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Different gut microbiome composition in obese Guizhou minipigs between female and castrated male

Folia Microbiologica volume 64, pages 889–898 (2019)Cite this article

Abstract

Gut microbiome lives in the intestinal tract of animals and plays an important role in almost all life processes. Gut microbiome balance is beneficial to health, and imbalance leads to many diseases, one of which is obesity epidemic. However, gut microbiome is also influenced by host hormone, and different gut microbiome composition is observed between the sexes. Here, we studied whether castrated male Guizhou minipigs with obesity own the same gut microbiome composition and microbial function predictions with those in obese females. We sequenced the hypervariable regions V3 to V4 of bacterial 16s rRNA of fecal samples collected from our study subjects. We observed that the operational taxonomic units were small, which suggested that the abundance of gut microbiome may be influenced by low genetic diversity of host. Our results also suggested that the castrated male has different gut microbial composition compared to the obese female. An increasing Firmicutes/Bacteroidetes ratio was observed in both castrated male and obese female groups, which suggested that the main adipogenic gut microorganism in obese Guizhou minipigs in our studies is the same with that in other obese mammals. However, we also observed that there were function prediction differences of obese Guizhou minipigs between female and castrated male, which suggested that the influence of gut microbiome on obesity between them is different.

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Acknowledgements

We are grateful for everyone who contributed to our project and appreciate Shanghai Majorbio Bio-pharm Technology Co., Ltd. for their sequencing and data analysis on cloud platform. We thank Yanjun Wu and Arome Solomon Odiba for the English revision.

Funding

The study is supported by fund from central special fund for local science and technology development [Qian Ke Zhong Yin Di [2016] 4007], fund from project for growth of youth researcher of Education department of Guizhou province [Qian Jiao He KY No. [2016] 182], and fund from the Administration of Traditional Chinese Medicine of Guizhou Province.

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  1. Gang Yao and Shuguang Wu contributed equally to this work.

Affiliations

  1. Institute of Laboratory Animal Science, Guizhou University of Traditional Chinese Medicine, Huaxi University Town, Guiyang, Guizhou, People’s Republic of China

    Gang Yao, Shuguang Wu, Hai Zhao, Guoqi Wang, Mingfei Chen & Ning Qian

  2. Guizhou Provincial People’s Hospital, Guiyang, Guizhou, People’s Republic of China

    Xianchun Zeng

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  1. Gang Yao
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Correspondence to Shuguang Wu.

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Yao, G., Wu, S., Zeng, X. et al. Different gut microbiome composition in obese Guizhou minipigs between female and castrated male. Folia Microbiol 64, 889–898 (2019). https://doi.org/10.1007/s12223-019-00704-4

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