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Ion-selective electrode integrated in small-scale bioreactor for continuous intracellular pH determination in Lactobacillus plantarum

Folia Microbiologica volume 65, pages 467–473 (2020)Cite this article

Abstract

The aim of the present study was to develop an ion-selective electrode method for the continuous determination of the intracellular pH in Lactobacillus plantarum using a small-scale bioreactor. This method employed a salicylate-selective electrode basing on the distribution of salicylic acid across the cytoplasmic membrane. This developed electrode responded to salicylate concentrations above 20 μmol/L with a Nernstian sensitivity. The energized and concentrated cells were added into a thermostated small-scale bioreactor that contained the salicylate anions dissolved in a 100 mmol/L potassium phosphate buffer at different pH values. The changes in salicylate concentration that occurred in the medium containing bacterial suspension were measured as a voltage change. The cells of Lactobacillus plantarum showed maintenance of pH homeostasis at the studied pH ranging from 4.0 to 7.0, and they kept a neutral intracellular pH up to 5.8. The simplicity of the measuring preparation and the relatively low cellular concentration, as well as the advantages of the small-scale bioreactor, lead us to believe that the described method can facilitate the study of the physicochemical factors on the intracellular pH of lactic acid bacteria using a single pH probe in one method.

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Authors and Affiliations

  1. Research Center for Redox Applications in Foods (RCRAF), Department of Food Engineering, Faculty of Engineering, Iğdır University, 76000, Iğdır, Turkey

    Duried Alwazeer

  2. UMR Procédés Alimentaires et Microbiologiques, AgroSup Dijon, 1 Esplanade Erasme, 21000, Dijon, France

    Rémy Cachon

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  1. Duried Alwazeer
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Correspondence to Duried Alwazeer.

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Alwazeer, D., Cachon, R. Ion-selective electrode integrated in small-scale bioreactor for continuous intracellular pH determination in Lactobacillus plantarum. Folia Microbiol 65, 467–473 (2020). https://doi.org/10.1007/s12223-019-00749-5

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  • DOI: https://doi.org/10.1007/s12223-019-00749-5