M.F.B.M.B. All authors have read and agreed to the published version of the manuscript.

Corresponding authors

Correspondence to Henrique Douglas Melo Coutinho or Irwin Rose Alencar de Menezes.

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de Oliveira, M.R.C., de Lima Silva, M.G., dos Santos, A.T.L. et al. Evaluation of the antifungal activity of α, β, and δ-damascone and inclusion complexes in β-cyclodextrin against Candida spp. Folia Microbiol 67, 447–457 (2022). https://doi.org/10.1007/s12223-021-00945-2

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