Selective pressure of biphenyl/polychlorinated biphenyls on the formation of aerobic bacterial associations and their biodegradative potential

Egorova, Darya; Kir’yanova, Tatyana; Pyankova, Anna; Anan’ina, Ludmila; Plotnikova, Elena

doi:10.1007/s12223-021-00873-1

Original Article
Published: 08 May 2021

Selective pressure of biphenyl/polychlorinated biphenyls on the formation of aerobic bacterial associations and their biodegradative potential

Darya Egorova ORCID: orcid.org/0000-0001-8018-4687¹,
Tatyana Kir’yanova¹,
Anna Pyankova¹,
Ludmila Anan’ina¹ &
Elena Plotnikova¹

Folia Microbiologica volume 66, pages 659–676 (2021)Cite this article

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Abstract

Unique bacterial associations were formed in the polluted soils from territory of the industrial factories Open Joint Stock Company “The Middle Volga Chemical Plant,” Chapaevsk, Russia and Open Joint Stock Company “Lubricant Producing Plant,” Perm, Russia. This study evaluates the influence of the biphenyl/polychlorinated biphenyls (PCB) on the formation of aerobic bacterial associations and their biodegradative potential. Enrichment cultivation of the soil samples from the territories of these industrial factories with PCB (commercial mixture Sovol) was lead for forming aerobic bacterial enrichment cultures showing a unique composition. The dominating in these bacterial cultures was the phylum Proteobacteria (Beta- and Gammaproteobacteria). Using biphenyl as a carbon source led to decrease of biodiversity in the final stable bacterial associations. Periodic cultivation experiments demonstrated that the association PN2-B has a high degradative potential among the six studied bacterial associations. PN2-B degraded 100% mono-chlorobiphenyls (94.5 mg/L), 86.2% di-chlorobiphenyls (22.3 mg/L), 50.9% Sovol, and 38.4% Delor 103 (13.8 mg/L). Qualitative analysis of metabolites showed that association performed transformation of chlorobenzoic acids (PCB degradation intermediates) into metabolites of citrate cycle. Twelve individual strain-destructors were isolated. The strains were found to degrade 17.7–100% PCB1, 36.2–100% PCB2, 18.8–100% PCB3 (94.5 mg/L), and 15.7–78.2% PCB8 (22.3 mg/L). The strains were shown to metabolize chlorobenzoic acids formed during degradation of chlorobiphenyls. A unique ability of strains Micrococcus sp. PNS1 and Stenotrophomonas sp. PNS6 to degrade ortho-, meta-, and para-monosubstituted chlorobenzoic acids was revealed. Our results suggest that PN2-B and individual bacterial strains will be perspective for cleaning of the environment from polychlorinated biphenyls.

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The sequences for the 16S rRNA gene have been deposited with the GenBank. Accession numbers are presented in the tables in the sect. “Results” and “Discussions.”

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Funding

This research was funded by the grant of Russian Foundation of Basic Research 18-29-05016mk.

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Laboratory of Molecular Microbiology and Biotechnology, Institute of Ecology and Genetics of Microorganism UB RAS, 13 Golev st, Perm, 614081, Russia
Darya Egorova, Tatyana Kir’yanova, Anna Pyankova, Ludmila Anan’ina & Elena Plotnikova

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Darya Egorova
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Tatyana Kir’yanova
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Anna Pyankova
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Ludmila Anan’ina
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Elena Plotnikova
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Darya Egorova—conceptualization, investigation, formal analysis, validation, writing—original draft, funding acquisition; Anna Pyankova, Ludmila Anan’ina—investigation, formal analysis, validation, visualization, writing—original draft; Tatyana Kir’yanova—investigation, formal analysis; Elena Plotnikova—writing—review and editing.

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Egorova, D., Kir’yanova, T., Pyankova, A. et al. Selective pressure of biphenyl/polychlorinated biphenyls on the formation of aerobic bacterial associations and their biodegradative potential. Folia Microbiol 66, 659–676 (2021). https://doi.org/10.1007/s12223-021-00873-1

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Received: 19 January 2021
Accepted: 26 April 2021
Published: 08 May 2021
Issue Date: August 2021
DOI: https://doi.org/10.1007/s12223-021-00873-1